Preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in medium-pressure column

A technology of tyrosinase and fisetinase, applied in the directions of organic active ingredients, preparations for skin care, medical preparations containing active ingredients, etc., to achieve the effect of easy implementation, high purity, prevention and treatment of chronic diseases

Active Publication Date: 2015-01-07
陕西秦乔农林生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many evaluations have been made on the activity of flavonoids in sumac, the main purpose is not to develop natural tyrosinase inhibitors

Method used

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  • Preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in medium-pressure column
  • Preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in medium-pressure column
  • Preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in medium-pressure column

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step one, degreasing:

[0041] The lacquer wood flour was dried, crushed, and degreased with petroleum ether. The volume ratio of wood powder mass and petroleum ether was 1:15 g / mL, at a temperature of 30°C, extracted for 3 h, extracted 4 times, filtered to obtain a filter residue, dried, and degreased sumac powder;

[0042] The second step, extract:

[0043] Add 1 g raw material of sumac powder to 15 mL of 50% methanol aqueous solution or ethanol aqueous solution to extract 4 times, the extraction temperature is 60 ° C, the extraction time is 1 h, after extraction, filter, combine the filtrates, and concentrate in vacuum to obtain the crude extract of sumac;

[0044] The third step, extraction:

[0045] The extract obtained from the extract was extracted 3 times with ethyl acetate organic solvent, and the ethyl acetate layer was concentrated to obtain ethyl acetate extract;

[0046] The fourth step, medium pressure column purification:

[0047] The ethyl acetate ex...

Embodiment 2

[0053] Step one, degreasing:

[0054] The lacquer wood powder was dried, crushed, and degreased with petroleum ether, diethyl ether, n-hexane, pentane, and n-pentane organic solvents. The ratio of wood flour mass to organic solvent volume was 1:35 g / mL at a temperature of 80 °C for 1 h. 1 time, filter to obtain filter residue, dry to obtain defatted sumac powder;

[0055] The second step, extract:

[0056] Add 1 g raw material of sumac powder to 30 mL of 30% methanol aqueous solution or ethanol aqueous solution to extract 1-4 times, the extraction temperature is 680 ° C, the extraction time is 3 h, after extraction, filter, combine the filtrates, and vacuum concentrate to obtain the crude extract of sumac ;

[0057] The third step, extraction:

[0058] The extract obtained from the extract is extracted twice with an ethyl acetate organic solvent, and the ethyl acetate layer is concentrated to obtain an ethyl acetate extract;

[0059] The fourth step, medium pressure column...

Embodiment 3

[0065] Step one, degreasing:

[0066] The lacquer wood powder was dried, crushed, degreased with n-hexane, the volume ratio of wood powder mass and n-hexane was 1:20 g / mL, the temperature was 60°C, extracted for 2 h, extracted twice, filtered to obtain filter residue, dried to obtain degreased sumac powder;

[0067] The second step, extract:

[0068] Add 1 g raw material of lacquer tree powder to 20 mL of methanol aqueous solution or ethanol aqueous solution of different concentrations to extract 1-4 times, the extraction temperature is 60-80°C, and the extraction time is 1-3 h. After extraction, filter, combine the filtrates, and concentrate in vacuo to obtain Sumac crude extract;

[0069] The third step, extraction:

[0070] Extracting the extract obtained from the extract with ethyl acetate organic solvent for 2-5 times, concentrating the ethyl acetate layer to obtain the ethyl acetate extract;

[0071] The fourth step, medium pressure column purification:

[0072] The...

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Abstract

The invention relates to a preparation method of fisetin capable of inhibiting tyrosinase activity through rapid separation in a medium-pressure column, and belongs to the fields of development and applications of plant extract, natural drugs, and cosmetics. Fisetin with purity more than 95% can be obtained through the following steps: taking lacquer tree wood powder as the raw material, extracting the wood powder, and subjecting the extract to a middle-low pressure chromatogram and HPLC (high performance liquid chromatogram). The component obtained from lacquer tree can inhibit the tyrosinase activity efficiently, and the inhibition rate on the tyrosinase activity is greater than 95% when the concentration of the component is 150 [mu]g / mL or more. The preparation is simple and fast, and thus the provided method can be used to produce a natural tyrosinase inhibitor.

Description

technical field [0001] The invention relates to a preparation method for rapidly separating fisetin with tyrosinase-inhibiting activity by a medium-pressure column, and belongs to the technical fields of plant extracts, natural medicines and cosmetics. Background technique [0002] Sumac (Rhus verniciflua stokes) is a deciduous tree belonging to Anacardiaceae (Toxicodendron). China is the origin of sumac, and sumac is an important special-purpose economic forest in China. It is mainly distributed in Shaanxi, Sichuan, and Chongqing , Hubei, Guizhou, Gansu and Yunnan provinces. At present, the chemical utilization of lacquer is mainly concentrated on two aspects of lacquer and lacquer seeds, and there is little research on the active substances of lacquer wood. lacquer itself is an excellent medicinal wood , the leaves, flowers, roots, bark, fruit, dried lacquer and wood core of the sumac tree can be used as medicine, which has the effects of relieving cough, eliminating addic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07D311/40A61K31/352A61P17/00A61K8/49A61Q19/02
CPCA61K8/498A61K31/352C07D311/30C07D311/40
Inventor 王成章陈虹霞叶建中
Owner 陕西秦乔农林生物科技有限公司
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