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Method for separating icariine from herba epimedii

A technology of icariin and epimedium, applied in chemical instruments and methods, preparation of sugar derivatives, sugar derivatives, etc., can solve the problems that the microscopic mechanism of action needs to be further studied, and the active ingredients of epimedium are not deep enough, etc. Achieve good development prospects, treat cardiovascular and cerebrovascular diseases, enhance immune function and immune regulation effects

Inactive Publication Date: 2014-09-24
西安岳达生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current development research is still in its infancy, and the research on the active ingredients of Epimedium is not deep enough, and the microscopic mechanism of action of the pharmacological effects observed on the macroscopic level needs to be further studied

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  • Method for separating icariine from herba epimedii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The method for isolating icariin from epimedium, comprises the following steps:

[0024] 1) 70kg of Epimedium, icariin content 1.0%, placed in an extraction tank, 780L of 20% ethanol for the first extraction, 130L of 20% ethanol for the second time, each time for 2 hours, boiled for extraction, the temperature is about 90 degrees, combined The filtrate was concentrated until the density reached 1.12 to obtain 56Kg of extract;

[0025] 2) After adding 100Kg of ethanol for alcohol precipitation, remove Epimedium polysaccharide, concentrate the filtrate again to a density of 1.12 or more to obtain 36Kg of extract, extract the obtained extract with 500L of ethyl acetate in 6 times, until the water phase is detected by TLC less than icariin;

[0026] 3) All the ethyl acetate extracts were combined, dehydrated with anhydrous sodium sulfate, and concentrated to dryness to obtain 1.8Kg of a brown solid, which was added to 5L of anhydrous methanol and boiled to dissolve;

[00...

Embodiment 2

[0029] The method for isolating icariin from epimedium, comprises the following steps:

[0030] 1) 80kg of Epimedium, icariin content 1.0%, placed in an extraction tank, 1100L 20% ethanol for the first extraction, 1200L 20% ethanol for the second time, each time 1h, boiled for extraction, the temperature is about 90 degrees, combined The filtrate was concentrated until the density reached 1.12 to obtain 87Kg of extract;

[0031] 2) After adding 200Kg of ethanol for alcohol precipitation, remove Epimedium polysaccharide, concentrate the filtrate again to a density of 1.12 or more, and obtain 63Kg of extract, extract the obtained extract with 300L of ethyl acetate in 6 times, until the water phase is detected by TLC less than icariin;

[0032] 3) All the ethyl acetate extracts were combined, dehydrated with anhydrous sodium sulfate, and then concentrated to dryness to obtain 2.3Kg of a brown solid, which was added to 5L of anhydrous methanol and boiled to dissolve;

[0033] 4)...

Embodiment 3

[0035] The method for isolating icariin from epimedium, comprises the following steps:

[0036] 1) 60kg of Epimedium, icariin content 1.0%, placed in an extraction tank, 800L 20% ethanol for the first extraction, 900L 20% ethanol for the second time, each time for 3 hours, boiled for extraction, the temperature is about 90 degrees, combined The filtrate was concentrated until the density reached 1.12 to obtain 62Kg of extract;

[0037] 2) After adding 200Kg of ethanol for alcohol precipitation, remove Epimedium polysaccharide, concentrate the filtrate again to a density of 1.12 or more to obtain 46Kg of extract, extract the obtained extract with 300L of ethyl acetate in 6 times, until the water phase is detected by TLC less than icariin;

[0038] 3) All the ethyl acetate extracts were combined, dehydrated with anhydrous sodium sulfate, and concentrated to dryness to obtain 0.9Kg of a brown solid, which was added to 5L of anhydrous methanol and boiled to dissolve;

[0039] 4)...

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Abstract

The invention discloses a method for separating icariine from herba epimedii, and belongs to the technical field of biological medicine. The method comprises the following steps: 1) respectively extracting herba epimedii twice by using ethanol; 2) merging filtrates, and concentrating to obtain extractum; 3) adding ethanol to remove epimedium polysaccharide, and extracting for six times by using ethyl acetate; and 4) merging all ethyl acetate extracting solutions, and concentrating, filtering and baking after dewatering by using anhydrous sodium sulfate, so as to obtain the icariine. The extracting method disclosed by the invention is concise in process, wide in raw material herba epimedii source, low in cost, and environment-friendly in the extracting preparation process. A broad market is opened up for development of novel icariine healthcare products and drugs.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a method for separating icariin from epimedium. Background technique [0002] Icariin is an 8-prenyl flavonoid glycoside compound derived from the plants of the Berberidaceae (Berberidaceae) Epimedium (Epimedium), studies have shown that it can improve the function of the cardiovascular and cerebrovascular systems, enhance the body's immunity And promote physiological activities such as DNA synthesis. Pharmacological effects: Icariin can increase the blood flow of cardiovascular and cerebrovascular, promote hematopoietic function, immune function and bone metabolism, and has the functions of nourishing kidney and strengthening yang, anti-aging and anti-tumor. 1. The effect on endocrine: Icariin can promote sexual function because of the hypersecretion of semen and the filling of seminal vesicles, which stimulates sensory nerves and indirectly stimulates sexual de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08
Inventor 张天
Owner 西安岳达生物科技股份有限公司