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Method for preparing total ginsenosides by enzyme hydrolysis assisted by high-voltage pulsed electric field

A technology of high-voltage pulsed electric field and total ginsenosides, which is applied in the field of preparation of total ginsenosides, can solve the problems of high extraction temperature and long extraction time, and achieve the effects of high extraction rate, short extraction time and simple process conditions

Active Publication Date: 2018-03-16
CHANGCHUN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to propose a method for preparing total ginsenosides by enzymatic hydrolysis assisted by a high-voltage pulsed electric field, so as to overcome the disadvantages of high extraction temperature and long extraction time in the current traditional extraction method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The present invention uses high-voltage pulsed electric field assisted enzymatic hydrolysis to prepare total ginsenosides. Take 3g ginseng powder sample, add 50mL distilled water, stir evenly with magnetic stirrer, adjust pH to 5.0 with glacial acetic acid, add 1% β-glucosidase solution, substrate The concentration is 0.5mg / mL, enzymatically hydrolyzed under the action of PEF, the electric field intensity is 10kV / cm, and the number of pulses is 6. The reaction solution is extracted with water-saturated n-butanol, centrifuged, and the supernatant is evaporated to remove the solvent. The analysis yield of ginsenoside Rd was 2.36%.

Embodiment 2

[0030] The present invention uses high-voltage pulsed electric field assisted enzymatic hydrolysis to prepare total ginsenosides. Take 3g ginseng powder sample, add 50mL distilled water, stir evenly with magnetic stirrer, adjust pH to 5.0 with glacial acetic acid, add 1% β-glucosidase solution, substrate The concentration is 1.0mg / mL, enzymatically digested under the action of PEF, the electric field intensity is 10kV / cm, and the number of pulses is 6. The reaction solution is extracted with water-saturated n-butanol, centrifuged, and the supernatant is evaporated to remove the solvent. The analysis yield of ginsenoside Rd was 3.43%.

Embodiment 3

[0032] The present invention uses high-voltage pulsed electric field assisted enzymatic hydrolysis to prepare total ginsenosides. Take 3g ginseng powder sample, add 50mL distilled water, stir evenly with a magnetic stirrer, adjust the pH value to 5.0 with glacial acetic acid, add 2% β-glucosidase solution, substrate The concentration is 1.0mg / mL, enzymatically digested under the action of PEF, the electric field intensity is 10kV / cm, and the number of pulses is 8. The reaction solution was extracted with water-saturated n-butanol, centrifuged, and the supernatant was volatilized to remove the solvent. The yield of ginsenoside Rd was 3.52% analyzed by high performance liquid chromatography.

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PUM

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Abstract

The invention discloses a method for preparation of total saponins of panax ginseng by high-voltage pulse electric field assisted enzyme hydrolysis, and the method is as follows: taking 3g of panax ginseng dry powder, adding 50mL of distilled water; adding 1-3% beta-glucosidase liquid; performing enzyme hydrolysis in high-voltage pulse electric field with the electric field strength of 5-25kV / cm, wherein processed pulse number is 6-12; after the completion of the reaction, heating at 100 DEG C for 5min for passivating; using water saturated butanol for extraction reaction liquid, centrifugally separating, taking supernatant; performing vacuum rotary evaporation on extract of the total saponins of panax ginseng at 55 DEG, and dissolving a sample in methanol. Due to the introduction of high-voltage pulse electric field in the enzyme hydrolysis reaction process, the enzyme preparation activity is improved, the enzyme hydrolysis reaction is accelerated, and dissolution of the total saponin in the panax ginseng can be fast and efficiently promoted.

Description

Technical field [0001] The invention relates to a method for preparing total ginsenosides, in particular to a method for preparing total ginsenosides by high-voltage pulsed electric field assisted enzymatic hydrolysis. Background technique [0002] Ginseng (Panax ginseng C.A. Meyer) is a plant of the genus Araliaceae and is a traditional precious Chinese medicine. Ginseng has the functions of nourishing vitality, nourishing the spleen and lungs, promoting body fluid and quenching thirst, soothe the nerves and increase intelligence. Ginseng contains ginsenosides, volatile oils, organic acids, esters and polysaccharides and other active ingredients, among which ginsenosides are the main active ingredients of ginseng. At present, more than 40 ginsenoside monomers have been isolated from ginseng. [0003] Ginseng saponins are plant glycosides composed of aglycones and sugar bases, which are widely present in the roots, stems, leaves and flowers of ginseng. Ginsenosides are all trite...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/258
CPCA61K36/258A61K2236/19A61K2236/333A61K2236/51
Inventor 卢丞文殷涌光
Owner CHANGCHUN NORMAL UNIVERSITY