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Plant immune induced resistance protein composition as well as preparation method and application thereof

A technology of immune-inducing protein and composition, which is applied in the field of plant immune-inducing protein composition, which can solve the problems of not being able to improve the resistance of tobacco mosaic virus disease, and achieve the effects of low cost, increased crop yield, and prolonged fresh-keeping period

Active Publication Date: 2015-07-01
北京绿色农华作物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these two proteins cannot cause allergic reactions in tobacco, and cannot improve the resistance of tobacco to tobacco mosaic virus disease; Drought resistance of wheat, induction of systemic resistance of tomato to Botrytis cinerea, can also induce allergic reactions of tobacco, significantly improving the ability of tobacco to resist mosaic virus disease

Method used

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  • Plant immune induced resistance protein composition as well as preparation method and application thereof
  • Plant immune induced resistance protein composition as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] Example 1 Comparison of Botrytis cinerea immune-inducing protein and several protein compositions against Botrytis cinerea

[0032] For each treatment, 20 tomato seeds were soaked in the above solution for 24 hours, and each treatment was repeated 3 times. 20 mM HEPES buffer was used as a control. After the seeds germinate, the seeds are sown in the nutrient bowl and placed in an incubator with suitable conditions. When the tomato grows to the 4-5 leaf stage, the Botrytis cinerea spore suspension (10 5 per mL), moisturized for 48 hours, and carried out disease-level investigation on the 14th day to calculate the induction effect.

[0033]

[0034] Induction effect (%) = (control condition index - treatment condition index) / control condition index × 100

[0035] Table 1: Botrytis cinerea immune-inducing protein and the effects of three protein treatments on tomato resistance to Botrytis cinerea

[0036] deal with

Disease index

Induction effect (...

example 2

[0039] Example 2 Botrytis cinerea plant immune-inducing protein induces pepper resistance to blight

[0040] For each treatment, 20 plants of peppers at the 5-6 leaf stage were sprayed with the above solution, and 20 mM HEPES buffer was used as a control, and each treatment was repeated 3 times. After 4 days of induction, inoculate the spore suspension of Phytophthora capsici (10 6 cells / mL), and cultured at 28°C for 48 hours. Three days later, the disease level investigation was carried out, and the induction effect was calculated (the method was the same as above).

[0041] Table 2: Effects of Botrytis cinerea plant immune-inducing protein on pepper resistance to blight

[0042] deal with

[0043] Botrytis cinerea plant immune-inducing protein and three protein combinations can induce the resistance of pepper to blight. Among them, the protein PebC1, PebC1+PebC2, PebC1+BcGS1, PebC1+PebC2+BcGS1 and Botrytis cinerea plant immune inducer protein diluted 100 times ...

example 3

[0044] Example 3 Botrytis cinerea plant immune-inducing protein induces tobacco resistance to mosaic virus

[0045] Each protein and its composition, Botrytis cinerea plant immune inducer protein were diluted 10 times, 100 times, 1000 times respectively and sprayed to treat the tobacco leaves at the 4-5 leaf stage, and inoculated with TMV after 3 days of induction. The inoculation method of TMV is as follows: take fresh TMV diseased leaves and add 0.01mol / L HEPES buffer (pH=7.4) to grind into a homogenate, and the concentration of the diseased juice is 15mL of HEPES buffer per gram of diseased leaves. The upper 3 leaves of each tobacco plant were rubbed and inoculated with TMV. With 20mM HEPES buffer as the control, each treatment of 10 strains was repeated 3 times. Three days after inoculation, the number of rotten spots was investigated, and the rotten spot inhibition rate was calculated.

[0046] Inhibition rate (%)=[(number of necrotic plaques / diameter in control group-n...

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Abstract

The invention relates to a plant immune induced resistance protein composition as well as a preparation method and application thereof and particularly relates to the plant immune induced resistance protein composition with a fungus source, application of the plant immune induced resistance protein composition in promoting growth of plants and inducing plants to produce immunity and a preparation method of the composition. According to the plant immune induced resistance protein composition, a protein product is separated and extracted from botrytis cinerea and comprises a mixture of at least two active proteins in three active protections with the molecular weights of 14kDa, 36KDa and 80kDa. A large number of tests show that the composition can promote the growth of various crops and the yield can be increased by more than 20%; the plants are induced to generate the immunity and the broad spectrum resistance to a plurality of pathogenic bacteria by the plants is enhanced; and the prevention and treatment effect is 40-82%. According to the plant immune induced resistance protein composition, the environment pollution and pesticide residue problems caused by chemical pesticides and fertilizers are solved, and the composition has very important meanings on improvement of the yield of crops and guarantees of national grain safety. The composition has the advantages of small use amount, convenience for utilization and long lasting period.

Description

technical field [0001] The invention relates to a plant immune-inducing protein composition, in particular to a protein composition derived from Botrytis cinerea that can promote plant growth and induce plant immunity, its preparation method and application, and belongs to the field of biotechnology . Background technique [0002] Improving crop yields and ensuring national food security are the foundation and primary task of my country's social stability and economic development. For a long time, plant diseases caused by pathogenic microorganisms have been one of the important factors threatening agricultural production in our country. Chemical control has greatly promoted the development of agricultural production, but it also seriously pollutes the environment, destroys biodiversity, and enhances the drug resistance of pathogenic bacteria. my country has become the world's largest producer and user of pesticides, and the average amount of chemical pesticides used per un...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01P21/00A01P3/00A01P1/00A01P17/00A01P13/00A23L3/3526C12P21/00C07K14/37C07K1/18C12R1/645
CPCY02A40/10
Inventor 张云华徐万涛张雄鹏李鹏邱德文杨秀芬
Owner 北京绿色农华作物科技有限公司
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