Method for removing calcium carbonate scale with microbial extracellular carbonic anhydrase
A technology of carbonic anhydrase and calcium carbonate scale, applied in chemical instruments and methods, biological water/sewage treatment, complex/solubilizer treatment, etc., can solve problems such as water eutrophication and environmental pollution
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Embodiment 1
[0029] Embodiment 1 The inventive method removes calcium carbonate scale
[0030] 1) Bacteria preparation: Take an appropriate amount of cooling water from the circulating water system and spread it evenly on the 4 On the beef extract peptone agar plate, culture the plate at 34-37°C for 24 hours, pick out the well-grown colony and purify it by streaking method.
[0031] 2) Expansion culture: Inoculate the microorganism (Bacillus subtilis) purified in step 1) into LB liquid medium and carry out expansion culture to the stationary phase, the culture temperature is 30±1°C, and the shaker speed is 120r / min.
[0032] 3) Extraction of crude carbonic anhydrase enzyme liquid: centrifuge the cultured bacterial liquid in 2) at 4° C., 5000 r / min for 10 min to remove cells. Add appropriate amount (NH 4 ) 2 SO 4 Make the solution saturated to 70%, place it at 4°C for salting out for 12 hours, then centrifuge at 8000r / min for 30 minutes at 4°C to obtain the protein, and dissolve the pro...
Embodiment 2
[0036] Embodiment 2 The inventive method removes calcium carbonate scale
[0037] 1) Bacteria preparation: Take an appropriate amount of cooling water from the circulating water system and spread it evenly on the 4 On the beef extract peptone agar plate, culture the plate at 34-37°C for 24 hours, pick out the well-grown colony and purify it by streaking method.
[0038] 2) Expansion culture: Inoculate the microorganism (Bacillus subtilis) purified in step 1) into LB liquid medium and carry out expansion culture to the stationary phase, the culture temperature is 30±1°C, and the shaker speed is 120r / min.
[0039] 3) Extraction of crude carbonic anhydrase enzyme liquid: centrifuge the cultured bacterial liquid in 2) at 4° C., 5000 r / min for 10 min to remove cells. Add appropriate amount (NH 4 ) 2 SO 4 Make the solution saturated to 70%, place it at 4°C for salting out for 12 hours, then centrifuge at 8000r / min for 30 minutes at 4°C to obtain the protein, and dissolve the pro...
Embodiment 3
[0042] Example 3 Treatment of scale formed in the pipes of the circulating water system of a chemical plant
[0043] Before the treatment, the system had serious fouling, and the blocky scale closely adhered to the inner wall of the system, resulting in blockage of pipes and reduced heat transfer efficiency. Expand the culture of microorganisms that secrete extracellular carbonic anhydrase to an OD600nm value of 0.8 or more, put the crudely extracted carbonic anhydrase into the circulating water system of the factory, and after 10 days of normal operation, it is found that the massive scale on the inner wall of the system is loose With the scour of the circulating water flow and falling into the sump, the phenomenon of pipe blockage has been improved, and the heat exchange efficiency of the system has also been improved. The scale deposited in the sump was collected and found to be in powder form. The test results showed that its main components were carbonates such as calcium...
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Abstract
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