A method for preparing sulforaphane by utilizing aruginin

Abstract

The invention belongs to the field of biological chemistry, and particularly relates to a method for preparing sulforaphane by using roquette seed glycosides. The method comprises the following steps: oxidizing roquette seed glycosides to prepare 4-methyl-R-sulfinyl butyl glucosinolate by using a peroxidase-oxidase reaction system; hydrolyzing 4-methyl-R-sulfinyl butyl glucosinolate to prepare sulforaphane by using myrosinase. The defects of an existing preparation method that the raw material cost is high, the yield of the sulforaphane is low, the process is complicated, and large-scale production is difficult to achieve are overcome. Cheap and available roquette seeds, flowers, stems or leaves are adopted as raw materials; the raw material cost is 1 / 6 of that when broccoli is adopted as the raw material; the yield of the finally obtained sulforaphane is four times of that when the broccoli is adopted as the raw material; and the sulforaphane is high in purity, low in cost, high in yield, and extremely suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of biochemistry, and in particular relates to a method for preparing sulforaphane by utilizing aruginin. Background technique [0002] At present, with the improvement of people's living standards, the incidence of cancer has increased significantly, seriously affecting human life and health. Eating a lot of fruits and vegetables can significantly inhibit or slow down the occurrence of cancer. Vegetables and fruits contain a large number of active ingredients, such as lycopene in tomatoes, soybean isoflavones in soybeans, ellagic acid in fruits, and isothiocyanates and indoles in cruciferous vegetables. It has been proved to have outstanding anti-cancer and anti-cancer effects. [0003] Sulforaphane (sulforaphane, 4-methyl-sulfinyl butyl isothiocyanate) is currently recognized as one of the natural products with the best anti-cancer and anti-cancer effects, and is the strongest ability to induce type II detoxificati...

AI Technical Summary

Problems solved by technology

The chemical method adopts the method of stereochiral synthesis, which has complex experiments, many reaction steps, many by-products, high toxicity, low yield, high cost of raw materials, difficult separation and other shortcomings, making it difficult to achieve large-scale production

[0005] The natural product extraction method first extracts 4-methyl-R-sulfinylbutyl glucosinolate (R-glucoraphanin) in cruciferous plants such as broccoli seeds, and then uses myrosinase to hydrolyze 4-methyl-R - sulfinyl butyl glucosinolate (R-glucoraphanin) to get R-sulforaphane (4-methyl-R-sulfinyl butyl isothiocyanate), but due to the high cost of broccoli seeds , and the content of 4-methyl-R-sulfinyl butyl glucosinolate is low, the obtained sulforaphane products are few, and can only be produced on a laboratory scale

The Chinese invention patent application with the application number 200910037363.1 discloses a method for extracting multifunctional sulforaphane from broccoli sprouts. This method needs to consider the growth time of the sprouts and pretreat the sprouts. The process is complicated , low yield, high cost

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