Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Dehydrogenase electrode and preparation method thereof

A dehydrogenase and electrode technology, which is applied in the field of biosensors to achieve the effects of reducing activity loss, realizing process optimization control, and improving the hydrophilicity of electrode surface

Active Publication Date: 2014-11-05
BIOLOGY INST OF SHANDONG ACAD OF SCI
View PDF6 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Although the dehydrogenase electrode has broad application prospects and market value, there are still many key problems to be solved in the research of this type of electrode.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Dehydrogenase electrode and preparation method thereof
  • Dehydrogenase electrode and preparation method thereof
  • Dehydrogenase electrode and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Take the reduced coenzyme Ⅰ (NADH) in the test buffer solution of this electrode as an example (for the principle of electrode response, see figure 1 ). Its measurement steps are as follows:

[0031] a. The modified electrode prepared by drop-coating the carbon nanotube / protamine composite material on the surface of the base electrode was used as the working electrode, the Ag / AgCl (3M KCl) electrode was used as the reference electrode, and the foil electrode was used as the counter electrode. (CHI 760D) electrochemical workstation for determination. The modified electrode, Ag / AgCl (3M KCl), foil electrode and CHI 760D electrochemical workstation are connected. Insert the working electrode directly into the measuring cell filled with buffer solution, and record the initial cyclic voltammetry curve.

[0032] Electrode preparation process: Take 20mg of protamine, add it into 5mL ultrapure water, prepare 4mg / mL protamine aqueous solution, and place it for later use. 5 m...

Embodiment 2

[0036] First, the buffer solution was taken to configure two spiked samples, the concentrations were 10 -6 and 10 -7 , measure oxidation peak current according to embodiment 1, according to peak current value, contrast standard working curve (such as image 3 ) to calculate the corresponding concentration.

Embodiment 3

[0038] Test the content of ethanol in the buffer solution with a dehydrogenase electrode (for the principle of electrode response, see Figure 4 ). Its measurement steps are as follows:

[0039] a. The alcohol dehydrogenase electrode was used as the working electrode, the Ag / AgCl (3M KCl) electrode was used as the reference electrode, and the foil electrode was used as the counter electrode, and Shanghai Chenhua (CHI 760D) electrochemical workstation was used for determination. The enzyme electrode, Ag / AgCl (3M KCl), foil electrode and CHI 760D electrochemical workstation are connected. Insert the dehydrogenase electrode directly into the measuring cell filled with buffer solution, record the initial cyclic voltammetry curve, add ethanol of known concentration into the measuring cell, and an oxidation peak appears on the cyclic voltammetric curve (such as Figure 5 ), draw a standard curve according to the oxidation peak current size and ethanol concentration after adding et...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a dehydrogenase electrode and a preparation method thereof. The dehydrogenase electrode comprises a base electrode, an electron conduction layer coating the base electrode and a dehydrogenase layer coating the electron conduction layer. The electron conduction layer comprises a bionic nanocomposite material. The dehydrogenase layer comprises a bionic nanocomposite material / dehydrogenase compound. The preparation method comprises the following steps of a, coating the base electrode with an ultrasonically dispersed suspending liquid of a carbon nano-material and a biomacromolecule so that the electron conduction layer is obtained, and b, adding dehydrogenase into the suspending liquid, carrying out ultrasonic treatment to obtain a cured dehydrogenase system, and dropwisely coating the electron conduction layer with the cured dehydrogenase system so that the dehydrogenase layer is obtained. The dehydrogenase electrode can realize coenzyme electrochemical regeneration at low potential and has the advantages of operation simpleness, high detection sensitivity, good electrode stability and reappearance and small enzyme activity loss.

Description

technical field [0001] The invention relates to the field of biosensors, in particular to a dehydrogenase electrode and a preparation method thereof. Background technique [0002] Since the appearance of the enzyme electrode in the 1960s, its technical research and application have achieved tremendous development, but almost all enzymes used in analytical components are oxidases. Dehydrogenase is also a type of oxidoreductase, which is different from oxidase in that the catalytic function of the enzyme needs to rely on a coenzyme that is easy to gain and lose electrons. Since the coenzyme is used as the transfer body of electrons and hydrogen, the catalytic reaction of the dehydrogenase is not affected by the oxygen in the system, which is particularly prominent in the practical application of the sensor. At the same time, compared with oxidases, dehydrogenases have a wide variety of sources and generally have higher selectivity to substrates. Therefore, dehydrogenase elect...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N27/327
Inventor 陈燕
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com