Microfluidic chip preparation method for culturing and detecting lung cancer cells

Abstract

The invention discloses a method for preparing a micro-fluidic chip for culturing and detecting lung cancer cells. The method comprises the following steps: preparing a mask; preparing a hydrogel material; preparing a cell culture layer; arranging the mask of the cell culture layer on a glass substrate, and injecting the hydrogel material under shading conditions, so that the hydrogel material is cured; preparing a micro-channel layer, namely arranging the mask of the micro-channel layer on the prepared cell culture layer, and injecting the hydrogel material under the shading conditions, so that the hydrogel material is cured; and eluting and preparing a sealing cover layer, namely eluting the prepared micro-channel layer, and sealing by using PDMS. According to the micro-fluidic chip prepared by the method disclosed by the invention, multiple groups of cell samples can be simultaneously cultured, a three-dimensional growth environment is provided for the cells, the human lung tissue structure is completely simulated, a growth environment close to the human body is provided for the cells, and the metabolite is very close to the metabolite of human cells, so that the detection data is closer to the human real conditions, and early diagnosis of lung cancer is promoted.

Description

technical field [0001] The invention relates to a method for preparing a microfluidic chip, in particular to a method for preparing a microfluidic chip for cultivating and detecting lung cancer cells. Background technique [0002] Lung cancer, also known as bronchial cancer, occurs in the epithelium of the bronchial mucosa. It is one of the malignant tumors with the fastest-growing morbidity and mortality and the greatest threat to the health and life of the population. Because the symptoms of lung cancer are not obvious in the early stage, it is often discovered at an advanced stage. Therefore, early diagnosis and early treatment of lung cancer are the most effective ways to prevent and treat lung cancer and reduce the mortality rate. At present, clinically, the common diagnostic methods for lung cancer are as follows: X-ray detection, computerized tomography (CT), magnetic resonance tomography (MRI), sputum exfoliated cell examination, fiber bronchial examination, percutan...

AI Technical Summary

Problems solved by technology

However, microfluidic chip technology is still in its infancy, and its related technologies and methods for lung cancer cell culture and detection have not been reported yet.

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