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Preparation method of mesoporous silicon nanoparticles with antibody-mediated optomagnetic dual-mode

A nanoparticle, dual-modal technology, applied in the field of biomedicine, to achieve the effect of simple method, good biocompatibility, and simple step conditions

Inactive Publication Date: 2016-06-15
XIDIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

As for the use of nano-ferric iron tetroxide as the core, coated with silica, labeled with fluorescein isothiocyanate, aminated by surface modification of 3-aminopropyltriethoxysilane, and then modified by carboxyaminotransfer Linked with lactobionic acid, and finally by activating the carboxyl group and the prostate cancer stem cell antigen antibody (PSCA), to obtain the patent of the dual-modal mesoporous silicon nanoparticle with active targeting function, there is no similar report

Method used

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  • Preparation method of mesoporous silicon nanoparticles with antibody-mediated optomagnetic dual-mode
  • Preparation method of mesoporous silicon nanoparticles with antibody-mediated optomagnetic dual-mode
  • Preparation method of mesoporous silicon nanoparticles with antibody-mediated optomagnetic dual-mode

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preparation example Construction

[0037] Such as figure 1 As shown, this method is a preparation method of antibody-mediated optomagnetic dual-mode mesoporous silicon nanoparticles, comprising the following steps:

[0038] (1) Add 0.01g~10g0.01mmol~10mmol of fluorescein isothiocyanate and 0.10g~10g0.1mmol~10mmol of 3-aminopropyltriethoxysilane into 10ml~100ml of ethanol, in 10 Under the temperature of ℃~60℃, react in the dark with 0.1mol~5mol of nitrogen gas for 10h~28h to obtain 3-aminopropyltriethoxysilane modified by fluorescein isothiocyanate, which is recorded as FITC-APTES;

[0039] (2) Disperse 0.1ml~10ml of citric acid-protected ferric oxide nanoparticle aqueous solution (0.001g / ml~10g / ml) in a mixture of 40ml~130ml ethanol and water, ultrasonicate for 20min~120min, add ammonia water 0.1ml~ 10ml, the concentration is 0.1mol / l~20mol / l as a catalyst, at 10℃~60℃, stir vigorously for 10min~6.5h, the stirring speed is 60r / s, add 0.01ml~5ml tetraethoxysilane, in At 10°C-60°C, react for 2h-24h, add 0.1ml-20...

Embodiment 1

[0053]Add 0.01g0.01mmol of FITC and 0.10g0.1mmol of APTES to 10ml of ethanol, at 10°C, avoid light and pass 0.1mol of nitrogen, and react for 10h to obtain FITC-APTES; Nanoparticle aqueous solution 0.1ml0.001g / ml is dispersed in 10ml ethanol and water mixture, the mixing ratio is 5:2, ultrasonic for 20min, add 0.1mol / l ammonia water 0.1ml as a catalyst, stir vigorously at 10℃ for 10min , Stirring speed 60r / s, add 0.01ml TEOS, react at 10℃ for 2h, add FITC-APTES0.1ml, continue stirring for 2h, add 0.01ml TEOS dropwise within 1h, avoid light and stir vigorously for 10h, The stirring speed is 60r / s, and the nanoparticles of functional groups are obtained, which are recorded as FMNPs. Use 1ml of water for the first centrifugal washing, 1ml of ethanol for the second centrifugal washing, 1ml of water for the third centrifugal washing three times, and dry For storage, disperse 0.01g of bifunctional nanoparticle FMNPs in a mixture of 10ml of ethanol and water, the mixing ratio is 5:1,...

Embodiment 2

[0055] Add 0.1g0.1mmol of FITC and 0.20g0.5mmol of APTES to 20ml of ethanol, at 20°C, avoid light and pass 0.5mol of nitrogen, react for 12h, and obtain FITC-APTES; Nanoparticle aqueous solution 0.2ml0.01g / ml is dispersed in a mixture of 20ml ethanol and water, the mixing ratio is 5:2, sonication for 30min, adding 0.5ml of 1mol / l ammonia water as a catalyst, stirring vigorously for 30min at 20°C, Stirring speed 60r / s, add 0.1ml of TEOS, react at 20°C for 5h, add FITC-APTES0.5ml, continue stirring for 5h, add 0.1ml of TEOS dropwise within 2h, avoid light and vigorously stir for 12h, stir The speed is 60r / s, and the nanoparticles of functional groups are obtained, which are recorded as FMNPs. Use 2ml of water for the first centrifugal washing, 2ml of ethanol for the second centrifugal washing, 2ml of water for the third centrifugal washing three times, and dry storage , disperse 0.1g of bifunctional nanoparticle FMNPs in a mixture of 20ml of ethanol and water, the mixing ratio i...

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Abstract

The invention belongs to the field of nanocomposite materials, and discloses a method for preparing mesoporous silicon nano-particles with antibody-mediated optomagnetic dual-mode, which uses nanometer ferric oxide as the core, uses silicon dioxide to wrap, and isothiocyanate Acid fluorescein labeling, amination by surface modification of 3-aminopropyltriethoxysilane, and then grafted lactobionic acid by carboxyl amino cross-linking, and finally by activating the carboxyl group and prostate cancer stem cell antigen antibody (PSCA). The method of the invention is simple and easy to operate, and the obtained nano particles have the advantages of active targeting, good biocompatibility, less toxic and side effects, good monodispersity and stability, stable magnetic properties and fluorescent properties, and the like.

Description

Technical field: [0001] The invention relates to a method for preparing mesoporous silicon nanoparticles with antibody-mediated optomagnetic dual modes, and belongs to the technical field of biomedicine. Background technique: [0002] Fe304 nanoparticles are a typical magnetic material. Since Fe304 nanoparticles have both the quantum size effect and surface effect of nanoparticles and the unique magnetic responsiveness of magnetic materials, it has special properties that conventional particles do not have: (1) Fe304 nanoparticles have excellent magnetic sensitivity. Magnetic Fe304 nanoparticles have strong magnetism, can obtain strong magnetic induction in a very weak external magnetic field, and can move directionally; (2) have superparamagnetism in a certain size distribution range. When the particle size of magnetic nanoparticles is less than 16nm, its anisotropy is reduced to be comparable to the thermal kinetic energy, and the direction of easy magnetization changes i...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/16A61K49/08A61K49/00A61K47/42A61K47/48A61K47/04
Inventor 詹勇华李英超韩青林李智敏梁继民田捷
Owner XIDIAN UNIV
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