Preparation method and application of electrochemical biosensor based on three-dimensional nano-porous gold-modified boron-doped diamond electrode

A technology of diamond electrodes and biosensors, applied in the direction of electrochemical variables of materials, nanotechnology for sensing, scientific instruments, etc., can solve the problems of low electrocatalytic activity, difficult surface reconstruction, selectivity and Poor sensitivity and other issues, to achieve the effect of improving electrocatalytic activity and sensitivity, promoting electron transfer, and improving sensitivity

Inactive Publication Date: 2014-09-10
HENAN UNIVERSITY OF TECHNOLOGY
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  • Abstract
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Problems solved by technology

[0004] The technical problem to be solved in the present invention is a new type of electrode material, boron-doped diamond thin film electrode has low electrocatalytic activity, difficult surface reconstruction, poor selectivity and sensitivity, and provides a method to improve the electrocatalytic activity and sensitivity of the electrode, which will help Preparation and Application of Immobilized Acetylcholinesterase Electrochemical Biosensor Based on Three-dimensional Nanoporous Gold Modified Boron-doped Diamond Electrode

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  • Preparation method and application of electrochemical biosensor based on three-dimensional nano-porous gold-modified boron-doped diamond electrode
  • Preparation method and application of electrochemical biosensor based on three-dimensional nano-porous gold-modified boron-doped diamond electrode
  • Preparation method and application of electrochemical biosensor based on three-dimensional nano-porous gold-modified boron-doped diamond electrode

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preparation example Construction

[0017] Such as Figure 1 to Figure 6 Shown: a method for preparing an electrochemical biosensor based on three-dimensional nanoporous gold-modified boron-doped diamond electrodes, which includes the following steps: ①Using template method and chemical growth method to prepare three-dimensional nanoporous gold on boron-doped diamond electrodes and Modify the boron-doped diamond electrode to obtain a three-dimensional nanoporous gold-modified boron-doped diamond electrode; Electrochemical biosensors with heterogeneous diamond electrodes.

[0018] The preparation of the three-dimensional nanoporous gold-modified boron-doped diamond electrode by the template method and the chemical growth method comprises the following steps: a. preparing polystyrene spheres with good monodispersity and a particle size of 250-800 nm as a template; b. Pre-deposit a thin layer of gold (Au) nano-dots with a thickness of 4-6nm on the doped diamond electrode (BDD) as the seeds of gold grown by the che...

Embodiment 1

[0023] First, prepare 250nm polystyrene spheres (PS) with good monodispersity: add 10 mL of 3.87% monomeric styrene, 1 mL of 10.17% methacrylic acid, and 225 mL of twice distilled water into a three-necked flask. Introduce nitrogen to remove the oxygen in it, set the speed at about 300rpm, and start heating; heat up to 70°C, and add 0.3239g of initiator potassium persulfate after the temperature is stable; after adding the initiator for 13 hours, stop heating, and the reaction is complete , That is, a polystyrene ball (PS) with a diameter of about 250nm.

[0024]A thin layer of gold (Au) nanodots with a thickness of 5nm was pre-deposited on the boron-doped diamond electrode as the seed of gold grown by the chemical growth method. Based on the characteristics of self-assembly of nanoparticles with good monodispersity, a boron-doped diamond electrode deposited with gold nanodots is used as a substrate, and the substrate is slowly pulled out of the suspension by using a vertical ...

Embodiment 2

[0028] Prepare 300nm polystyrene spheres (PS) with good monodispersity, and the preparation steps are similar to Example 1.

[0029] A thin layer of gold (Au) nanodots with a thickness of 4nm was pre-deposited on the boron-doped diamond electrode as the seed of gold grown by chemical growth method. Based on the characteristics of self-assembly of nanoparticles with good monodispersity, the BDD electrode deposited with gold nano-dots is used as the substrate, and the substrate is slowly pulled from the suspension to obtain the film thickness by using the vertical pulling method. Uniform polystyrene ball template; sintering at 70-100°C for 4h to fix the polystyrene ball template; use the chemical growth method to vertically immerse the boron-doped diamond electrode assembled with the polystyrene ball template into chloroauric acid (1wt%, 0.3 mL) and hydroxylamine hydrochloride (0.04mol / L, 1mL) in 20mL aqueous solution, oscillating for 10 minutes to grow gold, and then immersing ...

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Abstract

The invention discloses a preparation method and application of an electrochemical biosensor based on a three-dimensional nano-porous gold-modified boron-doped diamond electrode, and in particular relates to a preparation method of a boron-doped diamond electrode, a fixation method of acetylcholin esterase and application of acetylcholin esterase in organophosphorus pesticide residue detection for solving the technical problem that the boron doped diamond membrane electrode has low electrocatalytic activity, difficulty in surface reengineering and poor selectivity and sensitivity. The method comprises the following steps: 1, preparing three-dimensional nano-porous gold on the boron doped diamond electrode and modifying the boron doped diamond electrode; 2, fixing acetylcholin esterase on the three-dimensional nano-porous gold modified boron doped diamond electrode modified by the step 1 to construct the electrochemical biosensor. The electrochemical biosensor adopting the technical scheme is used for fixing the acetylcholin esterase and is used for electrochemical detection of organophosphorus pesticide residues, and the method can be used for increasing the specific surface, accelerating electron transfer, improving the electrocatalytic activity and improving the detection sensitivity.

Description

technical field [0001] The invention relates to an electrochemical biosensor, in particular to the preparation of a three-dimensional nanoporous gold-modified boron-doped diamond electrode, the immobilization of acetylcholinesterase and its application in the detection of organophosphorus pesticide residues. Background technique [0002] Due to the widespread use of organophosphorus pesticides, it has caused serious pollution to the environment and endangered human health. Although traditional detection methods such as chromatography have the advantages of certainty, quantification, accuracy, and high sensitivity, they require professional operators, expensive instruments, and complicated sample pretreatment processes, so rapid detection cannot be achieved. Electrochemical detection method has been widely studied as a rapid detection method in the field of organophosphorus pesticide detection due to its advantages of fast response, simple operation, high sensitivity, and on-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/333G01N27/26B82Y15/00
Inventor 卫敏谢岩黎金华丽
Owner HENAN UNIVERSITY OF TECHNOLOGY
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