Method for adding lucid ganoderma to assist fermentation of straw silage feed
A technology for assisting fermentation and silage, applied in the field of silage, can solve the problems of unsatisfactory effect of silage production cycle and quality improvement, poor quality of silage, low L-lactic acid content, etc., to improve animal immunity, The effect of shortening the fermentation period and improving the quality of silage
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0035] The method for adding Ganoderma lucidum to assist fermentation of wheat straw silage comprises the following steps:
[0036] (1) Activation and expanded culture of the strain:
[0037] Inoculate Ganoderma lucidum on fungal solid medium, restore the strains in a plate, that is, activate once, and culture at 30°C for 3 days; take the activated bacteria on the plate and inoculate in 100ml fungal liquid medium for second activation, the activation condition is 26 ℃, rotation number 120rpm, cultivated for 5 days to obtain a uniform bacterial suspension; inoculate the uniform bacterial suspension in a liquid fermenter at a ratio of 0.5% of the volume ratio of the uniform bacterial suspension to the culture solution in the fermenter, and keep Ventilation, stirring speed 200-600rpm (rotation speed related to dissolved oxygen), controlled dissolved oxygen 30%, fermented for 6 days to obtain a fermentation broth containing uniformly suspended mycelia with a pH of 4.5±0.5;
[003...
Embodiment 2
[0043] Processing and quality control of Ganoderma lucidum assisted fermentation rice straw silage
[0044] (1) Activation and expanded cultivation of bacterial strains: inoculate Ganoderma lucidum strains on fungal solid medium (per liter contains 35g of glucose, 5g of peptone, 2.5g of yeast extract, 1g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, vitamin B 10.05g, agar powder 150g), carry out the recovery of bacterial classification (namely primary activation) in the plate, culture 3 days at 28 ℃. Take the activated bacteria on the plate and inoculate them into 100ml fungal liquid medium (each liter contains 35g of glucose, 5g of peptone, 2.5g of yeast extract, 1g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, vitamin B 1 0.05 g) was activated for the second time, and the activation conditions were 28° C., 150 rpm for 3 days, and a uniform bacterial suspension was obtained. Inoculate the uniform bacterial suspension in the liquid fermenter at...
Embodiment 3
[0048] The method for adding Ganoderma lucidum to assist fermentation of corn stalk silage comprises the following steps:
[0049] (1) Activation and expanded culture of the strain:
[0050] Inoculate Ganoderma lucidum on fungal solid medium, restore the strains in a plate, that is, activate once, and culture at 26°C for 5 days; take the activated bacteria on the plate and inoculate in 100ml fungal liquid medium for the second activation, the activation condition is 30 ℃, rotation number 180rpm, cultivated for 3 days to obtain a uniform bacterial suspension; inoculate the uniform bacterial suspension in a liquid fermenter at a ratio of 2% of the volume ratio of the uniform bacterial suspension to the culture solution in the fermenter, and keep Ventilation, stirring speed 200-600rpm (rotation speed related to dissolved oxygen), controlled dissolved oxygen 20%, fermented for 7 days to obtain a fermented liquid containing evenly suspended mycelium with a pH of 4.5±0.5;
[0051] ...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com