Preparation method of feeding lactic acid bacteria micro pills

A lactic acid bacteria and feeding technology, which is applied in the field of preparation of lactic acid bacteria pellets for feeding, can solve the problems of insignificant improvement of lactic acid bacteria tolerance, inability of lactic acid bacteria to resist damage, low survival rate of lactic acid bacteria, etc., and achieves easy large-scale application, The effect of good protection and large production capacity

Active Publication Date: 2015-05-27
SICHUAN GAOFUJI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in practical applications, this method uses a single high-salt or single high-temperature or low-temperature stress treatment on Lactobacillus plantarum for a short time in the late logarithmic phase, and the improvement of tolerance to lactic acid bacteria is not obvious, and lactic acid bacteria cannot resist post-processing. The various destructive effects of lactic acid bacteria make the survival rate of lactic acid bacteria lower

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  • Preparation method of feeding lactic acid bacteria micro pills
  • Preparation method of feeding lactic acid bacteria micro pills
  • Preparation method of feeding lactic acid bacteria micro pills

Examples

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Effect test

Embodiment 1

[0029] A preparation method of lactic acid bacteria pellets for feeding is prepared through the following steps;

[0030] (1) Preparation of lactic acid bacteria sludge: when Lactobacillus plantarum is cultured in the MRS culture medium to the mid-logarithmic growth phase, about 10 to 16 hours, a certain amount of sodium chloride is added to the respective culture medium to chlorinate the culture medium The final concentration of sodium is controlled at 0.4mol / L; at this concentration, continue to cultivate Lactobacillus plantarum until the early stage of the stable growth period, about 6-10 hours, centrifuge at a centrifugal acceleration of 4600g for 10 minutes, and wash the bacteria sludge with 0.85% normal saline Twice, collect the sludge, then resuspend the sludge in fresh MRS medium, slowly raise the temperature to 45°C, and keep it at this temperature for 30 minutes, then quickly cool it to 0-4°C, and set aside;

[0031] (2) Weigh 200 parts of prefabricated gelatinized s...

Embodiment 2

[0034] A preparation method of lactic acid bacteria pellets for feeding is prepared through the following steps;

[0035] (1) Preparation of lactic acid bacteria sludge: when Lactobacillus casei is cultured in the MRS culture medium to the mid-logarithmic growth phase (about 10-16 hours), a certain amount of sodium chloride is added to the respective culture medium to make the chlorine in the culture medium The final concentration of sodium chloride was controlled at 0.5mol / L; at this concentration, the lactic acid bacteria were continued to be cultured until the early stage of the stable growth period (about 6-10h), centrifuged at a centrifugal acceleration of 4600g for 10min, and washed with 0.85% normal saline. Sludge twice, collect the sludge, then resuspend the sludge in fresh MRS medium, slowly raise the temperature to 47°C, and keep it at this temperature for 30min, then cool it down to 0-4°C quickly, and set aside;

[0036] (2) Weigh 220 parts of microcrystalline cellu...

Embodiment 3

[0039] A preparation method of lactic acid bacteria pellets for feeding is prepared through the following steps;

[0040] (1) Preparation of lactic acid bacteria sludge: When Lactobacillus acidophilus is cultured in the MRS culture medium to the middle of the logarithmic growth phase, about 10 to 16 hours, a certain amount of sodium chloride is added to the respective culture medium to make the chlorine in the culture medium The final concentration of sodium chloride is controlled at 0.6Mmol / L; at this concentration, the lactic acid bacteria are continued to be cultivated until the early stage of the stable growth period, about 6-10 hours, centrifuged at a centrifugal acceleration of 4600g for 10 minutes, and the bacteria sludge is washed with 0.9% normal saline Twice, collect the sludge, then resuspend the sludge in fresh MRS medium, slowly raise the temperature to 50°C, and keep it at this temperature for 30 minutes, then quickly cool to 0-4°C, and set aside;

[0041] (2) We...

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Abstract

The invention provides a preparation method of feeding lactic acid bacteria micro pills. The preparation method comprises the following steps: culturing lactic acid bacteria until the metaphase of a logarithmic growth phase appears; adding sodium chloride into a culture solution to perform continuous culture until the prophase of a stable growth phase appears; centrifuging; cleaning bacterial sludge by using normal saline, and collecting the bacterial sludge; re-suspending the bacterial sludge in a fresh MRS culture medium, slowly heating to 45-55 DEG C, keeping the temperature for 30 minutes, then quickly cooling to 0-4 DEG C, taking out the bacterial sludge, and uniformly mixing the bacterial sludge with an appropriate amount of a filling agent, sodium alginate, lactalbumin, xylooligosaccharide, fructo-oligosaccharide, resistant starch and an antioxidant to obtain a mixture; and then putting the mixture into an extrusion pill-rolling machine for pelletizing, and drying after pelletizing to obtain the feeding lactic acid bacteria micro pills. The preparation method can be used for effectively improving the stability and tolerance of the lactic acid bacteria in the micro pills, so that the survival rate of the lactic acid bacteria during storage, transportation and processing of the lactic acid bacteria micro pills is increased and the living bacterium rate of a feed is increased; and meanwhile, the preparation method is simple and easy to control, and is suitable for industrial large-scale production.

Description

technical field [0001] The present invention relates to the technical field of lactic acid bacteria preparations, in particular to a preparation method of lactic acid bacteria pellets for feeding. Background technique [0002] Lactic acid bacteria are normal flora in the intestinal tract of animals. Facultative anaerobic lactic acid bacteria can produce lactic acid, diacetyl, hydrogen peroxide, bacteriocin and other substances, which can inhibit pathogenic bacteria and spoilage bacteria, maintain intestinal micro-ecological balance, prevent diarrhea, improve Immunity, improving the quality and physiological effects of animal products. Therefore, it has become an urgent need for the feed industry. Most lactic acid bacteria have good storage stability under low temperature conditions, but most feed companies do not have cold chain conditions, so lactic acid bacteria usually die in large numbers during storage and transportation; on the other hand, feed companies , are accomp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16
Inventor 邹强谢建将段振楠董世容
Owner SICHUAN GAOFUJI BIOLOGICAL TECH
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