Method for preparing high-purity sulforaphane

A sulforaphane, high-purity technology, applied in the direction of fermentation, etc., can solve the problems of unregulated, short half-life of free enzyme, and low production rate of sulforaphane

Inactive Publication Date: 2014-09-24
重庆海巨农业发展有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main problems in this method are: 1. The seeds are not degreased. Due to the blocking of oil, the sinaprin and glucuronidase in the broccoli seeds are difficult to effectively contact, resulting in a low production rate of sulforaphane; 2. Immobilization of the extracted myrosinase, the free enzyme has a short half-life and is easily inactivated; 3. The organic solvent used - acetone has a strong ability to denature the enzyme protein, resulting in a large amount of denatura

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for preparing high-purity sulforaphane, the specific method steps are as follows:

[0030] 1. Preparation of defatted radish seed powder

[0031] Take air-dried radish seeds with a moisture content lower than 4%, first crush them with a crusher, and then sieve them with a 20-mesh stainless steel sieve, and collect the sieved and unsifted ones respectively. For the unscreened radish seed powder, return to the crusher for crushing again; for the sieved radish seed powder, put it in the extraction kettle of the supercritical carbon dioxide extractor, and extract it at a temperature of 30°C and a pressure of 25MPa 1h, then pump the supercritical carbon dioxide loaded with radish seed oil into the separation kettle, reduce the pressure to 3MPa, gasify the supercritical carbon dioxide, and separate it from the radish seed oil. Collect the gasified carbon dioxide, the crude product of decarbonated radish seed oil and the defatted radish seed powder, and freeze the co...

Embodiment 2

[0045] A method for preparing high-purity sulforaphane, the specific method steps are as follows:

[0046] 1. Preparation of defatted radish seed powder

[0047] With embodiment 1, wherein: 40 mesh stainless steel screens are used to sieve the crushed radish seeds. Extraction was carried out for 1.5 h under the conditions of temperature at 33° C. and pressure at 28 MPa. Reduce the pressure to 4MPa to vaporize the supercritical carbon dioxide and separate it from the radish seed oil. The collected vaporized carbon dioxide was re-frozen to 3°C and re-pressurized to 28Mpa.

[0048] 2. Preparation of glucosinolate extract

[0049] Same as Example 1, wherein: the first extraction was carried out at a temperature of 73° C. for 50 minutes, and the ratio of defatted radish seed powder mass (g) to methanol volume (mL) was 1:7. The concentration vacuum is 0.08MPa and the temperature is 43°C. The ratio of the mass (g) of the paste concentrate to the volume (mL) of distilled water is...

Embodiment 3

[0061] A method for preparing high-purity sulforaphane, the specific method steps are as follows:

[0062] 1. Preparation of defatted radish seed powder

[0063] With embodiment 1, wherein: the radish seeds after sieving and crushing are carried out with 60 mesh stainless steel screens. The extraction was carried out for 2 hours under the conditions of temperature at 35° C. and pressure at 30 MPa. Reduce the pressure to 5MPa to vaporize the supercritical carbon dioxide and separate it from the radish seed oil. The collected vaporized carbon dioxide was re-frozen to 5°C and re-pressurized to 30Mpa.

[0064] 2. Preparation of glucosinolate extract

[0065] Same as Example 1, wherein: the first extraction was carried out at a temperature of 75° C. for 60 minutes, and the ratio of defatted radish seed powder mass (g) to methanol volume (mL) was 1:9. The concentration vacuum is 0.09MPa and the temperature is 45°C. The ratio of the mass (g) of the paste concentrate to the volum...

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PUM

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Abstract

The invention provides a method for preparing high-purity sulforaphane and in particular relates to a method for extracting the sulforaphane from radish seeds. The method is used for preparing products having the purity of more than 98% from air-dried radish seeds as the raw material and through the steps of cracking, degreasing, hydrolysis, initial separation by use of resin, reverse osmosis concentration, preparation separation and freeze-drying. The method is simple and convenient to operate, simple in equipment, mild in reaction conditions, energy-saving, low in production cost, and convenient to popularize and utilize. The used methanol waste liquid is recovered, the used resin is regenerated and impurities generated can be recycled, and therefore, the production is safe, green and pollution-free, and the production method is capable of saving energy and reducing emission. The sulforaphane product prepared by the method can be widely applied to the industries such as medicines and health care products.

Description

1. Technical field [0001] The invention belongs to the technical field of separation and preparation of sulforaphane, in particular to a method for extracting sulforaphane from radish seeds. 2. Background technology [0002] Glucosinolate is a kind of glucosinolate in cruciferous vegetables. Under the catalysis of glucosinosidase or acid, glucosinolate is hydrolyzed to generate isothiocyanates such as sulforaphane and glucose. Scientific research has found that the chemical name of sulforaphane is 1-isothiocyanate-4-methanesulfonyl butane or 1-isothiocyanate-4-methanesulfonyl-(2-ene)butane, which is by far the One of the strongest anti-cancer ingredients found in vegetables, it can induce the body to produce type II detoxification enzymes, which can prevent carcinogens from destroying genetic factors in healthy cells, so 1-isosulfur is isolated and purified from cruciferous vegetables Cyanate-4-methanesulfonylbutane or 1-isothiocyanate-4-methanesulfonyl-(2-ene)butane has im...

Claims

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Application Information

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IPC IPC(8): C12P13/00
Inventor 刘庆海万玉萍颜李秀冯琳周小华
Owner 重庆海巨农业发展有限公司
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