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Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe

A ratiometric fluorescent probe and quantum dot technology, applied in the field of ratiometric fluorescent probes, achieves the effects of improving photostability and biocompatibility, improving anti-interference ability and low price

Inactive Publication Date: 2015-05-06
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, quantum dots as fluorophores have been successfully applied to the ratiometric fluorescence detection of metal ions such as copper ions and mercury ions, but the use of quantum dot fluorescent probes to achieve the detection of zinc ions through ratiometric fluorescence has not been reported.

Method used

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  • Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe
  • Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe
  • Quantum dot rate fluorescence probe for zinc ion detection and detection method of quantum dot rate fluorescence probe

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Embodiment 1

[0023] Quantum dot ratio fluorescent probe aqueous solution for zinc ion detection according to the present invention is formed of quantum dots / An aqueous solution of silica fluorescent nanoparticles, wherein the surface of quantum dots / silica nanoparticles is modified with positively charged amino groups, and the diameters are about 20nm. The porphyrin molecules are negatively charged sulfur-based porphyrin molecules with a concentration of 3×10 -6 mol / L, combined on the surface of quantum dots / silicon dioxide particles through electrostatic interaction to form a quantum dots / silicon dioxide-porphyrin solution.

[0024] Different concentrations of zinc ions were added to the quantum dot / silica-porphyrin solution. After the porphyrin molecules were completely complexed with the zinc ions, the green fluorescent quantum dots and yellow-emitting quantum dots in the probe were measured under different zinc ion concentrations. Fluorescence intensity of the fluorescent quantum dot...

Embodiment 2

[0027] The quantum dot ratio fluorescent probe for zinc ion detection in this embodiment is made of quantum dot / silica fluorescent nanoparticles and porphyrin molecules, and the concentration of porphyrin is 3×10 -6 mol / L, the concentrations of zinc ions and interfering ions in the solution to be tested are both 1.2×10 -5 mol / L, the fluorescence intensities of green fluorescent quantum dots and yellow fluorescent quantum dots were measured under different ions, and their ratios were calculated. After interfering ions are added to the quantum dot / silica-porphyrin solution, the ratio of the fluorescence intensity of the green fluorescent quantum dots and the yellow fluorescent quantum dots does not change, as Figure 4 shown. Only when zinc ions exist, the ratio of fluorescence intensity of green fluorescent quantum dots and yellow fluorescent quantum dots can be significantly enhanced. It shows that this probe has good selectivity to zinc ions.

Embodiment 3

[0029] The present embodiment detects zinc ions in mineral water and tap water according to the following steps: (1) After adding the zinc ion concentration unknown mineral water and tap water sample solution respectively in the quantum dot / silicon dioxide-porphyrin solution, the porphyrin molecules It is fully complexed with zinc ions, and the fluorescence intensity of the green fluorescent quantum dots and yellow fluorescent quantum dots is measured and their ratios are calculated. (2) Compare the measured fluorescence intensity ratio with the standard curve drawn in Example 1, and calculate the zinc ion concentration in the unknown mineral water and tap water samples.

[0030] In this embodiment, the concentration of zinc ions detected by the designed ratiometric fluorescent probe is consistent with the concentration detected by atomic absorption spectrometry, indicating that the present invention is applicable to the detection of cadmium ions in environment and drinking wat...

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Abstract

The invention discloses a quantum dot rate fluorescence probe for zinc ion detection and a detection method of the quantum dot rate fluorescence probe. The quantum dot rate fluorescence probe for zinc ion detection is prepared from CdSe / ZnS quantum dots for emitting green fluorescence, and CdSe / CdZnS / ZnS quantum dots for emitting yellow fluorescence and porphyrin molecules in an electrostatic assembly manner; the molar concentration ratio of the two quantum dots to porphyrin is 1 to 10; the outer layers of the two quantum dots are wrapped by positively charged silicon dioxide shell layers; and the porphyrin molecules are negatively charged sulfur-based porphyrin molecules. By virtue of a rate fluorescence detection method, the quantum dot rate fluorescence probe adopts a simple process, is low in price, good in repeatability, suitable for the environment, drinking water and body liquid, and has a good application prospect.

Description

technical field [0001] The invention relates to a ratio fluorescence probe, in particular to a quantum dot ratio fluorescence probe for detecting zinc ions and a method for detecting zinc ions in water by using the ratio fluorescence probe. Background technique [0002] The content of zinc ions in the human body is second only to iron ions as excessive metal ions. It is widely distributed in human cells and body fluids. In many life activities such as immune regulation, growth and development, tissue repair, signal transmission, and gene transcription, Zinc ions all play a vital role. The disorder of zinc ion metabolism in the human body is closely related to the occurrence of Alzheimer's disease, diabetes, epilepsy and other diseases. The upper limit of zinc ion content stipulated in my country's drinking water hygiene standard (GB5749-2006) is 1.5×10 -5 mol / L. Therefore, it is of great significance to realize the highly sensitive detection of zinc ions in drinking water...

Claims

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Application Information

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IPC IPC(8): G01N21/64
Inventor 孙清江巫亮郭庆生刘玉乾
Owner SOUTHEAST UNIV
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