Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Catalase fluorescence detection method based on gold nanocluster probe

A gold nanocluster and catalase technology, applied in analytical chemistry and nanometer fields, can solve the problems of poor repeatability, insufficient precision, complicated operation, etc., and achieve the effects of high sensitivity, fast preparation process and good specificity

Inactive Publication Date: 2016-11-02
FUJIAN MEDICAL UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For these methods, some require expensive instruments, some require special reagents, some are complicated to operate, and some methods themselves have poor repeatability and insufficient precision.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Catalase fluorescence detection method based on gold nanocluster probe
  • Catalase fluorescence detection method based on gold nanocluster probe
  • Catalase fluorescence detection method based on gold nanocluster probe

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0033] Add 0.6 mL of 0.5 mol / L sodium hydroxide solution and 0.4 mL of 0.02 g / L chloroauric acid solution to 4 mL of 0.08 mol / L N-acetyl-L-cysteine ​​solution , mixed well, and placed in a 37°C constant temperature water bath to react for 2.5 h. After the reaction, the reaction solution was purified with a dialysis bag with a molecular weight cut off of 3500. The obtained gold nanocluster solution is colorless under visible light, and produces strong red fluorescence under ultraviolet light irradiation. Stored in the dark at 4°C, it can remain relatively stable for at least one month.

example 2

[0035] 0.025 ml of catalase solution with a concentration of 0.5 U / mL (containing HEPES——N-(2-hydroxyethyl)piperazine-N'-2-ethanesulfonic acid, concentration 20 mmol / L, pH=7.4 ) into 0.175 ml of a hydrogen peroxide solution with a concentration of 10 μmol / L (containing HEPES, a concentration of 20 mmol / L, pH=7.4), and reacted at 25°C for 90 minutes. 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ The solution (containing 40 mmol / L sulfuric acid) and 0.2 ml of the gold nanocluster solution prepared in Example 1 were sequentially added to the above reaction solution, shaken well and placed in a 25°C water bath for 10 minutes to react. Observed under ultraviolet light, the red fluorescence of the gold nanocluster solution without catalase was quenched after the Fenton reaction ( figure 1 A) in A), while the gold nanocluster solution recovered red fluorescence after adding catalase reaction ( figure 1 in B). figure 2 A gold nanocluster solution without adding catalase ( ...

example 3

[0037] Add 0.025 ml of 0.5 U / mL catalase solution (HEPES, 20 mmol / L pH=7.4) to 0.175 ml of 10 μmol / L hydrogen peroxide solution (HEPES, 20 mmol / L pH=7.4) in 25°C for 15-130 minutes. 0.05 ml of Fe with a concentration of 0.9 mmol / L 2+ The solution (containing 40 mmol / L sulfuric acid) and 0.2 ml of the gold nanocluster solution prepared in Example 1 were sequentially added to the above reaction solution, shaken well and placed in a 25°C water bath for 10 minutes to react. Such as image 3 As shown, after catalase catalyzed reaction for 90 minutes, the fluorescence intensity value F 650 The change is basically stable, so the catalase catalyzed reaction time is selected as 90 minutes.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a catalase fluorescence assay method based on a gold nano-cluster probe, which relates to a catalase assay using a gold nano-cluster protected by N-acetyl-L-cysteine ​​as a fluorescent probe The method is characterized in that Fe2+ is used to catalyze H2O2 to produce hydroxyl radicals to quench the fluorescence of gold nanoclusters, while catalase can catalyze the decomposition of H2O2 to generate H2O and O2, which inhibits the quenching of gold nanocluster fluorescence, thereby expressing The change of the characteristic of fluorescence emission spectrum can be used for the detection of catalase. In the range of 0.01-0.3 U / mL, the change value of fluorescence intensity ΔF650 had a linear relationship with the concentration of catalase, and the detection limit was 0.002 U / mL. The invention has high sensitivity and good reproducibility, and can be used for the determination of catalase in food, industry, environment and life systems.

Description

technical field [0001] The invention relates to a method for measuring catalase using N-acetyl-L-cysteine-protected gold nanoclusters as fluorescent probes, and belongs to the fields of analytical chemistry and nanotechnology. Background technique [0002] Catalase (also known as catalase) is an oxidoreductase widely present in biological tissues. Catalase is a tetrameric heminase consisting of four subunits arranged in the same tetrahedron, each subunit is 60,000 g / mol, and each molecule contains four haem groups Group, the molecular weight is around 240,000. The accumulation of reactive oxygen species and free radicals in the organism will lead to membrane lipid peroxidation, which will cause the metabolism disorder of the organism itself. Multiple enzymatic and non-enzymatic reactions can produce hydrogen peroxide (H 2 o 2 ), H 2 o 2 It is a precursor of active oxygen with toxic effects. The main function of catalase is to catalyze H 2 o 2 Decompose into water an...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 陈伟邓豪华彭花萍兰青刘爱林
Owner FUJIAN MEDICAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com