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Microbial whole-cell luminescent reporting sensor for monitoring Hg<2+> pollution and kit prepared thereby and application

A kit and a technology for monitoring the environment, which are applied in the field of microbial whole-cell luminescent reporter sensor kits, can solve the problems of merR-Pt promoters and reporter gene patents and reports, etc., and achieve strong specificity and adaptability, Reliable results and easy detection

Inactive Publication Date: 2015-01-28
NANJING NORMAL UNIVERSITY
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  • Abstract
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  • Claims
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Problems solved by technology

[0007] At present, there are no patents and reports on the construction of a stable recombinant merR-Pt promoter and reporter gene and its use in China

Method used

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  • Microbial whole-cell luminescent reporting sensor for monitoring Hg&lt;2+&gt; pollution and kit prepared thereby and application
  • Microbial whole-cell luminescent reporting sensor for monitoring Hg&lt;2+&gt; pollution and kit prepared thereby and application
  • Microbial whole-cell luminescent reporting sensor for monitoring Hg&lt;2+&gt; pollution and kit prepared thereby and application

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Embodiment Construction

[0025] The present invention will be further described below in conjunction with the accompanying drawings.

[0026] The specific steps of the invention are as follows: constructing a recombination system containing merR-Pt promoter and egfp reporter gene; transforming the system into highly mercury-resistant bacterial cells through plasmids. The bacteria were embedded and fixed in a certain buffer system, and tested by Hg 2+ The induction of green fluorescent protein can detect Hg in the environment according to the change of fluorescence intensity. 2+ pollute.

[0027] 1. Experimental materials

[0028] 1. The strains and plasmid sources involved in the present invention are shown in Table 1.

[0029] Table 1. Strains and plasmids

[0030]

[0031] AP r : Ampicillin resistance, Cm r : Chloramphenicol resistance, Km r : Kanamycin resistance

[0032] NJZW Corp.: Nanjing Zun Microbial Technology Co., Ltd.

[0033] 2. Biochemical reagents

[0034] 1) XhoⅠ, NotⅠ, Eco...

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Abstract

The invention relates to a microbial whole-cell luminescent reporting sensor for monitoring Hg<2+> pollution and a kit prepared thereby and an application. The microbial whole-cell luminescent reporting sensor for monitoring Hg<2+> pollution is a whole-cell luminescent sensor engineering bacterium which is constructed by importing a merR-Pt-containing anti-mercury gene regulating region and egfp genes into a host which is a highly anti-mercury bacterium. The kit comprises an immobilized cell culture which contains the bacterium and a special buffer which is suitable for testing. The sensor kit can be used for specifically and sensitively monitoring pollution of heavy metal Hg<2+> which is the strongest in toxicity in a soil, river, lake, underground water and municipal wastewater treatment system. Compared with the prior art, the microbial whole-cell sensor provided by the invention is strong in specificity and adaptability, convenient to use and high in monitoring sensitivity and can be used for high throughput online monitoring.

Description

technical field [0001] The invention discloses a microbial whole-cell luminescent reporter sensor kit containing a mercury-resistant gene promoter and a reporter gene, as well as production, use methods and applications. Background technique [0002] Mercury is a chemical substance with severe physiological toxicity. Due to its persistence, easy migration and high bioaccumulation, it has become one of the most concerned environmental pollutants in the world. According to the latest statistics, the current mercury content in water, soil, and air has tripled over the past few years. As we all know, mercury pollution is extremely harmful to human beings. The human body cannot excrete trace amounts of mercury accumulated in the food chain or other ways through its own metabolism. The accumulation of trace amounts of mercury will directly lead to heart, liver, thyroid and other diseases, and even lead to nervous system disorders and chronic mercury poisoning. In industrializati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/78C12N1/21G01N21/64C12R1/40
Inventor 何伟戴传超
Owner NANJING NORMAL UNIVERSITY
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