Method for measuring nucleotide content in dairy product

A determination method and nucleotide technology, which are applied in the field of determination of nucleotide content in dairy products, can solve problems such as the lack of suitable methods for samples, and achieve the effects of being easy to master and easy to operate.

Inactive Publication Date: 2015-03-04
西安莹朴生物科技股份有限公司
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection methods currently reported mainly include microbiological method, ultraviolet spectrophotometry, high performance liquid chromatography, etc.,

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for measuring nucleotide content in dairy product
  • Method for measuring nucleotide content in dairy product

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example one:

[0018] (1) Preparation of standard solution: prepare stock solutions with GMP, UMP, GMP, IMP, and AMP concentrations of 1 mg / ml each; then prepare a series of standard concentrations of 10.0, 20.0, 30.0, 40.0, and 50.0 μg / ml. The injection volume of the solution is 10μl, and it is passed through a high performance liquid chromatograph on Dima C18 column, 5μm 4.6*200mm, column temperature 25℃, mobile phase flow rate is 1.0ml / min, UV detector wavelength is 254nm, mobile phase is Under the condition of 0.01mol / L potassium dihydrogen phosphate 960ml + 0.1mol / L dipotassium hydrogen phosphate 40mL mixture, the relationship curve and correlation coefficient of peak height and concentration are measured.

[0019] Thus, the peak height-concentration curve is shown in Table-1.

[0020] Table-1 Standard Curve

[0021]

[0022] According to Table-1, the regression equation of the five nucleotide standard curves can be calculated as:

[0023] CMP:Y=0.1754+0.0022X, the correla...

Example Embodiment

[0036] Example 2: Recovery rate and reproducibility test

[0037] (1) The determination method of the present invention performs 6 repeated determinations, and the resultant recovery rate is between 92%-97%, and the average recovery rate is 94.6%, and its accuracy can meet the requirements for determining infant formula milk powder.

[0038] (2) The determination method of the present invention is used to determine 8 parallel samples, and the result statistics show that the repeatability of the determination results is good, and the coefficient of variation is 1.79%, which can meet the requirement of determining the content of the glycidyl acid in infant formula milk powder.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a method for measuring nucleotide content in a dairy product and belongs to the field of food additives and detection methods thereof. The method comprises the following steps: (1), preparing stock solutions of GMP, UMP, CMP, IMP, AMP, wherein the concentrations of the stock solutions are 1 mg/ml, and preparing into solutions with the series normal concentrations of 10.0 [mu]g/ml, 20.0 [mu]g/ml, 30.0 [mu]g/ml, 40.0 [mu]g/ml and 50.0 [mu]g/ml, wherein the sample size is 10 [mu]l, measuring the peak heights of the solutions through a liquid chromatograph and calculating a regression equation; (2), weighing 10.0 g of a sample, dissolving the sample with 20 ml of warm water, putting into an ultrasonic bath for oscillation for 10 min, then taking out, transferring into a 50-ml volumetric flask, adding acetic acid for protein precipitation, after diluting, uniformly mixing, filtering through filtering paper, filtering through a filtering membrane, and measuring the peak height of the sample through the high performance liquid chromatograph; (3), according to a standard curve equation and the peak height value of the sample, obtaining the nucleotide content in the dairy product. According to the invention, the liquid chromatography is adopted to measure the nucleotide content in an infant formula milk powder, the method is simple and convenient to operate, the measurement precision and accuracy can meet analysis requirements of the dairy product, and the method can be used as a normal inspection method.

Description

technical field [0001] The invention relates to a method for measuring nucleotide content in dairy products, and belongs to the field of food additives and detection methods thereof. Background technique [0002] Nucleotides are the building blocks of nucleic acids and are currently used in dairy products primarily in infant formula. There are five main kinds of nucleotides added as additives --- cytosine nucleoside (CMP), uridine nucleoside (UMP), adenosine nucleoside (AMP), guanosine nucleoside (GMP), hypoxanthine nucleus Glycosides (IMPs). In addition, it can also be added to beverages, baked goods, and various main and non-staple foods and beverages. The main physiological function of nucleotides is to widely participate in the energy metabolism and protein synthesis of the human body, and to increase the activity of various enzymes, activate cell functions, promote antibody formation, and enhance immune function. They have a good health care effect on the human body,...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N30/02
Inventor 李勇刘文斌
Owner 西安莹朴生物科技股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap