Preparation method of gardenia blue pigment

A technology of gardenia blue pigment and gardenia leaves, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of complex extraction process, high cost of raw materials, low yield and the like, and achieves simple and convenient process, The effect of low product cost and abundant sources

Active Publication Date: 2015-07-01
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent physical and chemical properties, and remarkable dyeing effect since its appearance. Increasingly, however, the traditional preparation method is to extract from gardenia fruit, and gardenia fruit contains a large amount of interfering substances, resulting in complex extraction process, excessive material consumption, low yield, and high raw material cost, the product Extremely expensive, which limits the large-scale application of such ingredients and the development of related industries in my country in recent years

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0062] 1. Preparation of gardenia leaf culture medium

[0063] Will 2 00 Kg Fresh Gardenia ( Gardenia jasminoides Ellis) leaves were washed, dried, crushed and passed through a 10-mesh sieve, sterilized at 100°C for 100 minutes, and cooled for later use to obtain a solid culture medium for gardenia leaves 2 00 Kg.

[0064] ． Aspergillus liquid seed preparation

[0065] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, kept at a constant temperature of 25°C, and cultured for 4 days to obtain the activated stock;

[0066] (2) Inoculate the activated stock into the liquid seed medium

[0067] Each liter of liquid seed medium consists of 5 g of peptone, 10 g of starch, 1 g of potassium dihydrogen phosphate, 1 g of dipotassium hydrogen phosphate, and 1 g of magnesium sulfate, which are dissolved in water to 1 L.

[0068] Fill the t...

Embodiment 2

[0078] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0079] 1. Preparation of gardenia leaf culture medium

[0080] 200 kg of dried water gardenia with a moisture content of 20% ( Gardenia jasminoides var. radicans Makino ) leaves, crushed through a 100-mesh sieve, mixed with water for 5 minutes according to the material-to-liquid ratio of 1Kg:0.2L, sterilized at 120°C for 20 minutes, and cooled to obtain 240 kg of gardenia leaf solid medium;

[0081] 2． Aspergillus liquid seed preparation

[0082] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, and cultured at 35°C for 7 days to obtain the activated stock;

[0083] (2) Inoculate the activated stock into the liquid seed medium

[0084] Each liter of liquid seed medium is prepared by adding 30 g of yeast extract, 50 g of sucrose, 3 g of potassium dihydrogen phosphate, 3 g of dipotassium hydrogen phosphate, and 3 g of magnesium sulf...

Embodiment 3

[0095] The specific operation steps of preparing gardenia blue pigment from gardenia leaves are as follows:

[0096] 1． Preparation of gardenia leaf culture medium

[0097] 200 kg of fresh gardenia ( Gardenia jasminoides Ellis) Gardenia leaves, crushed through a 50-mesh sieve, mixed with water for 30 minutes according to the material-to-liquid ratio of 1Kg:0.5L, sterilized at 110°C for 50 minutes, and cooled to obtain 300 kg of Gardenia leaf liquid culture medium;

[0098] 2． Aspergillus liquid seed preparation

[0099] (1) Aspergillus niger ( Aspergillus niger ) was inoculated on SDAY slant medium, kept at a constant temperature of 30°C, and cultured for 5 days to obtain the activated stock;

[0100] (2) Inoculate the activated stock into the liquid medium

[0101] Each liter of liquid seed culture medium is prepared by adding 15 g of yeast extract, 25 g of sucrose, 2 g of potassium dihydrogen phosphate, 2 g of dipotassium hydrogen phosphate, and 2 g of magnesium sulf...

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PUM

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Abstract

The invention discloses a preparation method of gardenia blue pigment. The preparation method comprises the following steps: (1) preparing a cape jasmine leaf culture medium, namely, preparing a solid culture medium and preparing a liquid culture medium; (2) preparing aspergillus liquid seeds, namely, preparing primary seeds, secondary seeds and tertiary seeds; (3) inoculating and fermenting: carrying out liquid-solid bi-directional fermentation and liquid fermentation; (4) carrying out enzymolysis on geniposide: carrying out enzymolysis for liquid-solid bi-directional fermented substance and a liquid fermented substance; (5) generating and purifying the gardenia blue pigment. The geniposide aglycone and ammonia acid are condensed to generate the gardenia blue pigment by adopting the heating of the ammonia acid, the pigment purification is carried out by utilizing a resin column, finally the black blue powder is obtained, i.e. the gardenia blue pigment with color value more than 190 (E1%1cm 590nm). The gardenia blue pigment is produced by adopting the cape jasmine leaves, so that the production cost is greatly reduced; the process is simple and stable, the investment on the production equipment is variable, the flexibility in production can be realized, and the preparation method is suitable for the enterprise investment of various scales.

Description

technical field [0001] The invention belongs to the technical field of extraction and preparation of biological products, and in particular relates to a preparation method of gardenia blue pigment. Background technique [0002] Gardenia blue pigment was initially produced by hydrolyzing geniposide in gardenia fruit and reacting with amino acids. Gardenia blue pigment is soluble in water and low-alcohol and other hydrophilic solvents. It has excellent physical and chemical properties such as light and heat resistance, acid and alkali resistance, and unique dyeing effect. Gardenia blue pigment can not only replace synthetic pigments by itself, but can also be used in harmony with red and yellow pigments of other strains, and can be blended into a series of blue-green hues such as green, purple, and brown. Gardenia blue pigment has been widely used in many fields, such as food and medicine, due to its natural source, safety, non-toxicity, good human compatibility, excellent ph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02C12R1/685
Inventor 胡丰林
Owner ANHUI AGRICULTURAL UNIVERSITY
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