Multichannel array type DNA (Deoxyribose Nucleic Acid) sequencing system and sequencing method thereof

Abstract

The invention relates to a nanopore DNA (Deoxyribose Nucleic Acid) sequencing sensor for detecting DNA deoxyribonucleotide base sequence. The sensor comprises a current detection unit, a molybdenum disulfide field-effect tube, an atomic power microscope feeding system and an array unit, wherein DNA is bonded on an atomic power microscope probe by a chemical modification method; by virtue of a feeding control system of the atomic power microscope, a speed for driving DNA to go in and out of the nanopore is controlled to be 1nanometer per second; the speed fully meets the requirement of bandwidth for detecting DNA sequencing current signal; the nanopore is formed in a molybdenum disulfide nanoribbon in the process of penetrating through the pore of DNA; the molybdenum disulfide nanoribbon plays the role of the field-effect tube in the process of detecting the current signal and can be used for amplifying a signal for showing the DNA penetrating through the hole in real time and effectively increasing a signal to noise ratio; in addition, the method for arraying the atomic power microscope probe corresponding to the molybdenum disulfide field-effect tube can be used for carrying out multi-channel parallel real-time sequencing on the DNA to be detected parallel at the same time, so that the time cost is greatly reduced.

Description

technical field [0001] The invention relates to a nanopore DNA sequencing system and a method thereof, in particular to a multi-channel array DNA sequencing system integrating an atomic force microscope and a molybdenum disulfide field effect tube and a sequencing method thereof. Background technique [0002] Nanopore single-molecule sensors have been widely used in the detection of single molecules due to their low-cost and high-throughput characteristics. The basic working principle of the nanopore single-molecule sensor is based on the Coote counter. When the charged single molecule electrophoresis passes through the nanopore under the condition of applied voltage, because of the physical occupation of the single molecule itself in the hole and the distance between it and the nanopore wall The strong interaction between them makes the ionic current of the through hole modulated, and by detecting the amplitude and blocking time of the modulated ionic current, the type of s...

AI Technical Summary

Problems solved by technology

Reducing the driving voltage, lowering the system temperature, increasing the viscosity of the solution, and replacing the inorganic salt solution with an organic salt solution can all reduce the passing speed of DNA in an appropriate amount, but it is still far from reducing to the speed required for DNA sequencing; The speed of DNA polymerization is about tens of milliseconds per base, so the use of polymerase-driven DNA can meet the requirements of DNA sequencing. However, the operation of polymerase-driven DNA is complex and demanding on the environment, which violates the need for modification of the third-generation DNA. The original intention of simplicity and low cost; the method of mechanical manipulation has become one of the effective methods for manipulating DNA through holes because it can control the direction of movement and speed of DNA through holes. DNA manipulation methods such as optical tweezers and magnetic tweezers have been studied However, due to the complex operation of optical tweezers and magnetic tweezers and the immature technology, it will take a long time to apply to the active control of DNA via holes.

Images