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Pearl enzymolysis solution and preparation method thereof

The technology of pearl enzymolysis solution and enzymolysis solution is applied in the field of biological enzymolysis, which can solve the problems of large molecular weight of polypeptide fragments and incomplete enzymolysis, and achieve the effects of high enzymolysis rate, rich variety and low cost.

Active Publication Date: 2015-01-07
SHANGHAI RONGSHEN BIOCHEM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

And the patent application CN1051394A whose title of invention is "new preparation method of water-soluble full-ingredient pearl powder series product" edible carboxylic acid or amino acid replaces the carbonate group of mineral matter in pearl, makes pearl hard protein separate out, then uses acid, enzyme double Several kinds of water-soluble full-range pearl products are made by hydrolysis, but there are certain deficiencies
[0004] Because pearl powder has not been completely hydrolyzed into components that can be easily absorbed by the human body, there are still some shortcomings, especially the shell keratin protein, one of its main components, has not been fully absorbed and fully utilized by the human body.
In order to solve this technical defect, the patent application No. 94112053.8 discloses that papain is used to enzymatically hydrolyze pearl powder to obtain amino acids and polypeptides that can be absorbed by the human body. In order to improve the efficiency of enzymatic hydrolysis and obtain more small molecule peptide products, this method was invented after repeated trials

Method used

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  • Pearl enzymolysis solution and preparation method thereof
  • Pearl enzymolysis solution and preparation method thereof
  • Pearl enzymolysis solution and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: a kind of pearl enzymolysis solution developed by the present invention, the raw material of this enzymolysis solution comprises pearl powder, volume concentration is 5% lactic acid solution, papaya peptidase, L-cysteine, ethylenediaminetetraacetic acid Disodium salt, distilled water, the mass ratio of the above components is 15:50:15:1:1:1000.

[0019] The raw materials taken by the present invention have no toxic and side effects, and the obtained enzymolyzed liquid has no toxicity. Drinking as a health drink and applying cosmetics to the skin has no side effects. The enzymolyzed pearl liquid is tested according to the drug toxicity test method of Shanghai Drug Inspection Institute. The acute toxicity LD50 test was carried out by intragastric administration of mice. The result is as follows:

[0020] Kunming hybrid mice, weighing 20 grams, were used and divided into male and female groups. 10 rats in each group, administered with the same dosage, and o...

Embodiment 2

[0021] Embodiment two: the raw material of pearl enzymolysis solution of the present invention comprises pearl powder, volume concentration is 10% lactic acid solution, papaya peptidase, L-cysteine, edetate disodium salt, distilled water, the above-mentioned composition The mass ratio is 30:100:10:3:3:1000

[0022] The method for preparing the above-mentioned pearl enzymatic hydrolysis solution includes the following steps, (1) preparing a lactic acid solution, the volume concentration of which is 10%;

[0023] (2) firstly add pearl powder to the lactic acid solution prepared in step (1) to carry out acidolysis reaction, centrifuge after the acidolysis reaction, the precipitate obtained is pearl shell keratin, then pearl shell keratin, papaya peptidase , L-cysteine, ethylenediaminetetraacetic acid diamine salt, and distilled water are mixed for enzymatic hydrolysis reaction. The mass ratio of sodium salt and distilled water is 30:100:10:3:3:1000, and the amino acid sequence o...

Embodiment 3

[0029] Embodiment three: the pearl enzymolysis liquid that the present invention develops, the raw material of this enzymolysis liquid comprises pearl powder, volume concentration is 7% lactic acid solution, papaya peptidase, L-cysteine, edetate disodium salt , distilled water, the mass ratio of the above components is 20:70:13:2:2:1000.

[0030] The method for preparing the above-mentioned pearl enzymatic hydrolysis solution comprises the following steps, (1) preparing a lactic acid solution, the volume concentration of which is 9%;

[0031] (2) firstly add pearl powder to the lactic acid solution prepared in step (1) to carry out acidolysis reaction, centrifuge after the acidolysis reaction, the precipitate obtained is pearl shell keratin, then pearl shell keratin, papaya peptidase , L-cysteine, ethylenediaminetetraacetic acid diamine salt, and distilled water are mixed for enzymatic hydrolysis reaction. The mass ratio of sodium salt to distilled water is 20:70:13:2:21000, ...

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Abstract

The invention discloses a pearl enzymolysis solution. The pearl enzymolysis solution comprises the raw materials, namely pearl powder, lactic acid, papaya peptidase, L-cysteine, ethylenediaminetetraacetic acid disodium salt and distilled water. The invention also discloses a method for preparing the pearl enzymolysis solution. The method comprises the following steps: (1) preparing a lactic acid solution; (2) carrying out enzymolysis reaction; and (3) centrifuging enzymolysis products and carrying out sterilization treatment on obtained supernate to obtain the pearl enzymolysis solution. By utilizing the method, the papaya peptidase is extracted from the papaya powder simply and conveniently with low cost and high purity, the pearl powder is subjected to enzymolysis by adopting the papaya peptidase, twenty amino acids and micro-molecule polypeptide substance with the molecular weight about 300-1000 can be obtained mainly, types of the pearl powder subjected to enzymolysis are enriched, enzymolysis of pearl can be carried out not only by using one single enzyme, and actual and efficient utilization of the pearl powder is further promoted.

Description

technical field [0001] The invention belongs to the technical field of biological enzymolysis, in particular to a pearl enzymolysis solution and a preparation method thereof. Background technique [0002] Pearl is a treasure in the motherland's medical treasure house. As early as the Han Dynasty's "Bielu of Famous Doctors" to the Ming Dynasty's "Compendium of Materia Medica", there are exact records of the efficacy of pearls. For a long time people have adopted the method of taking pearl powder for a long time to keep the skin lubricated and delicate, but because the main component in pearl powder is calcium carbonate and some other ingredients are not easy to be absorbed by the body. In particular, the shell keratin protein in pearls is not easy to decompose and absorb in the body. Therefore hindered the active effect of pearl powder and its special function greatly. [0003] With the continuous development of science and technology and the continuous improvement of peopl...

Claims

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Application Information

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IPC IPC(8): A61K8/98A61K8/44A61K8/64A23L2/38A23L1/29A61Q19/00A23L33/00
Inventor 丛峰松王定恩李朝阳
Owner SHANGHAI RONGSHEN BIOCHEM CO LTD
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