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Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves

A technology of surface-enhanced Raman and pesticide residues, which is applied in the rapid screening of pesticide residues in tea, and in the field of rapid screening of pesticide residues in tea with surface-enhanced Raman spectroscopy, can solve the problem that the weak information of pesticides is difficult to analyze and the detection limit needs to be further improved Improvement, method accuracy, poor stability and other problems, to achieve the effect of simple sample pretreatment, high accuracy and precision, and improved accuracy

Inactive Publication Date: 2014-11-12
JIANGXI AGRICULTURAL UNIVERSITY
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although surface-enhanced Raman spectroscopy can enhance the Raman intensity and at the same time quench the fluorescence signal, the weak information of pesticides in tea will be completely covered by the signal of high-concentration components in tea itself, making it difficult to analyze the weak information of pesticides
[0007] The method disclosed above can achieve the purpose of enhancing the signal of pesticide molecules in agricultural products by using surface-enhanced Raman spectroscopy, but it cannot fully remove the influence of matrix substances. The detection limit of the method needs to be further improved, and the accuracy and stability of the method are poor. , to apply these methods to the rapid detection of pesticide residues in tea, the detection method needs to be further improved

Method used

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  • Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves
  • Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves
  • Method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves

Examples

Experimental program
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Effect test

Embodiment 1

[0043] Example 1: Rapid detection of thiabendazole pesticide residues in fresh tea leaves by surface-enhanced Raman spectroscopy

[0044] (1) Preparation of silver nano-reinforced substrate: Pour a certain concentration of silver nitrate solution (36mg silver nitrate dissolved in 200mL ultrapure water) into a flask, place it on a constant temperature magnetic stirrer, heat it to boiling rapidly at high temperature, and within 2 minutes Gradually drop a 1% trisodium citrate solution (60mg trisodium citrate dissolved in 6mL ultrapure water) while stirring at a speed of 200r / min. At this time, the solution slowly changes from transparent to light brown. After 25 minutes of reaction, A gray-green liquid was obtained. After cooling down at room temperature, pour the above appropriate amount of silver colloid solution into a centrifuge tube, pour off a small amount of supernatant after centrifugation, then add an appropriate amount of ultrapure water to the centrifuge tube, and mix ...

Embodiment 2

[0054] Example 2: Rapid detection of thiabendazole pesticide residues in dry tea by surface-enhanced Raman spectroscopy

[0055] (1) Preparation of silver nano-reinforced substrate: Pour a certain concentration of silver nitrate solution (36mg silver nitrate dissolved in 200mL ultrapure water) into a flask, place it on a constant temperature magnetic stirrer, heat it to boiling rapidly at high temperature, and within 2 minutes Gradually drop a 1% trisodium citrate solution (60mg trisodium citrate dissolved in 6mL ultrapure water) while stirring at a speed of 200r / min. At this time, the solution slowly changes from transparent to light brown. After 25 minutes of reaction, A gray-green liquid was obtained. After cooling down at room temperature, pour the above appropriate amount of silver colloid solution into a centrifuge tube, pour off a small amount of supernatant after centrifugation, then add an appropriate amount of ultrapure water to the centrifuge tube, and mix well with...

Embodiment 3

[0065] Example 3: Rapid detection of chlorpyrifos pesticide residues in fresh tea leaves by surface-enhanced Raman spectroscopy

[0066] (1) Preparation of silver nano-reinforced substrate: Pour a certain concentration of silver nitrate solution (36mg silver nitrate dissolved in 200mL ultrapure water) into a flask, place it on a constant temperature magnetic stirrer, heat it to boiling rapidly at high temperature, and within 2 minutes Gradually drop a 1% trisodium citrate solution (60mg trisodium citrate dissolved in 6mL ultrapure water) while stirring at a speed of 200r / min. At this time, the solution slowly changes from transparent to light brown. After 25 minutes of reaction, A gray-green liquid was obtained. After cooling down at room temperature, pour the above appropriate amount of silver colloid solution into a centrifuge tube, pour off a small amount of supernatant after centrifugation, then add an appropriate amount of ultrapure water to the centrifuge tube, and mix w...

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Abstract

The invention provides a method for fast detecting surface enhancing Raman spectrums of pesticide residues in tea leaves and belongs to the technical field of fast tea leaf pesticide detection. According to the method, acetonitrile is adopted for extracting active ingredients in the tea leaves, extract liquor comprises the ingredients of the tea leaves and the pesticide ingredients contained in the tea leaves, ferroferric oxide nanometer particles and graphitized carbon are used for removing fluorescent materials such as chlorophyll and L-theanine in the extract liquor so as to improve the accuracy for detecting the surface enhancing Raman spectrums of the pesticide residues in the tea leaves, and the purpose of fast detecting the pesticide residues in the tea leaves can be achieved. According to the method for fast detecting the surface enhancing Raman spectrums of the pesticide residues in the tea leaves, sample pretreatment is simple and fast, detecting a single sample only needs about 15 minutes, the pesticide residues in tea leaf samples can be analyzed fast and accurately in a quantitative mode, and the method can be used for fast detecting the samples on site in a large-scale mode.

Description

technical field [0001] The invention relates to the field of rapid screening methods for pesticide residues in tea, in particular to a surface-enhanced Raman spectrum rapid screening method for pesticide residues in tea, and belongs to the technical field of rapid detection of pesticides in tea. Background technique [0002] Tea leaves are rich in medicinal ingredients such as tea polyphenols, amino acids, and caffeine, which have various effects such as lowering blood fat, anti-cancer, preventing cardiovascular diseases, delaying aging, and losing weight. However, my country's tea production system is dominated by individual tea farmers, and there are certain difficulties in the promotion of rational use of pesticides and residue control. In addition, there have been problems such as lax pesticide supervision and insufficient supervision of tea quality and safety for a long time. Pesticide residues in tea Exceeding the standard is commonplace. [0003] The detection of pest...

Claims

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Application Information

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IPC IPC(8): G01N21/65
Inventor 吴瑞梅刘木华艾施荣蔺磊王晓彬吴彦红范苑严霖元
Owner JIANGXI AGRICULTURAL UNIVERSITY
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