Specific polypeptide modified colorimetric sensor and making method thereof

A technology of colorimetric sensor and polypeptide modification, which is applied in the direction of color/spectral characteristic measurement, etc., can solve the problems that have not been reported, and achieve the effect of wide detection range, wide application prospect and high sensitivity

Inactive Publication Date: 2014-11-26
SHANGHAI UNIV
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Problems solved by technology

Specific peptides can have specific effects on their corresponding substances (such as ions, small molecules, proteins, nucleotides, etc.) Other materials are favored by researchers, making them have great application prospects in the field of biosensors. At present, researchers mainly use their characteristics to design and construct colorimetric detection systems for enzyme activity detection and specific protein detection, but There is no report on the detection of heavy metal ions, especially copper ions

Method used

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  • Specific polypeptide modified colorimetric sensor and making method thereof

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Embodiment 1

[0020] In this embodiment, the UV spectrometry method and steps of the interaction between specific polypeptide-modified gold nanoparticles and copper ions are as follows:

[0021] (1) Preparation of gold nanoparticles specifically modifying the polypeptide: first, gold nanoparticles with a particle diameter of 13 nm were prepared. Add a chloroauric acid solution with a mass concentration of 0.01% into a round-bottomed flask equipped with a reflux condensing device, heat and stir rapidly, and when the chloroauric acid solution boils for 2-3 minutes, quickly add 3.5 mL at one time with a mass concentration of 1% trisodium citrate solution, continue to heat for 15 minutes, when the color of the solution gradually turns into wine red, stop heating and continue to stir for 30 minutes, let it cool to room temperature, and store it at 4°C for later use. Then, 20 μL of the specific peptide solution with a concentration of 1 mg / mL was added to 980 μL of HEPES solution and mixed evenly...

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Abstract

The invention relates to a specific peptide modified colorimetric sensor and a making method thereof. The colorimetric sensor is used for detecting copper ions. The sensor uses a gold-mercapto key to make the surface of gold nanoparticles to be modified by a specific peptide, the addition of copper ions to be measured changes the conformation of the specific polypeptide from alpha-helix to beta-folding, and the gold nanoparticles mutually aggregate by using beta-folding fragments formed by the surface modification specific polypeptide in order to change the absorbance of a solution and generate color difference. The copper ions are detected by the gold nanoparticles modified by the specific peptide for the first time, and the colorimetric sensor of the gold nanoparticles modified by the specific polypeptide has very high sensitivity, simplicity and specificity on the copper ions, has a wide detection range, has potential application values in the detection of the content of the copper ions in drinking water, serum, rivers, sewage and other solutions, and also has the advantages of convenient operation, high sensitivity and wide application prospect.

Description

technical field [0001] The invention relates to a colorimetric sensor and a preparation method thereof, in particular to a specific polypeptide-modified colorimetric sensor and a preparation method thereof. Background technique [0002] Copper is an essential trace heavy metal element in animals and plants. In the body, an appropriate amount of copper plays an important role in physiological processes such as energy metabolism, oxygen transport and signal transduction. However, excessive levels of copper in the body can be life-threatening. Excessive copper in the body may lead to a series of neurodegenerative diseases, such as: Menkers syndrome, Wilson's syndrome and Alzheimer's disease schizophrenia etc. The U.S. Environmental Protection Agency has stipulated that the safe limit of copper content in drinking water is 1.33ppm (about 20μM). The copper ion content is not higher than 1.0mg / L (about 15.625μM). It can be seen that how to quickly and efficiently detect copper...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
Inventor 李根喜陈红霞张江江祁方杰
Owner SHANGHAI UNIV
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