Listeriosis chromogenic medium

A technology of chromogenic culture medium and Listeria, applied in the field of Listeria chromogenic culture medium, can solve problems such as inability to infer and calculate, and achieve the effects of low cost, easy observation, and favorable promotion and application

Inactive Publication Date: 2014-12-10
QINGDAO RUNXIN WEIYE TECH & TRADE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, it is still not possible to infer and calculate the medium formula suitable for a certain strain from the basic principles of biochemical reactions. We can only use the basic theories of biochemistry, cell biology, microbiology, etc., and refer to the more suitable ones used by predecessors. The empirical formula of a certain type of strain, combined with the characteristics of the strains used and the product, uses small fermentation equipment such as shake flasks and glass tanks, and selects a more suitable medium according to certain experimental designs and experimental methods

Method used

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Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] A Listeria chromogenic medium, the components of the medium include sugar fermentation base broth, 1.0g / L disodium hydrogen phosphate, 10.0g / L glycine, 0.5g / L lithium chloride, 2.5g / L phenylethyl alcohol, 15.0g / L agar, 0.1g chromogenic agent, pH value is 7.2-7.4; Described sugar fermentation basic broth contains tryptone 10.0g, beef extract powder 3.0 in every liter of sugar fermentation basic broth g, sodium chloride 5.0g, bromocresol purple 0.04g, and the rest are distilled water.

[0022] Weigh 14.2g of basic medium and add 180ml of distilled water, heat to dissolve and keep stirring; take another 1.2g of Listeria additive, add 20ml of sterile water, and add some glass balls, keep vibrating to make it emulsify evenly. Both were autoclaved at 121°C for 15 minutes. When it is cooled to about 50°C, add the Listeria additive to the basal medium, and add 1 each of Listeria antibacterial agent 1 and 2, mix well, and pour into a sterile plate. Make the thickness of the c...

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Abstract

The invention belongs to the field of microorganisms and in particular relates to a listeriosis chromogenic medium. The medium comprises the following components: bromcersol purple broth base, 1.0g / L of sodium hydrogen phosphate, 10.0g / L of glycine, 0.5g / L of lithium chloride, 2.5g / L of phenethyl alcohol, 15.0g / L of agar and 0.1g / L of chromogenic agent, wherein the pH value of the medium is 7.2-7.4; every 1L of bromcersol purple broth base contains 10.0g of tryptone, 3.0g of beef extract powder, 5.0g of sodium chloride, 0.04g of bromcresol purple and the balance of distilled water. The listeriosis chromogenic medium has the beneficial effects that the medium is simple in components, convenient in preparation operation and low in cost and is beneficial to popularization and application; the bacteria grow rapidly and the mutation rate is low; the medium is convenient to observe and analyze.

Description

technical field [0001] The invention belongs to the field of microorganisms, in particular to a chromogenic culture medium for Listeria. Background technique [0002] The diversity and variability among microorganisms in the world and the ecological complexity of species habitats have brought about the breadth, complexity and permanence of exploring and understanding microorganisms. A species of microorganism is a unique gene pool. The metabolism of microorganisms is an intricate life activity. In the long-term biological evolution, a complete and unified sensitive regulatory system has been formed. Depending on this regulatory system, various metabolic activities are strictly controlled. . Methodical and coordinated and adapts flexibly to circumstances. Different culture media, due to different media formulations, affect its growth and variation. [0003] Medium is an artificially prepared nutrient for the growth and maintenance of microorganisms, plants, and animal tiss...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
Inventor 王明刚孟宪斌
Owner QINGDAO RUNXIN WEIYE TECH & TRADE
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