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Preparation method of artificial ligament modified by calcium strontium/gelatin biomimetic coating

An artificial ligament and gelatin technology, applied in the field of biomedicine, can solve the problems of poor repeatability of experimental results, low osteogenic differentiation rate, uneven coating, etc., to improve biocompatibility and increase the probability of tendon-bone healing , the effect of facilitating cell adhesion

Active Publication Date: 2017-07-07
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research results also show that the artificial direct coating method has problems such as uneven coating, lack of stability, poor reproducibility of experimental results, and low osteogenic differentiation rate.

Method used

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  • Preparation method of artificial ligament modified by calcium strontium/gelatin biomimetic coating
  • Preparation method of artificial ligament modified by calcium strontium/gelatin biomimetic coating
  • Preparation method of artificial ligament modified by calcium strontium/gelatin biomimetic coating

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Under background vacuum 5 x 10 -2 Pa, under the condition of power 200W, use H 2 Plasma treated the PET artificial ligament for 8 minutes, then soaked in 10mM acrylic acid monomer grafting solution for 2 hours, and finally washed with pure water and dried in vacuum for later use;

[0038] Add 2 g of bone gelatin particles to 100 ml of deionized water to swell, and then heat to 55 ° C to form a 2wt.% bone gelatin solution; then add 6 ml of 40 mM CaCO 3 and 6ml 20mM SrNO 3 Add it to the gelatin solution, adjust the pH value to 7, and stir at 25°C for 10 minutes to obtain a uniformly mixed solution; soak the pretreated artificial ligament in this mixed solution to fully combine the free Ca and Sr ions and gelatin with the ligament , take the ligament out of the filter paper to dry, and finally soak in 500ml 60mM freshly prepared Na 2HPO 4 The solution was irradiated by sunlight for 20 minutes, and the above steps were repeated 20 times to obtain a new type of artificia...

Embodiment 2

[0044] 8 x 10 in background vacuum -2 Pa, under the condition of 400W power, use Ar plasma to treat the PET artificial ligament for 20 minutes, then put it into 100mM polyethylene glycol monomer grafting solution and soak for 3 hours, and finally wash it with pure water and dry it in vacuum for later use;

[0045] Add 10 g of bone gelatin particles to 100 ml of deionized water to swell, then heat to 50 ° C to form a 10% bone gelatin solution, and then add 6 ml of 120 mM CaCO 3 Add 6ml of 30mM strontium dichloride to the gelatin solution, adjust the pH value to 8, and stir for 20 minutes at 25 degrees Celsius to obtain a uniformly mixed solution; soak the pretreated artificial ligament in the mixed solution, and stir for 45 minutes to react. Make the free Ca and Sr ions and gelatin fully combined with the ligament, take out the filter paper to dry the ligament, and finally soak it in 600ml 90mM freshly prepared sodium phosphate solution, expose it to simulated sunlight for 30mi...

Embodiment 3

[0047] Add 10g of bone gelatin particles to 200ml of deionized water to swell, then heat to 60°C to form a 5% bone gelatin solution, add 6ml of 300mM calcium nitrate and calcium carbonate mixture, and 6ml of 50mM strontium nitrate and strontium chloride mixture In 5% gelatin solution, adjust the pH value to 7.4, stir at 25°C for 30 minutes to obtain a well-mixed solution; then vacuum 7×10 -2 Pa, under the condition of power 350W, use H 2 Plasma treated the PET artificial ligament for 15 minutes, then soaked it in 55mM heparin monomer grafting solution for 6 hours, and finally washed it with pure water and dried it in vacuum; soaked the pretreated artificial ligament in the mixed solution, stirred and reacted for 50 minutes, so that the free Ca and Sr ions and gelatin are fully combined with the ligament, the ligament is taken out of the filter paper to dry, and finally soaked in 250ml 75mM freshly prepared mixed solution of potassium dihydrogen phosphate and potassium phosphat...

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Abstract

The invention discloses a preparation method of artificial ligament modified by calcium strontium / gelatin bionic coating; adding strontium salt and calcium salt into gelatin solution, stirring to fully dissolve, and obtaining a uniformly mixed solution; plasma treatment surface modified artificial ligament Immerse the ligament in the solution, add phosphate, stir to form a mixed solution, and then expose it to sunlight or simulated sunlight for 20 to 60 minutes, and dry it with filter paper; repeat the third step until the preset number of mineralization cycles is reached, That's it. The method of the present invention simulates the biomineralization mechanism of natural bone, uses natural polymer or gelatin as a substrate or template to deposit an apatite inorganic salt layer, and performs composite coating on the surface of the material, so that the artificial ligament is loaded with nanoscale strontium hydroxyapatite Stone, thereby improving the biocompatibility and osteoconductivity of the material, so that it can meet the requirements of clinical medicine in terms of biological activity and mechanical properties.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a preparation method of calcium strontium / gelatin bionic coating modified artificial ligament. Background technique [0002] Polyethylene terephthalate (PET) artificial ligaments have been widely used clinically as grafts for the reconstruction of cruciate ligament injuries of the knee joint, and the short-term curative effect and safety are relatively satisfactory, but there are still poor tendon-bone healing And concurrent synovitis and other problems. Therefore, it is particularly important to improve the biocompatibility of PET materials and promote the tendon-bone healing of the artificial ligament. [0003] Surface coating treatment of PET artificial ligament grafts to improve the hydrophilicity, biocompatibility and osteoconductivity of biomaterials has become the focus of the development of orthopedic biomaterials. Reasonable use of multiple components to compound biomedical mat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08J7/04C09D189/00C09D7/12C08J7/12C08L67/02A61L27/34A61L27/30A61L27/18
Inventor 张鹏陈世益陈俊蒋佳陈天午万方
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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