Method for building full-thickness skin models

Abstract

The invention relates to a method for building full-thickness skin models. The full-thickness skin models comprise corium layers and epidermal layers. The corium layers comprise fibroblasts, the epidermal layers comprise epidermal cells, and substances such as cosmetics can be detected on the full-thickness skin models instead of the skin tissues of animals. The method has the advantages that cells of the corium layers are inoculated in gradient time, and can secrete a large quantity of collagen and form mature extracellular matrixes under the joint effects of V<c> (vitamin C), natural three-dimensional supports and growth spaces can be provided for the cells by the extracellular matrixes in cell re-layering procedures, and the biological activity and the biocompatibility of the cells can be improved; repeated inoculation and cell proliferation re-layering procedures are combined with one another, so that the thicknesses of the corium layers can be increased, and the mechanical strength and the stability of the corium layers can be improved; requirements on displaying the activity and biochemical functions of the cells of the corium layers and the epidermal layers can be met by nutrient solution after the corium layers and the epidermal layers are combined with one another.

Description

technical field [0001] The invention belongs to the technical field of tissue engineering medical biomaterials, and specifically relates to a safety and efficacy test for cosmetics, small molecular compounds, active proteins, medical devices, chemicals, disposable hygiene products, etc. A method for constructing a full-thickness skin model for safety and efficacy evaluation of skin-contact materials. Background technique [0002] Skin model refers to the skin substitute artificially prepared in vitro by using the principles and methods of engineering and cell biology. The full-thickness skin model is highly similar to the normal skin structure, with a complete epidermis and dermis structure. In addition to being used to repair and replace defective skin tissue, its greatest use is to replace human skin tissue for cosmetics, small molecular compounds, and active ingredients. Safety and efficacy evaluation of proteins, medical devices, chemicals, disposable hygiene products, ...

AI Technical Summary

Problems solved by technology

However, the artificial skin obtained by this method cannot completely replace skin tissue to meet the test and evaluation requirements for cosmetics, small molecular compounds, active proteins, medical devices, chemicals, and skin-contact materials.

The skin model constructed with scaffold materials and living cells is accompanied by the secretion of collagen of the living cells and the degradation of the scaffold materials during the culture process, resulting in shrinkage of the artificial skin tissue, which seriously affects the mass transfer efficiency in the in vitro construction of the full-thickness skin model, resulting in Shrinkage of the edge and separation of the culture vessel wall, and the inability to form a basement membrane structure consistent with natural skin between the eu-epidermal layers, both have an impact on the in vitro test results

[0004] At present, there is no scaffold material, only rely on the extracellular matrix secreted by the cells to form the dermis structure and the full-thickness skin structure formed by the combination of the epidermal structure that supports the growth of the dermis

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