Preparation method and application of label-free electrochemiluminescent clenbuterol immunosensor

An immunosensor and electrochemistry technology, which is applied in the fields of chemiluminescence/bioluminescence, electrochemical variables of materials, and analysis through chemical reactions of materials, etc., can solve the problems of unfavorable luminescent system popularization and harsh reaction conditions, etc. Achieve the effects of improving detection sensitivity and reproducibility, convenient operation, and low detection limit

Active Publication Date: 2015-10-21
河南安必诺检测技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of HRP requires harsh reaction conditions, which is unfavorable for Luminol-H 2 o 2 Universal use of luminescent systems

Method used

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  • Preparation method and application of label-free electrochemiluminescent clenbuterol immunosensor
  • Preparation method and application of label-free electrochemiluminescent clenbuterol immunosensor
  • Preparation method and application of label-free electrochemiluminescent clenbuterol immunosensor

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0031] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 5 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 5 μL of AuAg NRs-CHS solution and dried at room temperature;

[0032] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 5uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 30 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0033] (3) Wash the electrode obtained in step (2) with ultra-pure water, dry it at room temperature, immerse the electrode in the EDC / NHS solution, and take it out after 1 hour;

[0034] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 5 μL of 10 μg / mL clenbuterol antibody so...

Embodiment 2

[0041] Example 2 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0042] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 8 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 8 μL of AuAg NRs-CHS solution and dried at room temperature;

[0043] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 8uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 60 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0044] (3) Same as embodiment 1;

[0045] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 8 μL of 10 μg / mL clenbuterol antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0046] (5) Wash the electrode obtained in step (4) with ...

Embodiment 3

[0051] Example 3 Preparation of label-free electrochemiluminescent clenbuterol immunosensor

[0052] (1) Use a glassy carbon electrode with a diameter of 4 mm as the working electrode, and drop-coat 10 uL TiO on the surface of the electrode 2 NPs, after drying at room temperature, drop-coated 10 μL of AuAg NRs-CHS solution and dried at room temperature;

[0053] (2) Wash the electrode obtained in step (1) with ultrapure water, dry it at room temperature to form a film, and drop-coat 10uL 0.01 mol / L H 2 PdCl 4 , and immediately irradiated with a high-pressure mercury lamp for 90 seconds to obtain TiO 2 - AuAg-Pd NDRs-CHS modified working electrode, dry at room temperature;

[0054] (3) Same as embodiment 1;

[0055] (4) Wash the electrode obtained in step (3) with ultrapure water, drop-coat 10 μL of 10 μg / mL clenbuterol antibody solution on the surface of the electrode, and store it in a refrigerator at 4 °C to dry;

[0056] (5) Wash the electrode obtained in step (4) w...

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Abstract

The invention discloses a preparation method for unmarked electrogenerated chemiluminescence clenbuterol immune sensor and belongs to the novel nano functional material and biosensor field. The dendrite nano rod-shaped precious metal alloy nano material having core-shell structure is prepared on the titanium dioxide nano-particle substrate by the photoelectric chemical synthesis method, and the unmarked electrogenerated chemiluminescence immune sensor used for detecting the clenbuterol can be prepared and the unmarked electrogenerated chemiluminescence immune sensor is low in cost, good in specificity, fast in detection speed and simple in preparation.

Description

Technical field [0001] The present invention involves the use of a fast and sensitivity to detect the preparation method and application of the non -standard electronic chemical emitting immune sensor of lean meat essence. It belongs to the technical field of new nano -function materials and biosensor. Background technique [0002] Lean essence is a type of drug called β -stimulant, not a certain drug.Such drugs have the function of promoting lean meat growth and inhibiting fat growth, so they are collectively referred to as "lean meat".According to the "Special Rectification Plan" Special Rectification of Lean Essence "(Food Safety Office [2011] No. 14), the" Lean Essence "special rectification plan of the Food Safety Committee of the State Council is 16 types of" lean meat "variety, including: Lexbamine RACTOPAMINE; ClonbutericNarrine CLORPRENALINE HYDROCHLORIDE; Mabutro Mabuteric; Cimbuteric; bromodolo brombuterol; alofolo Arformoterol Tartrate; Formoterol Fumatrate. [0003] ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/26G01N27/48G01N21/76G01N33/53
Inventor 张勇马洪敏魏琴李燕吴丹庞雪辉
Owner 河南安必诺检测技术有限公司
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