Single-wall carbon nanohorn-platinum palladium nanoparticles and cytomegalovirus pp65 antibody modified electrode and its preparation method and application

Abstract

The invention discloses an electrode modified with single-walled carbon nanohorn-platinum and palladium nano particles and cytomegalovirus pp65 antibody, and a preparation method and an application thereof. The electrode is prepared by the steps of treating single-walled carbon nanohorn dispersed by a perfluorinated sulfonic acid-polytetrafluoroethylene copolymer membrane, then absorbing thionine, and then combining with the single-walled carbon nanohorn-platinum and palladium nano particles, incubating the cytomegalovirus pp65 antibody and finally sealing with horse radish peroxidase (HRP). The prepared electrode is combined with single-walled carbon nanohorn modified with chitosan; since the single-walled carbon nanohorn has large specific surface area and good biocompatiability, after being modified with amino, the single-walled carbon nanohorn is further beneficial to stabilization of platinum and palladium particles, improvement of the combined quantity of the cytomegalovirus pp65 antibody and increase of the peak current value; in addition, the platinum and palladium particles and HRP can catalyze hydrogen peroxide and amplify a detection signal, thus improving the sensitivity of detection on cytomegalovirus antigen with low concentration.

Description

technical field [0001] The invention belongs to the field of diagnosis, and specifically relates to a single-walled carbon nanohorn-platinum palladium nanoparticle and a cytomegalovirus pp65 antibody modified electrode, and also relates to a preparation method and application of the electrode. Background technique [0002] Cytomegalovirus (CMV) is a family of herpes viruses with strict species specificity. In developed countries, the invisible infection rate of CMV in adults is 40% to 90%, and in my country, the invisible infection rate of CMV is as high as about 90%. CMV is the most common pathogen causing congenital infection of the fetus. Epidemiological survey results show that about 0.5% to 2.5% of newborns have been infected with CMV. Pregnant women infected with CMV can transmit vertically to the fetus or newborn through the placenta, birth canal, and breast-feeding, and can also transmit horizontally through close contact, eventually leading to fetal malformation, ...

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Problems solved by technology

[0003] Existing detection methods mainly include cell culture, virological detection, immunological detection, nucleic acid detection, etc., among which cell culture is the gold standard for diagnosing CMV infection, but these methods have the disadvantages of cumbersome operation, low detection efficiency, and poor specificity.

For example, cell culture is expensive, requires high technical personnel, and clinical application is limited; virological examination needs to isolate and cultivate CMV from the blood, urine, saliva or tissue of the subject, and the operation process is time-consuming and laborious; serological CMV The detection of IgG and IgM antibodies has poor specificity, may have false negatives for newborns or those with severe immunodeficiency, and will be interfered by rheumatoid factors; molecular biology nucleic acid detection requires professionals and special equipment, which is expensive and impossible distinguish latent infection

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