In situ detection of abundance and diversity of marine bacteria based on laser-induced fluorescence

Abstract

A laser-induced fluorescence-based in situ detection method for the abundance and diversity of marine bacteria with a wide range of applications, fast and easy analysis, and environmentally friendly. The technical solution is: it is characterized in that it includes the following steps: (1) transporting the tested water sample solution through a pump; (2) the water sample solution flows through the filter column; (3) the water sample flowing through the filter column enters the fluorescence pool; ( 4) Use a high-frequency pulse excitation light source-deep ultraviolet laser diode to pump a solid-state laser to generate a continuous-band light source, irradiate the fluorescent cell, use the spectral spectroscopic device-grating on the detection side of the fluorescent cell to perform spatial light splitting, and form λ1- after dispersion The spectral band of λ2; (5) The CCD photodetector located at the detection window collects the spectral data of λ1-λ2 at the same time, and obtains the wavelength-light intensity two-dimensional spectrum through internal transformation and time series integration; (6) The data processing system passes through the ocean Computational Analysis of Marine Bacterial Abundance and Diversity by Bacterial Fluorescence Recognition Patterns.

Description

technical field [0001] The invention belongs to the field of detection methods for in-situ abundance and diversity of marine bacteria, in particular to a detection method for in-situ abundance and diversity of marine bacteria based on laser-induced fluorescence, which has a wide range of applications, quick and easy analysis, and is environmentally friendly. This method uses the optical characteristics of the fluorescence spectrum produced by the laser-induced fluorescence process and the "characteristic spectrum" of various marine bacteria itself - that is, the fluorescence spectra produced by various marine bacteria in the laser-induced fluorescence process are compared in different bands, Combined with marine bacterial fluorescence recognition patterns, the abundance and diversity of marine bacteria can be analyzed. Background technique [0002] Marine bacteria are an important part of the marine ecosystem. The study of bacteria in the marine environment is not only one ...

AI Technical Summary

Problems solved by technology

Due to the need to collect and prepare samples for these technologies, strict requirements for sample injection, and the need to make fluorescent markers, it is difficult to apply to the detection of bacteria in the marine field

At the same time, fluorescence microscope technology and plate culture methods have proved that in the ocean environment, only a small amount of marine bacteria can form colonies through traditional fixed plate technology, and some bacteria are in a living non-culturable state, which cannot be fully reflected by conventional isolation and culture methods. The state of marine bacterial resources and ecological functions, it is difficult to fully understand the abundance and diversity of bacteria and their ecological significance and role in the marine carbon cycle

[0004] In addition, the method adopts the mode of on-site sampling and analysis in the laboratory, that is, the method of on-site and real-time measurement cannot be realized, and other interfering substances are easily introduced during the sample transportation and processing process, which affects the accuracy of the analysis.

The detection of marine bacterial abundance and diversity involves certain environmental conditions and complex processes, so it is impossible to guarantee that there will be no possibility of secondary pollution in this process. The incompatibility of the environment, coupled with the large difference between the laboratory environment and the marine environment, leads to a large difference between the measurement results and the actual situation, and the accuracy and reliability of the results are questioned

[0005] The above methods have the following defects in varying degrees: 1. It must be completed in the laboratory, and the application cannot be real-time on site, and the scope is limited

2. The analysis takes a long time, at least a few days

3. The analysis process is complicated, the conditions are harsh, the energy consumption is high, and the technical level of the experimenters is high.

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