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Glutathione yeast enzymolysis solution composition and preparation method thereof

A glutathione and yeast enzymatic hydrolysis technology, which is applied in food preparation, food science, application, etc., can solve the problems of toxic side effects, short product life cycle, and many chemical residues, so as to ensure reducibility, rich types and proportions And balance, the effect of protecting the liver

Inactive Publication Date: 2015-07-08
四川安益生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, the health care product market is mixed with good and evil people, most of the health care products exaggerate their effects, and the product life cycle is short
Moreover, the vast majority of health care products currently use various food-grade and pharmaceutical-grade biochemical raw materials as components, and are artificially blended, which has the risk of many chemical residues and is often accompanied by toxic and side effects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] (1) Fermentation and cultivation of Saccharomyces cerevisiae strains (5 liters of fermentation volume) in 10 liters of fermenters, the optimized medium consists of (g / L): glucose 80, sodium citrate 10, ammonium sulfate 7, potassium dihydrogen phosphate 3.5, Magnesium sulfate 0.5 (the same below). During the fermentation process (within 10 to 20 hours), 5g of glutamic acid (1g / L), 2.5g of glycine (0.5g / L), and 4g of cysteine ​​(0.8g / L) were added to ferment to the end (That is, the highest glutathione titer: up to 8mg GSH / gram of dry yeast) to obtain glutathione yeast; yeast wet weight 50g / L; fermentation time 32 hours.

[0042] (2) Glutathione yeast was centrifuged at 6000rpm for 5 minutes, washed 3 times, and resuspended in pure water to obtain 600mL of system A containing 80g / L of glutathione yeast dry matter, and adjusted its pH to 4.0, 70MPa with lactic acid High-pressure homogenization for 2 times, adding 0.14 g of yeast extract accounting for 0.3% by weight of th...

Embodiment 2

[0049] (1) Saccharomyces cerevisiae strains (12 liters of fermentation volume) were fermented and cultured in a 30-liter fermenter, and the optimized medium composition was the same as that in Example 1. During the fermentation process (within 10-20 hours), 15.6g of glutamic acid (1.3g / L), 7.2g of glycine (0.6g / L), and 7.2g of cysteine ​​(0.6g / L) were added, Ferment to the end (that is, the highest glutathione titer: up to 9mg GSH / g dry yeast) to obtain glutathione yeast; yeast wet weight 56g / L; culture time 42 hours.

[0050] (2) Glutathione yeast was centrifuged at 8000rpm for 3 minutes, washed twice, resuspended in pure water to obtain 2000mL of system A containing 70g / L of glutathione yeast dry matter, adjusted to pH 3.6, and 75MPa high pressure homogenization 2 times, adding 0.28 g of yeast extract 0.2% by weight of the solid content of system A and 0.28 g of 0.2% NaCl, at 59° C., incubated for 3 hours to obtain system B.

[0051] (3) Add oyster fine powder 90g (45g / L) (...

Embodiment 3

[0057] The specific implementation plan is:

[0058] (1) Saccharomyces cerevisiae strains (50 liters of fermentation volume) were fermented and cultured in a 100-liter fermenter, and the composition of the optimized medium was the same as in Example 1. During its fermentation (within 10-20 hours), 45g glutamic acid (0.9g / L), 27.5g glycine (0.55g / L), 25g cysteine ​​(0.5g / L) were added, and fermented to End point (glutathione titer is the highest: 7.8mg GSH / g dry yeast), obtain glutathione yeast, the wet weight of yeast is 60g / L; Culture time 45 hours.

[0059] (2) Glutathione yeast was centrifuged at 6000rpm for 5 minutes, washed 3 times, resuspended in pure water to obtain 7000mL of System A containing 85g / L of glutathione yeast dry matter, and succinic acid adjusted its pH to 3.0, 55MPa Homogenize under high pressure for 3 times, add 2.08 g of yeast extract with a weight of 0.35% of the solid content of system A and 0.9 g of 0.15% NaCl, heat at 60° C. for 4 hours, and obtain...

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Abstract

The invention provides a glutathione yeast enzymolysis composition and a preparation method thereof. The method is characterized by the following steps: (1) fermenting for culture saccharomyces cerevisiae to obtain glutathione yeast; (2) resuspending the glutathione yeast to obtain a system A, adjusting the pH value of the system A, and carrying out high pressure homogenization wall breaking and induced autolysis to obtain a system B; (3) adding an oyster fine powder and an acidic protease into the system B for conducting enzymatic hydrolysis on yeast and oyster protein; (4) centrifuging the system C and removing the yeast cell wall and impurities to obtain a system D; and (5) adding accessories into the system D, conducting vacuum concentration and vacuum drying to obtain the glutathione yeast enzymolysis composition. The composition can be prepared into any dosage form such as tablets or filled capsules, has the efficacies of protecting liver, detoxifying and resisting oxidation and fatigue, and is also an effective human nutrition enhancement supplement.

Description

technical field [0001] The invention belongs to the field of functional foods, and in particular relates to a glutathione yeast enzymolysis composition and a preparation method thereof. Background technique [0002] At present, the health care product market is mixed with good and evil people, most of the health care products exaggerate their effects, and the product life cycle is short. Moreover, the vast majority of health care products currently use various food-grade and pharmaceutical-grade biochemical raw materials as components, and are artificially combined, which has the risk of many chemical residues and is often accompanied by toxic and side effects. [0003] For thousands of years, yeast has been one of the natural health foods for human beings. Eating yeast itself can provide most of the nutrients needed by the human body, and is an ideal source of protein and vitamin supplements for humans. Glutathione is a natural product in yeast cells, which has various ph...

Claims

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Application Information

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IPC IPC(8): A23L1/305A23L1/302A23L1/304A23L33/15
Inventor 叶春江姜均
Owner 四川安益生物科技有限公司
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