Application of rice genes OsVHA1 in delaying senility of plant leaves and improving plant salt tolerance

Abstract

The invention discloses application of rice genes OsVHA1 in delaying senility of plant leaves and improving plant salt tolerance. A nucleotide sequence of the rice genes OsVHA1 is shown in SEQ ID NO.1; the application comprises the processes that the rice genes OsVHA1 are joined with an expression carrier to construct and obtain a recombinant expression carrier; the recombinant expression carrier is converted into a receiver plant. Base on a tula cloning technology, the cloning from a rice premature senescence mutant osvha1 is carried out to obtain genes OsVHA1; a functional complementary experiment proves that the genes OsVHA1 can delay the senility of plant leaves while a salt processing experiment proves that the genes OsVHA1 can improve the salt tolerance capability of the plant; the genes OsVHA1 can be applied to the field of plant breeding and are significant in screening and selective breeding of plant varieties with senescence resistance and salt tolerance.

Description

technical field [0001] The invention relates to the technical field of plant genetic engineering and rice molecular breeding, in particular to the application of a rice gene OsVHA1 in delaying plant leaf senescence and improving plant salt tolerance. Background technique [0002] Rice is one of the most important food crops, and more than half of the world's population depends on rice as a staple food. In our country, with the continuous increase of population and the decrease of land area, the demand for increasing rice production is becoming more and more urgent, so the national food security strategy is particularly important. Therefore, cultivating high-yield and high-quality rice varieties and continuously increasing rice yields are the most important tasks to ensure my country's food security. Leaf is the most important photosynthetic organ of rice. According to theoretical calculations, 60% to 80% of the nutrients in rice grain filling come from the photosynthesis of...

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Problems solved by technology

However, existing studies have shown that it is only involved in the regulation of some traits. For example, the loss of function of subunit A in Arabidopsis can lead to complete male sterility and lethality of some female gametophytes (Dettmer J, Schubert D, Calvo-Weimar O, Stierhof YD, Schmidt R, Schumacher K. Essential role of the V-ATPase in male gametophyte development. Plant J. 2005, 41: 117-122), while antisense inhibition of carrot A subunit resulted in smaller cells and more leaf division Multiple (Gogarten J P, Fichmann J, Braun Y, et al. The use of antisense mRNA to inhibit the tonoplast H+-ATPase in carrot. The Plant Cell, 1992, 4: 851-864), antisense inhibits the A1 subunit of rice then only lead to increased leaf stomatal density and sensitivity to salt and drought (Zhang H, Niu X, Liu J, Xiao F, Cao S, Liu Y. RNAi-directed downregulation of vacuolar H(+)-ATPase subunit a results in enhanced stomatal aperture and density in rice.PLOS ONE.2013,8:e69046), and overexpression of apple A subunit in tobacco can improve the salt tolerance of transgenes (Dong QL, Wang CR, Liu DD, Hu DG, Fang MJ , You CX, Yao YX, Hao YJ. MdVHA-A encodes an apple subunit A of vacuolar H(+)-ATPase and enhances drought tolerance in transgenic tobacco seedlings. J Plant Physiol. 2013, 170: 601-609)

[0006] So far, although many existing studies have shown that the A subunit plays a very important role in plant growth and development, there has been no report on its regulation of plant leaf senescence

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