Recombinant lactobacillus plantarum capable of secreting colicins V and preparation method thereof

A technology of colibactin and Lactobacillus plantarum, applied in the field of microbial molecular biology, can solve problems such as animal intestinal flora imbalance, threats to the breeding industry and feed industry, and bacterial resistance to superinfection
CN104593404AInactive Publication Date: 2015-05-06CHINA AGRI UNIV
1 Cites 1 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHINA AGRI UNIV
Publication Date
2015-05-06
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention relates to a recombinant lactobacillus plantarum capable of secreting colicins V and a preparation method thereof, belonging to the field of microbe molecule biology. The recombinant lactobacillus plantarum disclosed by the invention carries a free expression vector containing a pediocin PA-1 leading peptide-mature colicin V fused gene. The recombinant lactobacillus plantarum is prepared through connecting a pediocin PA-1 leading peptide-mature colicin V peptide fused gene to an expression vector, transferring a connecting product to lactococcus lactis by using an electrotransformation method, extracting a recombinant plasmid, and transferring the recombinant plasmid to lactobacillus plantarum BM-1 through electrotransformation. The recombinant lactobacillus plantarum disclosed by the invention can simultaneously secrete pediocin PA-1 and colicin V, and has a good inhibitory effect on colicins and listeria monocytogenes pathogenic bacteria; after being eaten by animals, the recombinant lactobacillus plantarum does not produce toxic and side effects and residues, so that the recombinant lactobacillus plantarum has no threat to the environment and food security, therefore, the recombinant lactobacillus plantarum has a good application prospect in the fields of feed industry and food industry.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the field of microbial molecular biology, in particular to a recombinant Lactobacillus plantarum secreting colicin V and a preparation method thereof. Background technique

[0002] In the past 50 years, antibiotics, as the most commonly used disease prevention and growth-promoting feed additives, have brought benefits to the aquaculture industry, but also brought fatal disadvantages: animal intestinal flora imbalance, superinfection and bacterial resistance. Sexual and other adverse reactions have gradually become prominent, posing a serious threat to the aquaculture and feed industries. Therefore, the development of non-toxic, non-polluting and non-residue antibiotic alternatives has become a top priority for scientific researchers. Since the 1980s, microecological preparations based on lactic acid bacteria have begun to attract attention, and their functions of promoting the growth of intestinal beneficial bacteria, inhibiti...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More