Application of a kind of seaweed sesquiterpene compound

A technology for sesquiterpenoids and compounds is applied in the application field of seaweed sesquiterpenoids, which can solve the problems of bacterial drug resistance, ecological environment destruction and the like, and achieve significant bacteriostatic activity, wide application prospects, and easy preparation. Effect

Active Publication Date: 2017-08-22
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, antibiotics are mainly used to treat these two kinds of bacteria, but antibiotics are easy to make bacteria resistant, and long-term use of antibiotics will cause damage to the ecological environment

Method used

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  • Application of a kind of seaweed sesquiterpene compound
  • Application of a kind of seaweed sesquiterpene compound
  • Application of a kind of seaweed sesquiterpene compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The preparation method of embodiment 1 seaweed sesquiterpenoids (1)

[0019] (1) Collect fresh Laurencia okamurai, wash with seawater, remove sand and algae attached to the surface, dry in the shade, and then pulverize. Soak and extract with methanol-dichloromethane (volume ratio 3:1), combine the extracts and concentrate to obtain a crude extract; extract the crude extract three times with ethyl acetate and concentrate under reduced pressure at a temperature of 40°C to obtain the crude extract after extraction. Extract 16.0g.

[0020] (2) The crude extract was subjected to 200-300 mesh silica gel column chromatography, and petroleum ether-ethyl acetate was used at 100:0, 50:1, 20:1, 10:1, 5:1, 2:1 to 0 :100 gradient for elution, collect the eluate respectively, and then detect with thin layer chromatography (TLC) (vanillin-sulfuric acid color development), judge according to the Rf value, merge the same or similar parts, and obtain 14 groups points (1-14). Fraction ...

Embodiment 2

[0024] The preparation method of embodiment 2 seaweed sesquiterpenoids (2)

[0025] (1) Collect fresh Laurencia okamurai, wash with seawater, remove sand and algae attached to the surface, dry in the shade, and then pulverize.

[0026] Soak and extract with 95% ethanol, combine the extracts and concentrate to obtain a crude extract; extract the crude extract three times with ethyl acetate and concentrate under reduced pressure at a temperature of 40°C to obtain 11.8 g of the extracted crude extract.

[0027] (2) The crude extract was subjected to 200-300 mesh silica gel column chromatography, using petroleum ether-ethyl acetate with a gradient of 50:1, 20:1, 10:1, 5:1, 2:1, 1:1 Carry out elution, collect eluate separately, detect (vanillin-sulfuric acid color development) with thin-layer chromatography (TLC) again, judge according to Rf value, merge identical or similar part, obtain 10 components (1~ 10). Component 2, that is, the component eluted with petroleum ether-ethyl ...

Embodiment 3

[0030] Embodiment 3 antibacterial activity experiment

[0031] The bacterial culture medium used in the experiment was NB nutrient broth medium, and the bacterial suspension was soaked with a sterile cotton swab, and spread evenly on the M-H medium. The seven strains used were Streptococcus agalactiae, Vibrio anguillarum, Aeromonas hydrophila, Vibrio taburi, Streptococcus iniae, Edwardsiella and HN016.

[0032] The test samples of the compound prepared in Example 1 were dissolved in methanol at concentrations of 5.0, 2.5, 1.5, 1 and 0.5 mg / ml, respectively. Take 10 microliters of sample solution and add it to a sterile filter paper sheet with a diameter of 5 mm (50, 25, 15, 10 and 5 micrograms per sheet), test the antibacterial effect under different gradients; and add the same volume of methanol and The dried filter paper was used as a negative control, and erythromycin, amikacin, chloramphenicol, cotrimoxazole and gentamicin were used as antibacterial positive controls, eac...

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Abstract

The invention relates to the field of chemical and biological medicines, and particularly relates to applications of an algae sesquiterpenoid compound, especially applications in preparation of a bacteriostatic agent. The structural formula of the compound is shown as (I), and the compound is extracted from Laurencia okamurai. Experimental results show that the compound has strong bacteriostatic activity, especially the specific inhibition effect on streptococcus agalactiae and vibrio anguillarum. The structural formula is shown in the description.

Description

technical field [0001] The invention relates to the field of antibacterial agents, in particular to the application of seaweed sesquiterpenoids. Background technique [0002] Laurencia okamurai (Laurencia okamurai) belongs to the class of Rhodophyta, Rhodophyceae, Cephalophyta, Pinustaceae, and the genus of Eucalyptus seaweed in taxonomic status. It is mainly distributed in China, Japan, and the Korean Peninsula and other coastal areas in the western Pacific Ocean. areas, generally growing on intertidal rocks. Sesquiterpenoids are the most abundant class of secondary metabolites in S. Okamura, and they have diverse biological activities. [0003] Streptococcus agalactiae (S. agalactiae) is the pathogen of mastitis. It often exists in the skin, teats and udders of dairy cows, and is transmitted by the milking staff's hands or milking machinery and flies. This streptococcus does not produce obvious immunity after causing mastitis, and there is currently no reliable multivale...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/05A61P31/04C07C37/70C07C39/17
Inventor 贾睿杨欣欣蔡春尔何培民邱军强王维维谈吉张建伟
Owner SHANGHAI OCEAN UNIV
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