Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof

The technology of Bacillus cereus and Bacillus cereus is applied in the field of blueberry rhizosphere growth-promoting Bacillus cereus and its compound inoculants, and can solve the problems of few blueberry PGPR strains, blueberry rhizosphere growth-promoting bacterial strain resources and the like , to achieve the effect of promoting blueberry growth, strong pertinence and effectiveness

Active Publication Date: 2015-07-01
SHANDONG FOREST SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are very few studies on the screening and application of blueberry PGPR strains, and the resources of blueberry rhizosphere growth-promoting bacterial strains are very scarce.

Method used

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  • Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof
  • Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof
  • Blueberry rhizosphere growth-promoting bacillus cereus as well as composite microbial agent and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Screening of blueberry rhizosphere growth-promoting Bacillus cereus

[0038] (1) Screening process:

[0039] By gradient dilution method, 252 isolates were isolated from the blueberry rhizosphere soil samples of the blueberry production base of Joyvio Group Co., Ltd., Huangdao District, Shandong Province. Purify the isolated bacteria by the three-section line method, and judge whether the strain is purified by microscopic examination, number the purified bacteria, pick a single colony, and transfer it to an LB slant for future use. By measuring the ability of each bacterial isolate to produce auxin (IAA), decompose inorganic phosphorus, and produce protease, 4 strains with better effects were screened out, combined with the blueberry nutrient pot seedling test, and finally 1 strain with good growth-promoting effect was screened out. bacteria.

[0040] (2) Colony characteristics and cell morphology

[0041] Bacillus cereus L5 forms glossy white colonies afte...

Embodiment 2

[0053] Embodiment 2 has growth-promoting effect on blueberry growth

[0054] After the blueberry rhizosphere growth-promoting Bacillus cereus L5 and Bacillus fusiformis L13 were activated 2 to 3 times, a small amount of bacteria was picked with an inoculation loop and inoculated into 50 mL of medium (0.3% beef extract, 1% peptone, chlorine NaCl 0.5%, the balance is distilled water, pH 7.0-7.2, the above are mass percentages) in a 100mL Erlenmeyer flask, shake culture at 37°C and 180rpm for 2 days. Fermentation liquid was centrifuged at 6000rpm for 5min, after the bacterium was rinsed with sterile normal saline for 3 times, the bacterial suspension was adjusted with sterile normal saline (2.0×10 8 cfu / m) to make an inoculant. After planting the blueberry container seedlings, take 20ml and dilute them 10 times with sterile water and water them evenly around the blueberry roots. Three months later, measure the blueberry root system, seedling height and ground diameter, and the r...

Embodiment 3

[0056] The preparation of embodiment 3 microbial composite bacterial agents

[0057] Specific steps are as follows:

[0058] (1) the cultivation method of described blueberry rhizosphere growth-promoting bacillus cereus L5, bacillus fusiformis L13 (the following are percentages by weight):

[0059] ① Seed culture in test tube

[0060] The formula of the seed culture medium is: 0.3% of beef extract, 1% of peptone, 0.5% of sodium chloride, the balance is water, and the pH is 7.0-7.2.

[0061] The blueberry rhizosphere growth-promoting Bacillus cereus L5 and Bacillus fusiformis L13 were respectively inoculated on the culture medium, and cultured at a constant temperature of 30-35°C for 36 hours to obtain test-tube species.

[0062] ② Liquid fermentation culture

[0063] The formula of the liquid fermentation medium is: lactose 2%, peptone 2%, K 2 HPO 4 0.15%, MgSO 4 ·7H 2 O 0.02%, CaCl 2 0.01%, pH6.2-6.8, the balance is water.

[0064] Inoculate the test tube seeds of bl...

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Abstract

The invention discloses blueberry rhizosphere growth-promoting bacillus cereus as well as a composite microbial agent and an application thereof. The strain is classified and named as bacillus cereus (Bacillus cereus) L5, is preserved in China General Microbiological Culture Collection Center on 31, May in 2013 and has the preservation number of CGMCC No.7667. The invention also provides an application of the bacillus cereus, a microorganism composite microbial agent comprising the bacillus cereus, and a preparation method of the composite microbial agent. The blueberry rhizosphere growth-promoting bacillus cereus L5 disclosed by the invention can be successfully colonized in blueberry rhizosphere soil, and growth of blueberries can be promoted. According to the microorganism composite microbial agent disclosed by the invention, the optimized combination of the blueberry rhizosphere growth-promoting bacillus cereus L5, bacillus fusiformis L13, lactobacillus casei and other strains is realized, so that the blueberry composite microbial agent has high pertinence and effectiveness.

Description

technical field [0001] The invention relates to a blueberry rhizosphere growth-promoting bacillus cereus and its compound bacterial agent and application. Background technique [0002] Blueberry belongs to Rhododendron (Ericaceae) Vaccinium spp. plant, is a small perennial shrub species. The fruit has high nutritional and medical value, good fresh food and processing quality, and has high development and utilization value. [0003] Soil microorganisms are an important part of the soil ecosystem and play a key role in the decomposition of organic matter, nutrient cycling and plant nutrient utilization. Inoculation of soil microorganisms is generally recognized as an environmentally friendly and cost-effective method to improve crop yield and quality. [0004] The growth of blueberries requires strong acidic (pH 4.0-4.8) soil conditions. Under artificial cultivation conditions, the method of applying sulfur powder to the soil is generally used to adjust and maintain it. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P21/00C12R1/085C12R1/07C12R1/245
CPCA01N63/00C12N1/20C12N1/205C12R2001/085
Inventor 刘方春孙蕾马海林马丙尧杜振宇
Owner SHANDONG FOREST SCI RES INST
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