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Preparation method of composite feed additive containing lipase and lactic acid bacteria

A compound feed, lipase technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of blank and less research on the mechanism of lipase action, and achieve the purpose of reducing emissions, improving the gastrointestinal environment, improving meat quality and improving meat quality. flavor effect

Inactive Publication Date: 2015-01-14
广东华农高科生物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, many scholars have conducted research on the application of lipase in animal nutrition, but most of these studies are animal nutrition experiments, and there are few studies on the mechanism of action of lipase, and most of them are on fish. The research on pigs is almost blank,

Method used

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  • Preparation method of composite feed additive containing lipase and lactic acid bacteria

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Step A: extracting and preparing feed lipase with high enzyme activity. The process route of its extraction is: filter the fermented liquid with plate and frame to remove bacteria, add thiamine to precipitate the filtrate after ultrafiltration and concentration, then filter to obtain enzyme sludge, and adopt low-temperature fluidized drying and granulation technology to dry the enzyme sludge , to obtain highly active dry enzyme granules.

[0029] Among them, the lipase is fermented by Candida, and its fermentation enzyme production process and lipase extraction are: 20m 3 In the fermentation tank, the ventilation rate is 0.4-0.8V / V / min, the stirring speed is 180r / min, the fermentation temperature is 35°C, cottonseed oil and olive oil (1:1) are added during the cultivation process to control the fermentation end point, and the pH value is Under the condition of 5.5~6.5, ferment for 72h, the mature fermentation enzyme activity is 4000U / mL. The fermented liquid is filter...

Embodiment 2

[0036] Step A: Candida is used for lipase, and its fermentation enzyme production process and lipase extraction are: 20m 3 The fermentation tank has a ventilation rate of 0.4-0.8V / V / min, a stirring speed of 180r / min, and a fermentation temperature of 35°C. During the cultivation process, cottonseed oil and olive oil (1:1) are added to control the fermentation end point, and the pH value is Under the condition of 5.5~6.5, ferment for 72h, the mature fermentation enzyme activity is 4000U / mL. The fermented liquid is filtered, concentrated by ultrafiltration, precipitated by ammonium sulfate, dried and granulated to obtain the finished product of granular alkaline lipase. 4 U / g.

[0037] Step B: preparing microencapsulated complex lactobacilli, specifically including the following aspects.

[0038] The activation method of Lactobacillus is as follows: take a small amount of Lactobacillus stored at -70°C and inoculate them in MRS liquid medium respectively, culture them staticall...

Embodiment 3

[0043] 1. Animals for testing: 180 weaned piglets at the age of 21 days were randomly divided into 2 treatment groups, each of which was repeated 5 times, and 18 piglets were each repeated. The test group of the prepared premix (adding 1.0kg / T feed). Group C is the test group using the premix prepared in Example 2 (1.0 kg / T feed added).

[0044] 2. The test period is 42 days.

[0045] 3. Measuring indicators: daily weight gain; feed-to-meat ratio; diarrhea rate; death rate.

[0046] 4. The test is shown in Table 1

[0047] Table 1

[0048] indicators

Group A

Group B

Group C

Initial weight(kg)

8.36±0.82 a

8.78±0.68 a

8.85±0.65 a

Weight at the end of the test (kg)

25.33±3.21 a

26.38±3.82 a

26.58±3.67 a

Daily weight gain (kg / d)

0.404±0.06 a

0.419±0.09 a

0.424±0.08 a

material to weight ratio

1.56±0.12 a

1.50±0.15 a

1.45±0.12 a

Diarrhea rate (%)

4.55±0.66 a

2.82±0.45 a...

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Abstract

The invention provides a composite feed additive containing lipase and lactic acid bacteria and a preparation method of the composite feed additive. The composite feed additive comprises the following components by weight percentage: 70%-85% of feed lipase, 10%-20% of lactic acid bacteria, 1%-2% of potassium chloride, 1%-2% of sodium citrate, 1%-2% of calcium lactate, 1%-2% of magnesium sulfate and 1%-2% of anhydrous glucose. The composite feed additive provided by the invention has the following beneficial effects 1) the composite feed additive can promote digestion and absorption of fat against insufficient secretion of endogenous lipase in a young animal (suckling pig); 2) the composite feed additive can reduce the dependence on antibiotics in the formula in control of the diarrhea rate, further improve daily weight gain and intake of the young animal and improve the utilization rate of a feed against nutritional diarrhea; 3) the composite feed additive can bring about hair brightness and improve meat quality and local flavor and increase comprehensive benefits of farming; 4) the composite feed additive can improve the gastrointestinal environment, enhance the immunity of an animal organism and reduce gastrointestinal diseases; and 5) the composite feed additive can reduce fecal odor and improve a farming environment against emission of ammonia gas.

Description

technical field [0001] The invention relates to a feed additive and a preparation method thereof, in particular to a highly targeted and functional feed additive developed according to the characteristics of the gastrointestinal tract of animals at different stages, focusing on improving fat digestibility and reducing diarrhea rate. Composite product using lipase and lactic acid bacteria and its preparation method. Background technique [0002] With the rapid development of the modern pig industry, the production performance of pigs is getting higher and higher. How to formulate a diet with a higher nutritional level in an effective formula capacity has put forward higher requirements for feed ingredients. Among many feed nutrients, energy is particularly common and important. For sows at the peak of lactation, when the energy provided by the diet cannot meet the needs of maintaining and lactating, the fat tissue of the body will actively decompose, and even mobilize body p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/165A23K1/175
Inventor 李美娣武力涂玉蓉王昌库
Owner 广东华农高科生物药业有限公司
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