The invention relates to a health care wine and a preparation method thereof. The wine contains 20-60 parts of cistanche, 20-40 parts of herba epimedii, 10-30 parts of mulberry, 10-30 parts of Chinese wolfberry, 10-30 parts of cornus officinalis, 10-30 parts of jujube, 1-2 parts of cervus elaphus linnaeus, and 45-65 parts of rock sugar. Soaking, filter, blending, wine storage, filling, capping and other processes are carried out to prepare the health care wine. The wine provided by the invention well solves the problems of short preservation period in traditional health care products, by preparing the materials into wine, the drug efficacy is improved, several traditional Chinese medicines in a reasonable ratio are made into the health care wine with the multiple health care effects of benefiting qi and tonifying deficiency, moistening five internal organs, harmonizing blood vessels, benefiting essence qi, supplementing essence, strengthening muscles and bones, invigorating spleen and stomach, nourishing yin and reinforcing yang, etc. The wine base is soaked, water is added to allocate the alcohol content, and rock sugar is added to blend the sugar degree, thus obtaining the product. Sensory tests show that the product is clear and transparent, has good taste, and has extensive adaptability in treatment of chronic fatigue syndromes and the health care field.