Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

A light-controllable ATP biosynthesis system and preparation method thereof

A biosynthesis, system technology, applied in the direction of fermentation, etc.

Active Publication Date: 2016-06-15
INST OF CHEM CHINESE ACAD OF SCI
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report of using photosystem II protein as proton source to promote ATP synthesis

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A light-controllable ATP biosynthesis system and preparation method thereof
  • A light-controllable ATP biosynthesis system and preparation method thereof
  • A light-controllable ATP biosynthesis system and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1, preparation photosystem II / ATP synthase assembly system

[0043] (1) Preparation of photosystem II gel microspheres by co-precipitation

[0044](a) Configure 0.33M calcium chloride and sodium carbonate solutions with secondary water, mix equal volumes, and add 20 mmol MES (2-(N-morpholine) ethanesulfonic acid) buffer solution (pH value is 6) 0.5mL of 20mg / mL BSA (Bovine Serum Albumin) solution and 0.5mL of 2mg / mL Photosystem II solution were mixed rapidly and uniformly stirred for 40s, left to stand for 2min, and centrifuged to collect calcium carbonate microspheres co-precipitated by BSA and Photosystem II.

[0045] (b) Disperse calcium carbonate microspheres in MES (2-(N-morpholine)ethanesulfonic acid) solution containing 0.025wt% GA (glutaraldehyde), cross-link for 3 hours, wash by centrifugation, and remove the template with 0.1 MEDTA Calcium carbonate particles; continue to disperse in MES solution containing 0.3mgchl / mL Photosystem II, crosslink for...

Embodiment 2

[0055] Embodiment 2, preparation photosystem II / ATP synthase assembly system

[0056] (1) Preparation of photosystem II microcapsules

[0057] Manganese carbonate particles with a particle size of 5 microns were dispersed in a solution containing 0.2M sodium chloride and 2 mg / mL polyacrylamide for 30 minutes, then centrifuged, washed thoroughly, and then dispersed in a solution containing 0.025 wt% GA. React for 3 hours, wash thoroughly, then disperse into 40mmol MES buffer solution (pH=6) containing 0.5mg / mL photosystem II and 2mg / mL BSA, shake and adsorb for 4h, the aldehyde group on GA reacts with the amino group of the protein, so that the protein After being fixed on the surface of the particle, an assembly cycle is completed, and the operations of adsorbing GA and photosystem II are repeated in sequence until the required number of layers is reached. The template manganese carbonate particles were removed with 0.1 MEDTA to obtain BSA / PSII / GA covalently cross-linked micr...

Embodiment 3

[0062] Embodiment 3, preparation photosystem II / ATP synthase assembly system

[0063] (1) Preparation of photosystem II microspheres

[0064] Disperse silica particles with a particle size of 500nm in a solution of 1mg / mL polyacrylamide containing 0.2M sodium chloride and absorb for 30min, then centrifuge, wash thoroughly, and then disperse in a solution containing 0.025wt% GA, React for 3 hours, wash thoroughly, then disperse into 40mmol MES buffer solution (pH=6) containing 0.5mg / mL Photosystem II and 2mg / mL BSA, shake and adsorb for 4h, and complete an assembly cycle, repeat the adsorption of GA and Photosystem II in sequence operation until the desired number of layers. Nano-silica particles are biocompatible, have no effect on protons, and do not need to remove nuclei. Microspheres with a diameter of about 500nm were obtained. The beads were dispersed in buffer and stored at 4°C.

[0065] (2) The preparation of proteoliposomes containing ATP synthase was the same as s...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a light-controllable ATP biosynthesis system and a preparation method thereof. The method comprises the following steps: (1) preparing photosystem II microspheres or photosystem II capsules; (2) dispersing the photosystem II microspheres or the photosystem II capsules into a 2- Adsorb in (N-morpholine) ethanesulfonic acid buffer; then continue to disperse in 2-(N-morpholine) ethanesulfonic acid buffer containing photosystem II for adsorption; (3) will undergo step (2) The treated photosystem II microspheres or photosystem II capsules are dispersed into the proteoliposome solution containing ATP synthase for adsorption, and the light-controllable ATP biosynthesis system is obtained. In the present invention, photosystem II is immobilized on the surface of 3D (micro-nanospheres and micro-nanocapsules), increasing the area of ​​action and applicability; the size, structure and function can be adjusted: it can be arbitrarily changed by selecting different synthesis methods according to actual needs The size, structure and encapsulation of additional functional capsule wall components of the sphere or capsule.

Description

technical field [0001] The invention relates to an ATP synthesis system and a preparation method thereof, in particular to a light-controllable ATP biosynthesis system and a preparation method thereof. Background technique [0002] ATP is the "energy currency" in organisms and plays an important role in energy metabolism. It provides energy for a variety of energy-consuming processes in cells and participates in the regulation of various biochemical processes, such as nerve conduction, metabolism, muscle contraction, bioluminescence, and material transport. Due to its diverse functions, if the controllable synthesis of ATP in cells can be simulated at the subcellular level, it will be able to achieve fine control and research on various biological processes. [0003] ATP is synthesized by ATP synthase in living organisms. Using ATP synthase to construct the assembly structure of the biomimetic system can realize the fine control of ATP synthesis on the artificial carrier, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/32
Inventor 李峻柏冯熙云蔡鹏贾怡费进波董伟光李洁龄
Owner INST OF CHEM CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com