Biological protein preparation for preventing and controlling citrus huanglongbing and preparation method for biological protein preparation

Abstract

The invention discloses a biological protein preparation for preventing and controlling citrus huanglongbing and a preparation method for the biological protein preparation, and belongs to the technical field of biopesticides. According to the adopted technical scheme, the biological protein preparation is formed by compounding NAC protein and a streptomyces hygroscopicus metabolite. The preparation method comprises the following steps: (1) selecting a streptomyces hygroscopicus strain, performing activation, culture expansion and fermentor culture on the streptomyces hygroscopicus strain, and stopping fermentation when the concentration of a thallus reaches 60-80 percent to obtain streptomyces hygroscopicus; (2) performing cell disruption on the streptomyces hygroscopicus obtained in the step (1), and concentrating to remove 70-90 percent of the volume of water to obtain a condensed fermentation broth; (3) in parts by mass, weighing 20-40 parts of the condensed fermentation broth, 1-1.5 parts of NAC protein, 20-30 parts of a carrier and 5-8 parts of an additive, uniformly mixing, drying and grinding. The preparation is friendly in environment, simple in preparation process, non-toxic and high in efficiency, has a good prevention and control effect on the citrus huanglongbing, enables leaves of infected plants to become green from yellow, and has a certain recovery effect on the infected plants.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and in particular relates to a biological protein preparation for preventing and controlling citrus huanglongbing and a preparation method thereof. Background technique [0002] Citrus huanglongbing is caused by a Gram-negative bacterium limited to the phloem. This pathogen cannot be isolated and artificially cultivated in the world. It is difficult to test. Various Rutaceae plants such as Lixiang are devastating diseases in the world's citrus production. At present, the disease is mainly distributed in nearly 50 countries and regions in Asia, Africa, Oceania, South America and North America. my country is a big citrus producing country. In 2006, the planting area was 1.882 million hectares, accounting for 24% of the world's citrus planting area, ranking first in the world; the total output was 18.425 million tons, accounting for 16% of the world's total output, ranking second in t...

AI Technical Summary

Problems solved by technology

People have reached a consensus that only by preventing and controlling Huanglongbing disease, removing the biggest obstacle in the development of the citrus industry, and ensuring safe production, can the benefits be improved so that the quantity and quality of the fruit can meet the needs of consumers. Focusing on prevention, most of the infected plants adopt the method of controlling psyllids or eradicating plants or spraying organic pesticides, which is labor-intensive, affects income and has a great impact on fruits, soil, the environment and human health. Therefore, research It is very important for the citrus industry to prepare environmentally friendly, less toxic or non-toxic agents for effective control of Huanglongbing

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