Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Self-powered microtubule-kinesin transport system and preparation method thereof

A technology of kinesin and self-supply energy, which is applied in chemical instruments and methods, animal/human peptides, material inspection products, etc. It can solve the problems of activity reduction, kinesin and microtubule protein damage, cumbersome operation steps, etc.

Inactive Publication Date: 2014-09-03
INST OF CHEM CHINESE ACAD OF SCI
View PDF8 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Some researchers have successfully realized the supply of ATP in the kinesin-microtubule transport system through ultraviolet light, electric field or microfluidic technology, but ultraviolet light and electric field will cause damage to kinesin and microtubule protein, the activity will be reduced, and energy transfer cannot be realized. Continuous supply; while microfluidic technology requires repeated replacement of ATP buffer to achieve continuous supply of energy, and the operation steps are cumbersome

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Self-powered microtubule-kinesin transport system and preparation method thereof
  • Self-powered microtubule-kinesin transport system and preparation method thereof
  • Self-powered microtubule-kinesin transport system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0035] The preparation method of the silanized fluid cell is the same as the above method for preparing the common fluid cell, except that the slide glass and the cover glass need to be silanized in advance. Preparation of aminosilanized coverslips: 20 μl of 3-aminopropyltriethoxysilane (5 wt %) in acetone was dropped onto a clean slide and immediately covered with another clean slide. After 5 min, the slides were rinsed with water and dried with liquid nitrogen. This results in two aminosilanized coverslips. Prepare a fluid cell with the 2 coverslips described above.

[0036] In the following examples, the preparation method of the rhodamine-labeled biotin-modified microtubules used is: rhodamine-labeled microtubules, biotin-modified microtubules and unmodified microtubules (volume ratio 1:1:2 ) mixed well and dispersed in 4mM MgCl 2 , 1mM GTP (guanosine triphosphate) and 5wt% DMSO in the BRB80 buffer solution, the total protein concentration was 2.5mg / ml; then placed in a...

Embodiment 1

[0039] Example 1, preparation of microtubule-kinesin transport system

[0040] (1) Preparation of creatine kinase (CPK) microspheres

[0041] Take Na 2 CO 3 Water and CPK aqueous solution were placed in a round bottom flask, and then an equal volume of CaCl was quickly added at one time 2 Put the aqueous solution in a flask, stir it for 20 seconds immediately, let it stand for about 2 minutes, take the precipitate, centrifuge it, and wash it with water three times to obtain CPK microspheres.

[0042]The scanning electron micrographs of the CPK microspheres prepared above are as follows: figure 1 As shown in (a), the laser confocal micrograph is as follows figure 1 As shown in (b), it can be seen from this figure that the particle size of the CPK microspheres prepared in this embodiment is 3.6 μm.

[0043] (2) Preparation of Microtubule-Creatine Kinase (MT-CPK) Microsphere Complex

[0044] The creatine kinase microspheres prepared in step (1) were dispersed into polyallyl...

Embodiment 2

[0051] Example 2, preparation of microtubule-kinesin transport system

[0052] The steps and parameters of this embodiment are basically the same as in Example 1, the difference is that when preparing creatine kinase microspheres, the Na 2 CO 3 Aqueous solution or CaCl 2 A drug model (fluorescein isothiocyanate-dextran (FITC-Dextran)) was added to the aqueous solution, and creatine kinase-drug composite microspheres were obtained by co-precipitation. Therefore, the composite microspheres prepared in this example can not only continuously provide ATP for the microtubule-kinesin transport system, but also serve as a carrier to transport drugs or other substances.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention discloses a self-powered microtubule-kinesin transport system and a preparation method thereof. The preparation method comprises the following steps: (1) preparing a kinase sphere from kinase; (2) respectively preparing a kinase sphere-microtubule compound or a kinase sphere-kinesin compound according to a step <1> or <2>; and (3) preparing according to the steps <1> or <2>, thereby obtaining the self-powered microtubule-kinesin transport system, wherein in the step <1>, the kinase sphere-microtubule compound, casein and kinesin are mixed to obtain the self-powered microtubule-kinesin transport system, and in the step <2>, the kinase sphere-kinesin compound and a microtubule are mixed to obtain the self-powered microtubule-kinesin transport system. The microtubule-kinesin transport system disclosed by the invention can be self-powered, and no extra ATP (Adenosine Triphosphate) is needed, so that the system is beneficial to a sealed nano device system.

Description

technical field [0001] The invention relates to a self-powered microtubules-kinesin transport system and a preparation method thereof. Background technique [0002] Life lies in movement, and all activities of the body, from muscle contraction, material transportation inside cells, genetic material (DNA) replication, to cell division, etc., traced to the molecular level, are all derived from protein macromolecules with motor functions As a result of doing work, they are called molecular motors or motor proteins. In the current research on motor proteins, kinesin is the most widely studied. Kinesin uses ATP as its energy source and moves along microtubules. In view of the robustness and high efficiency of this motor molecular movement, kinesin has become an ideal driving component when constructing active biomimetic systems on the micron or nanoscale scale, attracting more and more scientists' interest. Recent studies have successfully applied them to some in vitro artific...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/68
CPCC07K14/47
Inventor 李峻柏贾怡李洁龄冯熙云董伟光
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com