Complex of helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid as well as preparation method and application of complex

A technology of single-walled carbon nanotubes and polysaccharide derivatives, which is used in pharmaceutical combinations, medical preparations with inactive ingredients, and medical preparations containing active ingredients, etc., can solve the problem that the water dispersion stability of single-walled carbon nanotubes needs to be improved. , Destruction of the chemical structure of SWCNTs, the dispersion stability needs to be improved, etc., to achieve obvious photothermal conversion effect, improve biocompatibility, and easy to implement.

Active Publication Date: 2015-04-29
GUANGZHOU REFORGENE MEDICINE CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, such research is still very limited at present, and the main problems include: (1) The modification of SWCNTs by cationic polymers requires multi-step chemical reactions, which is not only cumbersome to operate, but also destroys the chemical structure of SWCNTs itself, affecting its performance; (2) The cationic polymer used is usually polyethyleneimine (PEI), etc., with poor biocompatibility; (3) The water dispersion stability of this type of functionalized single-walled carbon nanotubes needs to be improved
The preparation method is simple and easy to implement, and the obtained functionalized single-walled carbon nanotubes have good biocompatibility, but their dispersion stability in aqueous solution needs to be improved, and the cationic modification enables them to bind negatively charged organisms such as nucleic acids. Active molecules, which have not been reported in the prior art

Method used

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  • Complex of helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid as well as preparation method and application of complex
  • Complex of helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid as well as preparation method and application of complex
  • Complex of helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid as well as preparation method and application of complex

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Weigh 1g of potato amylose and dissolve it in 50mL of dimethyl sulfoxide, under the protection of nitrogen, add 1g of CDI, stir and react at 30°C for 2h, then add dropwise 10 times the molar amount of CDI to the system ethylenediamine, continue to stir and react for 22h, then transfer to a dialysis bag with a molecular weight cut-off of 14000 for dialysis with water for 2 days, and obtain the amylose derivative (EA-Amy-I) containing the primary amino group after freeze-drying;

[0039] (2) EA-Amy-I was dissolved in water to prepare a solution of 2 mg / mL, adding 0.5 times the single-walled carbon nanotubes (length 1-3 μm, diameter 1-2nm) of EA-Amy-I quality, and then Use 65kHz ultrasonic waves in the ice-water bath for treatment, and the specific cycle is 30 minutes every 5 minutes, and a total of 3 cycles of treatment are performed. The obtained mixed system is centrifuged at 1000 rpm for 3 minutes, and the obtained dispersion is filtered with a 220 nm microporous memb...

Embodiment 2

[0050] (1) Weigh 1g of potato amylose and dissolve it in 50mL of dimethyl sulfoxide. Under the protection of nitrogen, add 3g of CDI, stir and react at 30°C for 2h, and then dropwise add 10 times the molar amount of CDI to the system ethylenediamine, continue to stir and react for 22h, and transfer to a dialysis bag with a molecular weight cut-off of 14000 for dialysis with water for 2 days, and obtain an amylose derivative (EA-Amy-II) containing primary amino groups after freeze-drying;

[0051] (2) EA-Amy-II was dissolved in water to prepare a solution of 2mg / mL, adding 0.5 times the quality of EA-Amy-II single-walled carbon nanotubes (length 1-3μm, diameter 1-2nm), and then Use 65kHz ultrasonic waves in the ice-water bath for treatment, and the specific cycle is 30 minutes every 5 minutes, and a total of 3 cycles of treatment are performed. The obtained mixed system is centrifuged at 1000 rpm for 3 minutes, and the obtained dispersion is filtered with a 220 nm microporous me...

Embodiment 3

[0062] (1) Weigh 1g of corn amylose and dissolve it in 40mL of dimethyl sulfoxide. Under the protection of nitrogen, add 0.1g of CDI, stir and react at 25°C for 1h, and then dropwise add 2 times the molar amount of CDI to the system. amount of ethylenediamine, after continuing to stir and react for 23h, transfer it to a dialysis bag with a molecular weight cut-off of 1000 for dialysis with water for 7 days, and obtain amylose derivatives containing primary amino groups after freeze-drying;

[0063] (2) the amylose derivative of step (1) is dissolved in water and is formulated into a solution of 5 mg / mL, and single-walled carbon nanotubes (length 0.5-2 μm, diameter 3- 5nm), and then use 40kHz ultrasonic waves in an ice-water bath to process, the specific cycle is ultrasonic 20min every 1min, a total of 4 cycles of processing, the resulting mixed system is centrifuged at 3000rpm for 1min, and the resulting dispersion is separated by a 220nm micro filter through a pore filter, re...

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Abstract

The invention belongs to the technical field of biomedical materials, and discloses a complex of a helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid as well as a preparation method and application of the complex. The preparation method of the complex comprises the following steps: enabling helical chain polysaccharide amylase to react with N, N'-carbonyldimidazole and ethylenediamine in dimethyl sulfoxide to obtain a water-soluble amylase derivative containing primary amino groups, then adding a single-walled carbon nanotube into an aqueous solution, and performing ultrasonic treatment, centrifugation, filtration and freeze drying to obtain an amylase derivative-single-walled carbon nanotube inclusion compound; and then, scattering the inclusion compound into double distilled water, adding nucleic acid, fully vibrating, and then performing incubation to obtain a complex of the helical chain polysaccharide derivative co-loaded single-walled carbon nanotube and nucleic acid. The complex disclosed by the invention combines photo-thermal conversion effects of the inclusion compound and special functions of nucleic acid, and can be used for preparing medicines for photo-thermal and gene combined treatment.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a composite of helical chain polysaccharide derivatives co-supporting single-walled carbon nanotubes and nucleic acids and its preparation and application. Background technique [0002] In recent years, single-walled carbon nanotubes (SWCNTs) modified by water-soluble cationic polymer segments have shown good application prospects in the fields of photothermal-gene combined therapy (Wang L., et al. Biomaterials, 2013 , 34, 262-274.). On the one hand, SWCNTs, as a new type of carbon-structured one-dimensional nanomaterial with highly delocalized π-electron conjugated system, has the characteristics of responding to near-infrared light and generating photothermal conversion, and can be used for photothermal therapy of tumors (Moon H.K. , et al.ACS nano,2009,3,3707-3713; Liu X.W.,et al.Biomaterials,2011,32,144-151.); On the other hand, the water-soluble cat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/48A61K41/00A61K48/00A61P35/00A61K47/52A61K47/61
Inventor 张黎明麦开锦李小军何杨华
Owner GUANGZHOU REFORGENE MEDICINE CO LTD
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