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A method for adsorption and separation of gulonic acid using ultra-high cross-linked resin

An ultra-high cross-linked resin, adsorption separation technology, applied in chemical instruments and methods, separation/purification of carboxylic acid compounds, other chemical processes, etc., can solve the problems of many unit operations, pollution, and large consumption of ion exchange resins, etc. Achieve the effects of avoiding environmental pollution, large adsorption capacity, and reducing wastewater discharge

Active Publication Date: 2017-05-24
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, the industry generally adopts the ion exchange method to separate gulong acid. This process is mature, but there are many unit operations and the regeneration of the ion exchange resin needs to consume a large amount of reagents such as acids and alkalis, causing serious pollution.

Method used

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  • A method for adsorption and separation of gulonic acid using ultra-high cross-linked resin
  • A method for adsorption and separation of gulonic acid using ultra-high cross-linked resin
  • A method for adsorption and separation of gulonic acid using ultra-high cross-linked resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1: Obtaining of gulonic acid feed liquid.

[0047] The percentage content of gulonic acid in the fermented liquid obtained by Bacillus megaterium and Ketogulonicigenium vulgare fermentation is 35% (w / w), and its fermentation process is as follows: Prepare plate culture medium: 8.0% L-sorbose, 1.0% corn steep liquor, 0.2 % urea, 0.1% KH 2 PO 4 , 0.01% MgSO 4 ·7H 2 O, 0.5% NaCl, 1.5% agar was sterilized at 121°C for 15min. The pH of the fermentation broth was adjusted to about 7.0. Add half of the nutrients initially, and then gradually add them later. Cultivate in a Bugbox anaerobic tank at 37° C. for 72 h with a stirring speed of 300 rpm. Gulong acid ~ 35g / L in the obtained fermentation broth, residual L-sorbose<1g / L.

[0048] The above-mentioned Bacillus megaterium and Ketogulonicigenium vulgare can be replaced by other bacillus to realize the fermented liquid containing gulonic acid prepared by fermentation of different strains.

[0049] The ferment...

Embodiment 2

[0050] Example 2: Preparation of ultra-high cross-linked resin HD-01.

[0051] In a 2000mL three-necked flask, add 200g of dry chloromethylated polystyrene resin, add 1500g of nitrobenzene, stir and mix well and fully swell at room temperature for 2 hours, add 12g of aluminum trichloride and 40g of divinylbenzene, and stir at room temperature After reacting for 1 hour, the temperature was raised to 95° C. at a rate of 1° C. / min to carry out the cross-linking reaction. After reacting for 8 h, 300 mL of ethanol was added to stop the reaction, and cooled to room temperature. Suction filtration, wash off the residual solvent and catalyst with ethanol, and vacuum dry to obtain the weak polar ultra-high cross-linked resin HD-01. The obtained product is in the shape of dark brown round particles, with an average particle size of 0.42-0.56mm, a water content of 43wt%, a pore size of 1.92nm, a porosity of 30-40%, and a wet density of 1.09g / cm 3 , the average specific surface area is ...

Embodiment 3

[0052] Embodiment 3: Pretreatment of ultra-high cross-linking resin HD-01.

[0053] Rinse the resin with 2BV 1mol / L HCl solution at a flow rate of 1BV / h, and then wash with water until neutral; wash the resin with 2BV 1mol / L NaOH solution at a flow rate of 1BV / h, and then wash with water until neutral; finally use 2BV Rinse the resin with ethanol at a flow rate of 2BV / h, wash away the ethanol with deionized water, and set aside.

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Abstract

The invention discloses a gulonic acid adsorption separation method utilizing hyper-crosslinked resin. According to the gulonic acid adsorption separation method utilizing the hyper-crosslinked resin, a preprocessed gulonic acid fermentation broth is subjected to the processes of absorption, impurity washing and desorption through an adsorbing column filled with the hyper-crosslinked resin, and finally a gulonic acid solution is obtained. The hyper-crosslinked resin is prepared through a Friedel-Crafts cross-linking reaction and with polystyrene-divinyl benzene as a framework, and is weak in polarity and good in hydrophilia; the average grain diameter is 0.42-0.56mm, the water content is 43wt%, the bore diameter is 1.92nm, the porosity is 30-40%, the wet density 1.09g / cm3, the average specific surface area is 1645.5m2 / g, the average pore volume is 0.732cm3 / g, and a functional group is a carbanyl group. The adsorption medium adopted by the method has the advantages of being large in adsorption capacity for the gulonic acid, good in adsorptive selectivity, mild in desorption condition and long in service cycle, enabling regeneration to be easy and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for extracting guronic acid from a raw material liquid by using an ultra-high cross-linked resin. Background technique [0002] Gulonic acid (also known as: 2-keto-L-gulnoic acid), English name α-keto-L-gulnoic-acid (abbreviated as KGA or 2-KLG), has a huge market demand in the pharmaceutical industry and food industry. Gulonic acid is an important precursor of vitamin C. Vitamin C is a vitamin necessary for human metabolism. The physiological functions of Vc in the human body mainly include: 1) participating in the oxidation-reduction reaction in the body; 2) resisting free radical damage; 3) improving the immune function of the body; 4) participating in collagen and 5) Participate in the synthesis of neurotransmitters; 6) Participate in amino acid metabolism and iron metabolism; 7) Inhibit the activation of platelets and white blood cells, etc. Therefore, Vc ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F112/08C08F8/10C08J3/24B01J20/26B01J20/30C07C59/105C07C51/47
Inventor 应汉杰柯旭吴菁岚周精卫庄伟陈勇朱晨杰
Owner NANJING TECH UNIV
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