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Artificial skin prepared by collagen and polymer material through ultrasonic treatment

A technology of artificial skin and collagen, applied in the field of artificial skin, can solve the problems of increased acidity of surrounding tissue, insufficient strength, increase of connective tissue repair and regeneration, etc., and achieve the effect of simple preparation process, short production cycle and long storage time

Inactive Publication Date: 2015-03-25
江苏德威兰医疗器械股份有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Biodegradable materials such as glycolide are degradable and metabolizable, but if the molecular weight is small, the strength is not enough, and the molecular weight is large, it is difficult to dissolve in water, and it will degrade when dissolved, which will affect the mechanical strength of the material, and its degraded products will make it Increased acidity of surrounding tissue, aseptic inflammation
Collagen is an important component of artificial skin, a nutrient matrix for cell differentiation and growth, and can increase the repair and regeneration of connective tissue, but its disadvantages are inelasticity and low strength

Method used

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  • Artificial skin prepared by collagen and polymer material through ultrasonic treatment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Prepare artificial skin, its step comprises:

[0022] Take the bovine tendon material, clean it, cut it into small pieces, soak it with a mixed aqueous solution of 0.05% peracetic acid and 1% ethanol for 10 minutes, and use absolute ethanol to soak the bovine tendon material once at room temperature. First soak for 3 hours; fully mash the soaked material, soak in 0.2% pepsin solution for 12 hours for soaking treatment, the mass volume ratio of material to solution is 1:20 (g / ml); add 30% concentrated brine Carry out salting out. Centrifuge at 4000g for 30min. The precipitate was dissolved in 0.1% acetic acid, placed in a dialysis bag and dialyzed against 1 mol / L HCL for 5 hours. Phosphate buffer was used to adjust the pH value of the collagen solution system to 5.0, and the final concentration of the collagen solution was 0.2%. The solution was placed in an ultrasonic oscillator, and the temperature was adjusted to 20° C. and the frequency was 20 Hz. Then use sodium ...

Embodiment 2

[0024] Prepare artificial skin, its step comprises:

[0025] Take the bovine tendon material, clean it up, and cut it into small pieces, soak it with a mixed aqueous solution of 5% peracetic acid and 15% ethanol for 60 minutes, and use sodium carbonate solution to soak the bovine tendon material at room temperature for 5 times. First soak for 3 hours; fully mash the soaked material, soak in 5% pepsin solution for 48 hours for soaking treatment, the mass volume ratio of material to solution is 1:50 (g / ml); add 50% concentrated brine Carry out salting out. Centrifuge at 5000g for 50min. The precipitate was dissolved in 0.2% acetic acid, placed in a dialysis bag and dialyzed against 2mol / L HCL for 15 hours. Phosphate buffer solution was used to adjust the pH value of the collagen solution system to 6.5, the final concentration of the collagen solution was 1%, and placed in an ultrasonic oscillator, the temperature was adjusted to 25° C., and the frequency was 60 Hz. Then use s...

Embodiment 3

[0027] Prepare artificial skin, its step comprises:

[0028]Take the cartilage, clean it, cut it into small pieces, soak it in 0.1% sodium hydroxide solution for 4 hours, use methanol to soak the cartilage once at room temperature, and soak it for 3 hours each time; mash the soaked material fully, 0.2% pepsin solution was used for immersion treatment for 12 hours, and the mass volume ratio of material and solution was 1:20 (g / ml); 30% concentrated brine was added for salting out. Centrifuge at 4000g for 30min. The precipitate was dissolved in 0.1% acetic acid, placed in a dialysis bag and dialyzed against 1 mol / L HCL for 5 hours. Phosphate buffer was used to adjust the pH value of the collagen solution system to 5.0, and the final concentration of the collagen solution was 0.2%. The solution was placed in an ultrasonic oscillator, and the temperature was adjusted to 20° C. and the frequency was 20 Hz. Then use sodium hydroxide solution to adjust the pH value to 7.0, place it...

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Abstract

The invention belongs to the technical field of biological materials of tissue engineering and specifically relates to an artificial skin prepared by collagen and a polymer material through ultrasonic treatment. The preparation method of the artificial skin comprises the following steps of performing washing, cutting, sterilization treatment, degreasing treatment, tissue mashing treatment, acidic enzymolysis, salting-out and dialysis treatment, defoaming treatment, crosslinking treatment, treatment by a modifier, ultrasonic treatment, freeze-drying and vacuum drying on a tendon or dermis tissue. The artificial skin provided by the invention can not only protect a wound as a temporary wound cover for a human body, but also repair the wound by permanently retaining growth of a dermal scaffold, thereby overcoming the shortcomings of expensive price, limited sources, long manufacturing time, short preservation time, injuries to an organism caused by secondary skin grafting and the like of an existing artificial skin product. The product prepared by the preparation method provided by the invention has the advantages of extensive materials, short production cycle, long preservation time and low price, and is suitable for clinical extensive application.

Description

technical field [0001] The invention belongs to the technical field of biomaterials of tissue engineering, and in particular relates to an artificial skin prepared by ultrasound-treated collagen and polymer materials. Background technique [0002] Skin is an important tissue organ that covers and protects the body surface. Defects and abnormalities of the skin are often caused by inflammation, ulcers, trauma, burns, postoperative tumors, and congenital deformities. For this kind of tissue defect, the method of autologous skin transplantation is mostly used at present, which not only causes new trauma defects in the donor area, but also is often limited by the source of skin donor. [0003] In order to repair and replace defective skin tissue, scientists from all over the world are devoted to the research of artificial skin, but only a few countries such as the United States have mastered this technology and realized industrialization. Some artificial skin products have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/60A61L27/24A61L27/20A61L27/18A61L27/40
Inventor 刘泊志
Owner 江苏德威兰医疗器械股份有限公司
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