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Application of cationic polymer to prepare nanometer immunologic adjuvant and preparation method

A technology of cationic polymers and immune adjuvants, which is applied in the field of immunology, can solve the problems of restricting widespread use, and achieve uniform appearance, simple synthesis, and good reproducibility

Inactive Publication Date: 2015-03-04
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Freund's adjuvant used in the laboratory has limited widespread use due to its side effects

Method used

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  • Application of cationic polymer to prepare nanometer immunologic adjuvant and preparation method
  • Application of cationic polymer to prepare nanometer immunologic adjuvant and preparation method
  • Application of cationic polymer to prepare nanometer immunologic adjuvant and preparation method

Examples

Experimental program
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Effect test

Embodiment 1

[0027] Add 10g of anhydrous MPEG (molecular weight MW=5000Da) into a three-neck flask, weigh 4.8g of caprolactone, add 0.5% w / w stannous octoate dropwise, heat the oil bath to 120°C, stir magnetically and under nitrogen protection React for 4 hours, cool in vacuum, dissolve the reaction mixture with dichloromethane, repeatedly precipitate and wash with pre-cooled petroleum ether, purify, and identify the molecular weight of MPEG-PCL by NMR; weigh 10 g of dry MPEG5000-PCL2000 and wash with anhydrous 100 mL Dissolve dichloromethane, add 5 drops of triethylamine in advance, add 0.16g of acryloyl dropwise, and react for 4 hours at 35°C under the condition of anhydrous and nitrogen protection, and the synthetic product will be precipitated by petroleum ether; = MPEG-PCL with C-double bond is dissolved in dichloromethane, and excess (molecular weight MW=2000Da) polyethyleneimine (PEI) dichloromethane solution (to ensure excess PEI) is added dropwise, at 45 ° C, magnetic Under the co...

Embodiment 2

[0029] Add 10g of anhydrous MPEG (molecular weight MW=2000Da) into a three-neck flask, weigh 36g of caprolactone, add 0.5% w / w stannous octoate dropwise, heat the oil bath to 130°C, stir magnetically and react under nitrogen protection Cool in vacuum for 6 hours, dissolve the reaction mixture with dichloromethane, repeatedly precipitate and wash with pre-cooled petroleum ether, purify, and identify the molecular weight of MPEG-PCL by NMR; weigh 10 g of dry MPEG2000-PCL6000 and wash it with anhydrous 100 mL di Dissolve methyl chloride, add 5 drops of triethylamine in advance, add 0.16g of acryloyl dropwise, react at 35°C for 6 hours under the condition of anhydrous and nitrogen protection, and precipitate the synthetic product with petroleum ether; Dissolve C-double bond MPEG-PCL in dichloromethane, drop excess (molecular weight MW=2000Da) polyethyleneimine (PEI) in dichloromethane solution (to ensure excess PEI), at 45 ° C, magnetic stirring Under the following conditions, rea...

Embodiment 3

[0031] Add 10g of anhydrous MPEG (molecular weight MW=2000Da) into a three-necked flask, weigh 12g of caprolactone, add 0.5% w / w stannous octoate dropwise, heat the oil bath to 160°C, stir magnetically and react under nitrogen protection Cool in vacuum for 12 hours, dissolve the reaction mixture with dichloromethane, repeatedly precipitate and wash with pre-cooled petroleum ether, purify, and identify the molecular weight of MPEG-PCL by NMR; weigh 10 g of dry MPEG2000-PCL2000 and wash with anhydrous 100 mL di Dissolve methyl chloride, add 5 drops of triethylamine in advance, add 0.16g of acryloyl drop by drop, react at 35°C for 8 hours under the condition of anhydrous and nitrogen protection, and synthesize the product by petroleum ether precipitation; Dissolve C-double bond MPEG-PCL in dichloromethane, drop excess (molecular weight MW=2000Da) polyethyleneimine (PEI) in dichloromethane solution (to ensure excess PEI), at 45°C, magnetic stirring Under the following conditions, ...

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Abstract

The invention relates to application of a cationic polymer to prepare a nanometer immunologic adjuvant and a preparation method. Polyethylene glycol-polycaprolactone is employed for synthesizing a scaffold, and the scaffold is combined with polyethyleneimine for forming a cationic nanometer micelle, and physical attraction of positive and negative charges is utilized for finishing surface adsorption on a protein antigen, so that the cationic nanometer micelle-antigen particle composite system is prepared. The nanometer immunologic adjuvant is simple in synthesis, low in cost, simple in operation, good in reappearance, capability of realizing immunopotentiation and good in biocompatibility. The preparation method of the nanometer immunologic adjuvant is simple and easy to operate, the cost is low, and the prepared nanometer micelle is stably distributed in a water phase, is uniform in morphology and low in toxicity, and is capable of using simple electrostatic combination to finish loading and transmitting of an antibody, and enhancing immune response of body to an antigen.

Description

technical field [0001] The invention relates to the field of immunology, in particular to the application and preparation method of a cationic polymer as a nano immune adjuvant. Background technique [0002] With the continuous development of biotechnology, the safety of inactivated vaccines has been greatly improved, but when DNA or polypeptides developed based on recombinant antigens or purified pathogenic subunits are used as vaccines, the immunogenicity is very weak, which limits this. For the use of similar vaccines, immune adjuvants are required to improve the effect of humoral or cellular immune responses to such antigens. Traditional immune adjuvants are highly toxic, and it is urgent to develop safer and more effective new immune adjuvants to improve the immune effect of a new generation of biological vaccines. [0003] Immune adjuvants, also known as non-specific immune proliferators, are not antigenic in themselves. When injected together with the antigen or inje...

Claims

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Application Information

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IPC IPC(8): A61K39/39
Inventor 师帅罗资超李星熠陈浩
Owner WENZHOU MEDICAL UNIV
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