Cross-linked hyaluronic acid gel microparticle and preparation method thereof
A technology of cross-linking hyaluronic acid and hyaluronic acid, which is applied in the field of medical plastic surgery, can solve the problems that the dense structure is not conducive to the removal of residual cross-linking agents, affects the degradation time, and affects the operator's comfort, so as to reduce cross-linking The effect of reducing agent residue, increasing specific surface area, and ensuring safety
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Embodiment 1
[0051] Step 1, preparation of cross-linked hyaluronic acid gel:
[0052] 1. Weigh 1g of hyaluronic acid raw material;
[0053] 2. Add 1,4-butanediol diglycidyl ether, a cross-linking agent with 3% mass of hyaluronic acid raw material, to 15 ml of sodium hydroxide solution with a pH value of 11, and mix well to obtain solution A;
[0054] 3. Then dissolve the hyaluronic acid raw material in solution A, and after homogeneous stirring, obtain a hyaluronic acid sodium hydroxide gel with a concentration of 67 mg / ml;
[0055] 4. Then place it in a water bath at 65°C for a cross-linking reaction for 1.5 hours to obtain a cross-linked hyaluronic acid gel. Step 2, preparation of dehydration cross-linked hyaluronic acid film:
[0056] 1. Put the cross-linked hyaluronic acid gel obtained in step 1 into a roller press, and extrude it into a cross-linked hyaluronic acid gel film with a thickness of 0.1 mm.
[0057] 2. Dialyze the cross-linked hyaluronic acid gel film in 0.1g / L disodium ...
Embodiment 2
[0069] Step 1, preparation of cross-linked hyaluronic acid gel:
[0070] 1. Weigh 1g of hyaluronic acid raw material;
[0071] 2. Add ethylene glycol diglycidyl ether, a cross-linking agent with a mass of 1% hyaluronic acid raw material, to 10 ml of potassium hydroxide solution with a pH value of 13, and mix well to obtain solution A;
[0072] 3. Then dissolve the hyaluronic acid raw material in solution A, and after homogeneous stirring, obtain a hyaluronic acid potassium hydroxide gel with a concentration of 100 mg / ml;
[0073] 4. Then place it in a water bath at 45°C for a cross-linking reaction for 3 hours to obtain a cross-linked hyaluronic acid gel.
[0074] Step 2, preparation of dehydration cross-linked hyaluronic acid film:
[0075] 1. Put the cross-linked hyaluronic acid gel obtained in step 1 into a roller press, and extrude it into a cross-linked hyaluronic acid gel film with a thickness of 0.3 mm.
[0076] 2. Dialyze the cross-linked hyaluronic acid gel film in...
Embodiment 3
[0087] Step 1, preparation of cross-linked hyaluronic acid gel:
[0088] 1. Weigh 1g of hyaluronic acid raw material;
[0089] 2. Add glycerol triglycidyl ether, a cross-linking agent with a mass of 5% hyaluronic acid raw material, to 12 ml of potassium hydroxide solution with a pH value of 9, and mix well to obtain solution A;
[0090] 3. Then dissolve the hyaluronic acid raw material in solution A, and after homogeneous stirring, obtain a hyaluronic acid potassium hydroxide gel with a concentration of 83 mg / ml;
[0091] 4. Then place it in a water bath at 50°C for 2.5 hours of cross-linking reaction to obtain a cross-linked hyaluronic acid gel. Step 2, preparation of dehydration cross-linked hyaluronic acid film:
[0092] 1. Put the cross-linked hyaluronic acid gel obtained in step 1 into a roller press, and extrude it into a cross-linked hyaluronic acid gel film with a thickness of 0.2mm;
[0093] 2. Place the cross-linked hyaluronic acid gel film in 0.3 g / L disodium hyd...
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