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A kind of probiotics for chicken and preparation method thereof

A technology of micro-ecological preparations and seeds, applied in the field of preparation of microbial preparations, can solve the problems of increasing the risk of food allergies or intestinal inflammatory diseases, incomplete functions of single strains and compound preparations, and ineffective joint, coordinated and superimposed effects, etc. problems, achieve the effect of preventing drug-resistant pathogens and mixed infections, preventing intestinal diseases of livestock and poultry, and highlighting the effect of prevention

Active Publication Date: 2017-01-25
山东省健牧生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are more than 1,000 types of bacteria in the intestines of healthy people and other animals. These microbial flora play an important role in the formation of the intestinal immune system, and each microorganism is important for maintaining the body's digestion and vitamin metabolism and for the host. Your health is indispensable, if you change the composition of intestinal flora will increase the risk of food allergies or intestinal inflammatory diseases
Therefore, the single-bacteria preparation and the composite preparation have the following defects: 1. The functions of the single-strain and the composite preparation are not comprehensive, and the joint, coordination and superposition effects are not obvious; 2. The strains used in some single-strain and composite preparations are non-native bacteria , there is a risk of disease

Method used

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  • A kind of probiotics for chicken and preparation method thereof
  • A kind of probiotics for chicken and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 300ml of bean sprouts liquid, and then add 8g of fish meal peptone, 0.6g of cysteine ​​hydrochloride, 6g of soybean peptone, vitamin B 1 0.4g, 20g of beef extract, 2g of yeast extract powder, 0.5g of soluble starch, 10g of glucose, 25g of dipotassium hydrogen phosphate, and 10g of magnesium sulfate; then heat to 100°C to dissolve it completely, at this time all components are dissolved, and then stir Mix until uniform; then add water to make up 1000ml, and then adjust the pH value to 7.0; after aliquoting, sterilize at 121°C for 30 minutes to obtain the culture medium.

[0022] Dissect the SPF adult hen conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal content (intestinal content for short) and weigh it; then use the weight ratio of intestinal content:medium as 1:10 Add the intestinal contents into the culture medium, stir well and then filter to obtain the filtrate; inoculate the filtrate into the culture medium accordin...

Embodiment 2

[0024] Take 300ml of bean sprouts liquid, and then add 8g of fish meal peptone, 0.6g of cysteine ​​hydrochloride, 6g of soybean peptone, vitamin B 1 0.4g, 20g of beef extract, 2g of yeast extract powder, 0.5g of soluble starch, 10g of glucose, 25g of dipotassium hydrogen phosphate, and 10g of magnesium sulfate; then heat to 100°C to dissolve it completely, at this time all components are dissolved, and then stir Mix until uniform; then add water to make up 1000ml, and then adjust the pH value to 7.0; after aliquoting, sterilize at 121°C for 30 minutes to obtain the culture medium.

[0025] Dissect the SPF adult hen conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal content (referred to as the intestinal content) and weigh it; then use the weight ratio of intestinal content:medium as 1:9 Add the intestinal contents into the culture medium, stir well and then filter to obtain the filtrate; inoculate the filtrate in the culture medium ac...

Embodiment 3

[0027] Take 300ml of bean sprouts liquid, and then add 8g of fish meal peptone, 0.6g of cysteine ​​hydrochloride, 6g of soybean peptone, vitamin B 1 0.4g, 20g of beef extract, 2g of yeast extract powder, 0.5g of soluble starch, 10g of glucose, 25g of dipotassium hydrogen phosphate, and 10g of magnesium sulfate; then heat to 100°C to dissolve completely, at this time all components are dissolved, and then stir Mix until uniform; then add water to make up 1000ml, and then adjust the pH value to 7.0; after aliquoting, sterilize at 121°C for 30 minutes to obtain the culture medium.

[0028] Dissect the adult hen with SPF conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal contents (intestinal contents for short) and weigh them; Add the intestinal contents into the culture medium, stir thoroughly and then filter to obtain the filtrate; inoculate the filtrate into the culture medium at an inoculum size of 11%, then place it in an anaerobic c...

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Abstract

The invention relates to a chicken microecological preparation and a preparation method thereof and belongs to the technical field of preparation of microbial preparations. The chicken microecological preparation provided by the invention is prepared by diluting whole intestinal contents of a chicken with a culture medium and then filtering to obtain filtrate; further inoculating the filtrate into the culture medium, and culturing in an anaerobic culture system at the temperature of 40.5-41.5 DEG C for 35-37h to obtain a seed solution of a culture solution of intestinal bacteria; and finally inoculating the seed solution into a fermentation tank, filling nitrogen into the tank, keeping the pressure at 0.018-0.022MPa, keeping the pH at 6.8-7.0, and performing static culture at 40.5-41.5 DEG C for 46-50h. The microecological preparation is an all-weather, multi-bacterial species, union, coordination, overlapping and joint action biogenic compound preparation which has no toxicity, no side effects, no rejections, no pharmaceutical residues, no hormone residues and no production of drug resistance of bacteria. The content of effective living bacteria is not lower than 10 billion / ml; the effective period of biological activity of main probiotics is prolonged to 8 months at normal temperature.

Description

technical field [0001] The invention relates to a microecological preparation for chickens and a preparation method thereof, belonging to the technical field of preparation of microbial preparations. Background technique [0002] Microecological preparations, also known as probiotics, probiotics, live bacteria preparations, prebiotics, are beneficial microorganisms that are isolated and identified from animals or nature, or artificially constructed through bioengineering, and are made by special processes such as cultivation, fermentation, drying, and processing. Biological preparations or live bacterial preparations containing live bacteria and intended for animals. Animal microecological preparations in a broad sense include not only normal microbial members, namely prebiotics (bacteria), but also some preparations that can promote the growth and reproduction of normal microbiota, such as prebiotics such as oligosaccharides. In a narrow sense, probiotics refer to probioti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18A23K50/75C12R1/23C12R1/145C12R1/01
Inventor 张从敬黄迪海秦卓明王世荣刘霞张再辉徐秀荣盛晓丹
Owner 山东省健牧生物药业有限公司
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