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Method for adding lucid ganoderma to assist fermentation of straw silage feed

A technology for assisting fermentation and silage, applied in the field of silage, can solve the problems of unsatisfactory effect of silage production cycle and quality improvement, poor quality of silage, low L-lactic acid content, etc., to improve animal immunity, The effect of shortening the fermentation period and improving the quality of silage

Inactive Publication Date: 2015-05-06
NANTONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Natural fermentation is mostly used in the production of silage, but the straw itself has very few lactic acid bacteria and its own nutritional content is limited, which cannot guarantee that lactic acid bacteria will become the dominant flora during the fermentation process, and the most beneficial component of silage is L-lactic acid. Content is not high , so the quality of the produced silage is not good
At home and abroad, the research on special additives for silage has been relatively active, but the addition is mostly limited to aerobic bacteria and facultative lactic acid bacteria to improve the level of lactic acid fermentation, and the effect on the production cycle and quality improvement of silage is not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The method for adding Ganoderma lucidum to assist fermentation of wheat straw silage comprises the following steps:

[0036] (1) Activation and expanded culture of the strain:

[0037] Inoculate Ganoderma lucidum on fungal solid medium, restore the strains in a plate, that is, activate once, and culture at 30°C for 3 days; take the activated bacteria on the plate and inoculate in 100ml fungal liquid medium for second activation, the activation condition is 26 ℃, rotation number 120rpm, cultivated for 5 days to obtain a uniform bacterial suspension; inoculate the uniform bacterial suspension in a liquid fermenter at a ratio of 0.5% of the volume ratio of the uniform bacterial suspension to the culture solution in the fermenter, and keep Ventilation, stirring speed 200-600rpm (rotation speed related to dissolved oxygen), controlled dissolved oxygen 30%, fermented for 6 days to obtain a fermentation broth containing uniformly suspended mycelia with a pH of 4.5±0.5;

[003...

Embodiment 2

[0043] Processing and quality control of Ganoderma lucidum assisted fermentation rice straw silage

[0044] (1) Activation and expanded cultivation of bacterial strains: inoculate Ganoderma lucidum strains on fungal solid medium (per liter contains 35g of glucose, 5g of peptone, 2.5g of yeast extract, 1g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, vitamin B 10.05g, agar powder 150g), carry out the recovery of bacterial classification (namely primary activation) in the plate, culture 3 days at 28 ℃. Take the activated bacteria on the plate and inoculate them into 100ml fungal liquid medium (each liter contains 35g of glucose, 5g of peptone, 2.5g of yeast extract, 1g of potassium dihydrogen phosphate, 7.5g of magnesium sulfate, vitamin B 1 0.05 g) was activated for the second time, and the activation conditions were 28° C., 150 rpm for 3 days, and a uniform bacterial suspension was obtained. Inoculate the uniform bacterial suspension in the liquid fermenter at...

Embodiment 3

[0048] The method for adding Ganoderma lucidum to assist fermentation of corn stalk silage comprises the following steps:

[0049] (1) Activation and expanded culture of the strain:

[0050] Inoculate Ganoderma lucidum on fungal solid medium, restore the strains in a plate, that is, activate once, and culture at 26°C for 5 days; take the activated bacteria on the plate and inoculate in 100ml fungal liquid medium for the second activation, the activation condition is 30 ℃, rotation number 180rpm, cultivated for 3 days to obtain a uniform bacterial suspension; inoculate the uniform bacterial suspension in a liquid fermenter at a ratio of 2% of the volume ratio of the uniform bacterial suspension to the culture solution in the fermenter, and keep Ventilation, stirring speed 200-600rpm (rotation speed related to dissolved oxygen), controlled dissolved oxygen 20%, fermented for 7 days to obtain a fermented liquid containing evenly suspended mycelium with a pH of 4.5±0.5;

[0051] ...

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PUM

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Abstract

The invention discloses a method for adding lucid ganoderma to assist fermentation of a straw silage feed. The method comprises the following steps: (1) performing activation and enlarge cultivation of a bacterial strain: inoculating the lucid ganoderma to a fungus solid culture medium, restoring a strain in a flat plate, namely activating for the first time; inoculating the strain which is activated in the flat plate to a fungus liquid culture medium to perform activating for rhe second time to obtain a uniform bacterial suspension; inoculating the bacterial solution which is activated for the second time in a liquid fermentation tank, keeping ventilation, and fermenting to obtain fermentation liquor comprising uniformly suspended hyphae; (2) inoculating and fermenting the straw silage feed: inoculating the fermentation liquor comprising uniformly suspended hyphae into a fermentation substrate of straws, fermenting in a sealing manner, placing for 15-60 days at a normal temperature to obtain the straw silage feed. The method is simple to operate and not high in selectivity to the fermentation substrate; in addition to meeting needs of acidity, straw storage, feeding palatability and the like, the straw silage feed obtained by fermenting further can be used for greatly shortening the fermentation period, reducing the fermentation cost and increasing the nutrients of the straw silage feed.

Description

technical field [0001] The invention relates to the field of silage, in particular to a method for adding Ganoderma lucidum to assist fermentation of straw silage. Background technique [0002] The world's annual output of straw is about 2-3 billion tons, and China's annual output of various crop straws is about 700 million tons. However, due to various reasons, more than 1 / 3 of the straw is discarded or directly burned, causing serious environmental pollution. It also caused a great waste of resources. [0003] Plant straw contains rich nutrients and is suitable for fertilizer or feed utilization. Although straw can be directly fed to livestock, the straw itself has poor taste and low nutritional value, or livestock do not like to eat it due to aging, dryness and other reasons. In the natural state, the crude protein content of crop straw is low, generally 3-5%, and the quality is not good; the crude fiber content is too high, the digestibility is low, and the palatabilit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/00A23K1/14
CPCY02P60/87
Inventor 卢晓凤徐磊全晓天常燕谢焕松张新中
Owner NANTONG UNIVERSITY
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