A method for rapidly obtaining menuploid plants of Violet japonica

A technology of purple cauliflower and purple flower, which is applied in the field of plant tissue culture, can solve the problems such as no report on the application of the microspore culture technology of purple cauliflower, and achieves the effects of operability, workload reduction and quality improvement.

Active Publication Date: 2016-07-06
天津天隆在田农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although scholars at home and abroad have done a lot of in-depth research on the application of microspore culture technology in cruciferous plants, there is no report on the application of microspore culture technology in purple lily.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The cultivation method is carried out as follows.

[0028] (1) Culture medium preparation: comprise the culture medium of microspore different culture stages, its component and the weight that each component contains in every liter of medium are:

[0029] 1) B5 washing medium: B5 liquid medium 1L + sucrose 30g / L, pH6.0, high temperature and high pressure;

[0030] The B5 liquid medium, in 1L, consists of: NaH 2 PO 4 2H 2 O169.5mg, KNO 3 2500mg, (NH 4 ) 2 SO 4 134mg, MgSO 4 ·7H 2 O500mg, MnSO 4 4H 2 O10mg, H 3 BO 3 3mg, ZnSO 47H2O2mg, KI0.75mg, Na 2 MoO 4 2H 2 O0.25mg, CuSO 4 ·5H 2 O0.025mg, CoCl 2 ·6H 2 O0.025mg, Na 2 ‐EDTA37.3mg, FeSO 4 ·7H 2 O27.8mg, CaCl 2 .2H 2 O150mg, VB110mg, VB61mg, VPP1mg, inositol 100mg and the rest of distilled water.

[0031] 2) Embryoid body differentiation medium: B5 medium + sucrose 20g / L, agar 10g / L, pH6.0, high temperature and high pressure sterilization;

[0032] 3) NLN-13 induction medium, NLN liquid medium 1...

Embodiment 2

[0046] In addition to step (1) 1) B5 washing medium: B5 liquid medium 1L + sucrose 30g / L, pH6.0, high temperature and high pressure; 2) NLN‐13 induction medium: NLN‐13 liquid medium 1L + sucrose 130g / L , pH6.1, filter sterilized; 3) embryoid body differentiation medium: B5 medium + sucrose 20g / L, agar 11g / L, pH6.0, high temperature and high pressure sterilization; 4) rooting medium: MS medium + Sugar 20g / L, agar 7g / L, pH5.9, high temperature and high humidity sterilization;

[0047] (2) The culture method that co-cultivation of purple lily microspores and cabbage anthers promotes embryogenesis:

[0048] 1) Selection of flower buds: Take the flower buds with a petal-to-anther length ratio of 1.0 for purple cauliflower, 1.0 for cabbage, late mononucleate to early binucleate, healthy, and free from diseases and insect pests, as donors for microspore culture;

[0049] 2) Sterilization of flower buds: use 1g of mercuric liter + 1L of sterile water to prepare a sterilizing solution...

Embodiment 3

[0057]In addition to step (1) 1) B5 washing medium: B5 liquid medium 1L + sucrose 30g / L, pH6.0, high temperature and high pressure; 2) NLN‐13 induction medium: NLN‐13 liquid medium 1L + sucrose 130g / L , pH6.1, filter sterilized; 3) embryoid body differentiation medium: B5 medium + sucrose 20g / L, agar 11g / L, pH6.0, high temperature and high pressure sterilization; 4) rooting medium: MS medium + Sugar 20g / L, agar 7g / L, pH5.9, high temperature and high humidity sterilization;

[0058] (2) The culture method that co-cultivation of purple lily microspores and cabbage anthers promotes embryogenesis:

[0059] 1) Selection of flower buds: take the flower buds with a petal-to-anther length ratio of 1.1 for purple cauliflower, 1.1 for cabbage, late mononucleate to early binucleate, healthy, and free from diseases and insect pests, as donors for microspore culture;

[0060] 2) Sterilization of flower buds: use 1g of mercuric liter + 1L of sterile water to prepare a sterilizing solution;...

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PUM

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Abstract

The invention discloses a method for rapidly obtaining ploidy plants of Viola japonica, comprising: taking purple cauliflower flower buds, adding B5 washing medium after sterilization to make a suspension, filtering the obtained filtrate to obtain precipitates through centrifugation; adding NLN-13 in sequence The mixture of induction medium and activated carbon was obtained to obtain microspore suspension; it was divided into sterile petri dishes, and sterile cabbage anthers were added, and then heat-shocked after Parafilm sealing; after conventional culture, cotyledon-type embryoid bodies were obtained; inoculated into The embryoid body is kept in the differentiation medium until it is differentiated, and sprouts are regenerated; the regenerated shoots are cut and inoculated on the rooting medium for rooting culture, and the regenerated plants are obtained through hardening and transplanting. In the method, the cabbage anthers which are easy to germinate and the free microspores of purple cauliflower which are difficult to germinate are mixed and cultivated in a certain proportion, thereby promoting the development of the embryos of the violet cauliflower which is difficult to germinate, obtaining a large number of regenerated plants, and improving the culture efficiency.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for cultivating naploid plants of Violet flower. Background technique [0002] Purple cauliflower (Brassica oleracea var. botrytis) belongs to Brassica Brassica Brassica species of Brassicaceae. It is native to Western Europe and Italy. It is a variety of cauliflower. Other traits are similar to cauliflower. Purple cauliflower is rich in nutrition, rich in protein, vitamin C, and less in crude fiber. In addition, it also contains vitamins A, B1, B2, sucrose, fructose, selenium, etc. It is delicious in flavor, can improve human immune function, promote liver detoxification, and enhance human immunity. Physical fitness and disease resistance. The selenium contained in it can inhibit the growth of cancer cells. [0003] At present, most of the varieties of purple cauliflower used in production are imported from foreign countries, and the seeds are expensive,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C05G1/00
Inventor 张振超毛忠良潘跃平吴国平王建华戴忠良秦文斌姚悦梅潘永飞肖燕孙春青马志虎孙国胜
Owner 天津天隆在田农业科技有限公司
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