Bioactive bacterial cellulose-zein composite film and preparation method thereof
A technology of zein and bacterial cellulose film, which is applied in the field of biomedical materials, can solve the problems of long time-consuming and low actual utility of bacterial cellulose composite film materials, and achieve rapid and efficient material preparation methods, enhanced solubility and stability Sexuality, the effect of prolonging the effective period of action
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] Step 1, preparation of bacterial cellulose membrane
[0042] Medium composition: 2.0% D-glucose, 0.5% peptone, 0.5% yeast extract, 0.115% citric acid, 0.27% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH5.0
[0043] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, and vibrate at 30°C for 12 hours, then inoculate 10% of the fermentation medium, shake well, and culture at 30°C for 5 days to obtain a bacterial cellulose film.
[0044] Step 2, purification of bacterial cellulose membrane
[0045] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in a NaOH solution with a concentration of 0.2mol / L, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, and boil it at 90°C for 30min , change the water, repeat 3 times, then soak it in distilled water until neutral to obtain the purified bacterial cellulose membrane;
[0046] Step 3, preparation of bacterial ce...
Embodiment 2
[0075] Step 1, preparation of bacterial cellulose membrane
[0076] Medium composition: 1.8% D-glucose, 0.45% peptone, 0.45% yeast extract, 0.120% citric acid, 0.25% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH4.8.
[0077] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 15%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.
[0078] Step 2, purification of bacterial cellulose membrane
[0079] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in 0.4mol / L NaOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, change the water , repeated 4 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;
[0080] Step 3, preparation of bacterial c...
Embodiment 3
[0112] Step 1, preparation of bacterial cellulose membrane
[0113] Medium composition: 2.2% D-glucose, 0.6% peptone, 0.6% yeast extract, 0.110% citric acid, 0.30% Na 2 HPO 4 12H 2 O, the rest is distilled water, pH 4.0.
[0114] Cultivation method: inoculate Acetobacter xylinum into the seed culture solution, shake and cultivate at 30°C for 12 hours, then inoculate the fermentation medium at a ratio of 12%, shake well, and culture at 30°C for 5 days to obtain bacterial cellulose membrane.
[0115] Step 2, purification of bacterial cellulose membrane
[0116] Take the bacterial cellulose membrane prepared above, wash it with clean water for 3 times, soak it in 0.2mol / L KOH solution, boil it at 90°C for 30min, take it out, then soak the membrane in distilled water, boil it at 90°C for 30min, change the water , repeated 5 times, and then soaked in distilled water until neutral to obtain the purified bacterial cellulose membrane;
[0117] Step 3, preparation of bacterial cel...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
quality score | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com