The invention discloses a novel animal padding and a preparation method thereof, and relates to the technical field of animal tools. The padding comprises the following raw materials in parts by weight of 500-650 parts of attapulgite, 1-3 parts of starch, 1-3 parts of a binder, 0.5-2 parts of a preservative, 0.5-2 parts of an inorganic deodorant and 150-200 parts of water. The padding product is an oblate sphere, the long diameter is 0.3-0.5 mm, the short diameter is 0.3 mm, the natural water content is smaller than or equal to 5%, the color is uniform and consistent, no obvious color difference exists, no pungent smell exists, no mildewing smell exists, and the padding product has certain water absorption capacity, heat preservation performance, bacteriostasis and deodorization functions, and is high in hardness and non-toxic. The padding disclosed by the invention is a non-agglomerated padding; and a mouse feeding experiment proves that compared with a traditional padding, the padding disclosed by the invention has a certain inhibition effect on microorganisms in excrement, the replacement frequency is low, and the use cost is remarkably reduced. The required raw material types are few, the attapulgite is used as the main component, the preparation process is simple, the cost is low, and the popularization and use are facilitated.