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Culture medium for culturing chicken's normal intestinal bacteria flora and preparation method of culture medium

A technology of intestinal flora and culture medium, which is applied in the field of culture medium for cultivating native intestinal flora of chickens and its preparation, can solve problems such as failure to cultivate, achieve long biological activity period, fast reproduction speed, and preparation method simple effect

Active Publication Date: 2015-01-07
山东省健牧生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no culture medium that can cultivate a flora that is basically consistent with the proportion of bacillus cocci and the proportion of positive bacteria and negative bacteria in the original intestinal flora of chickens

Method used

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  • Culture medium for culturing chicken's normal intestinal bacteria flora and preparation method of culture medium
  • Culture medium for culturing chicken's normal intestinal bacteria flora and preparation method of culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Take 300ml of bean sprouts liquid, then add 8g of fish powder peptone, 0.6g of cysteine ​​hydrochloride, 6g of soybean peptone, vitamin B 1 0.4g, beef extract 20g, beef liver extract powder 10g, yeast extract powder 2g, soluble starch 0.5g, glucose 10g, dipotassium hydrogen phosphate 25g, magnesium sulfate 10g; then heat to 100°C to dissolve, all components are dissolved After that, stir again until it is evenly mixed; then add water to make up 1000ml, and then adjust the pH value to 7.0; after dispensing, sterilize at 121°C for 30 minutes to obtain the culture medium for cultivating chicken native intestinal flora of the present invention (referred to as culture medium).

[0022] Dissect the SPF adult hen conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal content (intestinal content for short) and weigh it; then use the weight ratio of intestinal content:medium as 1:10 Add the intestinal contents into the culture medium, sti...

Embodiment 2

[0024] Take 315ml of bean sprouts liquid, then add 8.4g of fish powder peptone, 0.63g of cysteine ​​hydrochloride, 6.3g of soybean peptone, vitamin B 1 0.42g, beef extract 21g, beef liver extract powder 10.5g, yeast extract powder 2.1g, soluble starch 0.525g, glucose 10.5g, dipotassium hydrogen phosphate 26.25g, magnesium sulfate 10.5g; then heated to 100°C to dissolve, After each component is dissolved, stir until evenly mixed; then add water to make up 1000ml, and then adjust the pH value to 7.0; after subpackaging, sterilize at 121°C for 30 minutes to obtain the chicken native intestinal flora of the present invention. culture medium (referred to as culture medium).

[0025] Dissect the SPF adult hen conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal content (intestinal content for short) and weigh it; then use the weight ratio of intestinal content:medium as 1:10 Add the intestinal contents into the culture medium, stir well and ...

Embodiment 3

[0027] Take bean sprouts liquid 285ml, then add fish meal peptone 7.6g, cysteine ​​hydrochloride 0.57g, soybean peptone 5.7g, vitamin B 1 0.38g, beef extract 19g, beef liver extract powder 9.5g, yeast extract powder 1.9g, soluble starch 0.525g, glucose 9.5g, dipotassium hydrogen phosphate 23.75g, magnesium sulfate 9.5g; then heated to 100°C to dissolve, After each component is dissolved, stir until evenly mixed; then add water to make up 1000ml, and then adjust the pH value to 7.0; after subpackaging, sterilize at 121°C for 30 minutes to obtain the chicken native intestinal flora of the present invention. culture medium (referred to as culture medium).

[0028] Dissect the SPF adult hen conforming to the GB / T17999-2008 standard in a sterile environment, take its whole intestinal content (intestinal content for short) and weigh it; then use the weight ratio of intestinal content:medium as 1:10 Add the intestinal contents into the culture medium, stir well and then filter to o...

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Abstract

The invention relates to a culture medium for culturing chicken's normal intestinal bacteria flora and a preparation method of the culture medium, and belongs to the technical field of microbiological culture medium preparation. A pH value of the culture medium is 6.8-7.1; the culture medium is mainly prepared from the following components: bean sprout liquid, fish meal peptone, cysteine hydrochloride, soybean peptone, vitamin B1, beef extract, beef liver extract powder, yeast extract powder, soluble starch, glucose, dipotassium phosphate and magnesium sulfate. The culture medium disclosed by the invention is in a liquid state and can be used for determining the content of effective viable bacteria, the content of probiotics, a proportion of bacillus to coccus, and a proportion of positive bacteria to negative bacteria in the bacterial flora obtained by culture as well as a biological activity expiration date of probiotics by virtue of the composition and the content of each component. Therefore, the bacterial flora which is almost consistent with the chicken's normal intestinal bacteria flora in the proportion of bacillus to coccus and the proportion of positive bacteria to negative bacteria can be cultured by adopting the components and the proportions of the culture medium.

Description

technical field [0001] The invention relates to a culture medium for cultivating native intestinal flora of chickens and a preparation method thereof, belonging to the technical field of microbial culture medium preparation. Background technique [0002] Medium is artificially formulated nutrients for the growth and maintenance of microorganisms, plant tissues and animal tissues, generally containing carbohydrates, nitrogenous substances, inorganic salts (including trace elements), auxins and water. Some media also contain antibiotics, pigments, hormones and serum. Although the growth and reproduction of all microorganisms requires the culture medium to contain carbon sources, nitrogen sources, inorganic salts, growth factors, water and energy, but different types of microorganisms have different requirements for nutrients. As far as the main types of microorganisms are concerned, there are bacteria, actinomycetes, yeasts, molds, protozoa, algae and viruses, and the cultu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/16C12N1/20C12R1/23C12R1/145C12R1/46
CPCC12N1/16C12N1/20
Inventor 秦卓明王世荣黄迪海张从敬张再辉刘霞徐秀荣盛晓丹
Owner 山东省健牧生物药业有限公司
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