Pharmaceutical compositions comprising glutathione and uses thereof
A technology of glutathione and composition, applied in the field of medicine and biology, can solve the problems of implant failure, poor implant repair, high initial osseointegration, etc., and achieve a significant synergistic effect
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Embodiment 1
[0024] Example 1 Effects of a pharmaceutical composition comprising GSH on the differentiation and proliferation of human osteoblast-like MG-63 cells under high glucose
[0025] MG-63 cells were cultured in MEM / EBSS medium (containing 10% fetal bovine serum (FBS) and non-essential amino acids (NEAA)) in culture flasks. The cells were passaged when the adherent confluence reached about 80-90% in the culture flask. MG-63 cells were seeded in 96-well plates at 2000 cells / well and cultured for 24 hours. After 12 hours of serum starvation, the medium was replaced with one of the following: (a) MEM / EBSS (containing NEAA, 5% FBS and 1.0 g / L glucose (physiological glucose concentration, NG)); (b) MEM / EBSS (containing NEAA, 5% FBS and 4.5 g / L glucose (high glucose concentration, HG)); supplemented medium with GSH and GSH-containing For the culture medium solution of the pharmaceutical composition, in each group, the control group was supplemented with medium a, the model group and th...
Embodiment 2
[0036] Example 2 Preparation of slow-release microspheres
[0037] Dissolve 100 mg of PLA in 1 ml of dichloromethane to make an organic phase, then add 0.1 ml of drug solution (30 mg / ml) to the organic phase, and ultrasonically (190 kW) to form colostrum; pour the colostrum into 8 ml with a concentration of 1% PVA aqueous solution, stirred at 1500rpm to form a double emulsion, then poured 7ml of PVA aqueous solution, placed on a mechanical stirrer and stirred at 600rpm for 6-8 hours, after the dichloromethane was completely volatilized, centrifuged to collect the obtained The microspheres were washed several times with distilled water, then collected by centrifugation, filtered through a Buchner leak, and freeze-dried to obtain slow-release microspheres.
[0038] Through the above-mentioned method, the present invention has prepared 5 groups of microspheres respectively, and the weight ratio of the drug composition of each group is as follows:
[0039] Weight ratio ...
Embodiment 3
[0040] Example 3 Study on the effect of slow-release microspheres on the osseointegration level around implants in type 2 diabetic rats
[0041] 8-week-old GK rats were adaptively fed for one week, and continuously fed with high-fat and high-sugar diet for 4 weeks. The rats with blood glucose 18.0 mmol / L were excluded by monitoring the blood glucose of the rats, thereby reducing type 2 DM. The mixed bias of rat blood glucose values in this experiment ensures the smooth progress of the experiment.
[0042] The rats were successfully anesthetized with 2% sodium pentobarbital. After anesthesia, the skin of the right lower limb of the rat was prepared. A longitudinal incision of about 2 cm in length was made along the tibia at the femoral head of the right lower limb of the rat, and the muscle tissue was bluntly separated. Tendon and periosteum were exposed, and the tibial epiphysis was exposed. Circular dental drills with diameters of 2.2 mm and 2.8 mm were used to prepare imp...
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Abstract
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