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Application of schisandrol B in preparation of medicine for preventing and treating cholestatic liver disease

A technology of schisandra alcohol B and cholestasis, which is applied in the field of medicine to achieve the effects of reducing bile acid levels, reducing liver damage, and reducing the degree of liver tissue necrosis

Inactive Publication Date: 2014-10-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, whether Schisandrin B can prevent and treat cholestasis has not been reported yet.

Method used

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  • Application of schisandrol B in preparation of medicine for preventing and treating cholestatic liver disease
  • Application of schisandrol B in preparation of medicine for preventing and treating cholestatic liver disease
  • Application of schisandrol B in preparation of medicine for preventing and treating cholestatic liver disease

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Example 1 Treatment of the mouse intrahepatic cholestasis model caused by excess lithocholic acid

[0054] 1. Materials and methods

[0055] (1) Main instruments:

[0056] 5417-R low-temperature high-speed centrifuge (Eppendorf, Germany); full-wavelength microplate reader (Thermo, USA); Mini-protein3 electrophoresis system (Bio-Rad, USA); Mini Trans-Blot transfer system (Bio-Rad, USA) ; ImageQuant LAS 4000 Exposure Imager (General Electric, USA); Gradient PCR (Eppendorf, Germany); 7500 Real Time PCR System (Applied Biosystems, USA).

[0057] (2) Drugs and reagents

[0058] Schisandra Alcohol B (purity ≥98%, Shanghai Ronghe Pharmaceutical Technology Development Co., Ltd.); lithocholic acid (purity ≥98%, Shanghai Aladdin Reagent Co., Ltd.), sodium carboxymethylcellulose (CMC, purity ≥98%, American Sigma Company); corn oil (pharmaceutical grade, Shanghai Aladdin Reagent Co., Ltd.); pregnaneenone 16-carbonitrile (PCN, purity ≥ 98%, American Sigma Company); ursodeoxycho...

Embodiment 2

[0097] Example 2 Treatment of extrahepatic cholestasis model in mice caused by bile duct ligation

[0098] 1. Materials and methods

[0099] (1) The main instruments are the same as in Example 1.

[0100] (2) Drugs and reagents: ursodeoxycholic acid (UDCA, Shanghai Aladdin Reagent Co., Ltd.); the rest are the same as in Example 1.

[0101] 2. Experimental animals and dosing regimen

[0102] C57BL / 6 mice, male, 8-9 weeks old, were provided by the Experimental Animal Center of Sun Yat-sen University (University Town, Guangzhou City), license number SCXK (Guangdong) 2011-0029, and normal mice were fed with maintenance feed.

[0103] Schisandrin B (200 mg / kg) was ultrasonically dispersed in pre-autoclaved 0.5% (w / w) CMC; ursodeoxycholic acid (25 mg / kg) was dissolved in saline.

[0104] Mice were randomly divided into 5 groups, 6 to 8 in each group, the groups were sham operation blank control group, sham operation + schisandrin B group, bile duct ligation blank control group,...

Embodiment 3

[0121] Example 3 Treatment of Schisandra Alcohol B to Cholesterol Stones, Hyperlipoproteinemia, etc.

[0122] The present invention has also preliminarily studied the impact and therapeutic effect of schisandra alcohol on cholesterol gallstones and hyperlipoproteinemia. The prevention and treatment effect is obvious.

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Abstract

The invention discloses application of schisandrol B in preparation of a medicine for preventing and treating cholestatic liver disease. By giving an excess amount of lithocholic acid, a mice intrahepatic cholestasis model is made, or a mice extrahepatic cholestasis model is made by bile duct ligation, then the mice intrahepatic cholestasis model and the mice extrahepatic cholestasis model are respectively collaboratively given the schisandrol, liver injury can be effectively alleviated, the bile acid level is reduced, glutamic-pyruvic transaminase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activity are decreased significantly, liver tissue necrosis degree is significantly alleviated, at the same time, total bile acid and total bilirubin content in serum are significantly reduced, and the total bile acid and total bilirubin content discharged with urine are increased. The results show that the schisandrol B can prevent and treat cholestatic liver injury induced by lithocholic acid and bile duct ligation, and prove that the effect mechanism may be involved in regulation of CYP3A11, UGT1A1 and OATP1A4 mediated bile acid metabolism and excretion.

Description

technical field [0001] The invention belongs to the field of medicine. More specifically, it relates to the application of schisandra alcohol B in the preparation of drugs for the prevention and treatment of cholestatic liver diseases. Background technique [0002] Cholestasis is a common clinical symptom of a group of diseases caused by bile production disorder or / and bile excretion disorder, which causes the bile components excreted by liver cells to flow back into the blood, resulting in retention, and may eventually develop into a fatal condition. Liver diseases such as primary biliary cirrhosis and sclerosing cholangitis. If only bile acid in the blood is increased but bilirubin is normal, it is called cholestasis or cholestasis, and if both bile acid and bilirubin in the blood are increased, it is called cholestatic jaundice. According to the location of cholestasis, it is usually divided into two categories: intrahepatic cholestasis and extrahepatic cholestasis base...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/36A61P1/16A61P3/06
Inventor 黄民毕惠嫦曾行陈攀范晓梅姜伊鸣
Owner SUN YAT SEN UNIV
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