In situ detection of abundance and diversity of marine bacteria based on laser-induced fluorescence

A laser-induced fluorescence, marine bacteria technology, applied in fluorescence/phosphorescence, material excitation analysis, etc., can solve the problems of harsh conditions, long analysis duration, complicated analysis process, etc., achieve long-term reliable work, wide application range, fast analysis easy effect
CN104089937BActive Publication Date: 2017-01-04OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
Publication Date
2017-01-04

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Abstract

A laser-induced fluorescence-based in situ detection method for the abundance and diversity of marine bacteria with a wide range of applications, fast and easy analysis, and environmentally friendly. The technical solution is: it is characterized in that it includes the following steps: (1) transporting the tested water sample solution through a pump; (2) the water sample solution flows through the filter column; (3) the water sample flowing through the filter column enters the fluorescence pool; ( 4) Use a high-frequency pulse excitation light source-deep ultraviolet laser diode to pump a solid-state laser to generate a continuous-band light source, irradiate the fluorescent cell, use the spectral spectroscopic device-grating on the detection side of the fluorescent cell to perform spatial light splitting, and form λ1- after dispersion The spectral band of λ2; (5) The CCD photodetector located at the detection window collects the spectral data of λ1-λ2 at the same time, and obtains the wavelength-light intensity two-dimensional spectrum through internal transformation and time series integration; (6) The data processing system passes through the ocean Computational Analysis of Marine Bacterial Abundance and Diversity by Bacterial Fluorescence Recognition Patterns.
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Description

technical field

[0001] The invention belongs to the field of detection methods for in-situ abundance and diversity of marine bacteria, in particular to a detection method for in-situ abundance and diversity of marine bacteria based on laser-induced fluorescence, which has a wide range of applications, quick and easy analysis, and is environmentally friendly. This method uses the optical characteristics of the fluorescence spectrum produced by the laser-induced fluorescence process and the "characteristic spectrum" of various marine bacteria itself - that is, the fluorescence spectra produced by various marine bacteria in the laser-induced fluorescence process are compared in different bands, Combined with marine bacterial fluorescence recognition patterns, the abundance and diversity of marine bacteria can be analyzed. Background technique

[0002] Marine bacteria are an important part of the marine ecosystem. The study of bacteria in the marine environment is not only one ...

Claims

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